Abstract: Provided herein are magnetofluidic cartridges of use in a wide variety of sample analysis applications, including nucleic acid amplification assays. The magnetofluidic cartridges include sample inlet wells and sample analysis wells for performing controlled serial elution techniques that enables execution of extraction/purification and splitting of analytes for multiplex detection via magnetic actuation only. Related magnetofluidic devices, kits, and methods are also provided.

Abstract: Systems, devices, and methods are described herein for improving inventory management. As used herein, “inventory” refers to digital space at an inventory providers webpage at which content can be delivered. The disclosed techniques utilize reinforced machine learning and an offline training process to train various models with which a content request corresponding to the inventory can be classified according to historical requests and a selection process identified for the request (e.g., a direct or an indirect selection process). If an indirect selection process is chosen, the content request may be optimized for that process utilizing additional machine learning models trained using reinforced machine learning and the offline training process. The disclosed techniques enable the inventory provider to optimize content selections according to a preferred objective. The training operations are performed offline, in a training system configured to simulate the run time environment.

Abstract: Provided herein are magnetofluidic cartridges of use in a wide variety of sample analysis applications, including nucleic acid amplification assays. The magnetofluidic cartridges include sample inlet wells and sample analysis wells. Temperature sensitive materials are used to separate the sample inlet wells and sample analysis wells from one another prior to performing a given sample analysis application. Related magnetofluidic devices, kits, and methods are also provided.

Abstract: A tubing-free, sample-to-droplet microfluidic system includes a tubing-free, sample-to-droplet microfluidic chip; a valve control system connected to the tubing-free, sample-to-droplet microfluidic chip; a vacuum system fluidly connected to the tubing-free, sample-to-droplet microfluidic chip; and a droplet formation pressure system fluidly connected to the tubing-free, sample-to-droplet microfluidic chip. A microfluidic chip for a tubing-free, sample-to-droplet microfluidic system includes a tubing-free, sample-to-droplet interface section; a droplet mixing section in fluid connection with the tubing-free, sample-to-droplet interface section to received droplets therefrom; an incubation section in fluid connection with the droplet mixing section to receive droplets therefrom; and a detection section in fluid connection with the incubation section to receive droplets therefrom.

Abstract: A system and method for enabling users to operate multiple blockchain (or distributed ledger) nodes efficiently, at a much lower cost, etc. For example, when one blockchain starts to take up resources, the system can automatically adjust by looking at the application container layer to see if it is possible to shift resources. It can also shift resources at the server layer to adjust for computation and memory resources to, for example, increase efficiency. It may also share storage for nodes that share the same blockchain to, for example, increase efficiency. The system can also identify tokens that are more efficient to stake, and to shift tokens from one blockchain to another for staking reward efficiencies, etc. It may also deploy one node to another geographic region to increase decentralization, as well as adjust rewards and commissions to provide incentives to boost decentralization as well.

Abstract: Provided herein are microfluidic devices for analyzing samples. In one aspect, the microfluidic device includes a body structure having a droplet compression chamber, a sieve structure in fluid communication with the droplet compression chamber, which sieve structure comprises an array of protrusions that extend from at least one surface of the body structure and define at least a portion of one or more fluidic circuits, and a port at least partially disposed in the body structure. Other aspects include kits, methods, systems, computer readable media, and related aspects for analyzing samples.

Abstract: Presented herein are systems, methods, and datasets for automatically and precisely generating highlight or summary videos of content. In one or more embodiments, the inputs comprise a text (e.g., an article) of the key event(s) (e.g., a goal, a player action, etc.) in an activity (e.g., a game, a concert, etc.) and a video or videos of the activity. In one or more embodiments, the output is a short video of an event or events in the text, in which the video may include commentary about the highlighted events and/or other audio (e.g., music), which may also be automatically synthesized.

Abstract: A back plate configured to support a circuit board includes a body. The body defines a central recess formed into a top surface thereof, and a raised main support column extending from the central recess and adapted to support the circuit board.

Abstract: Methods for multiplexed detection of a nucleic acid sequence in a sample including the use of a plurality of oligonucleotide target-specific probes (TSPs) configured to bind to a distinct target nucleic acid sequence, where each of the TSPs includes one or more copies of a first fluorescent probe (FP) binding region and one or more copies of a second FP binding region, and where a predetermined ratio of the one or more copies of the first FP binding region to the one or more copies of the second FP binding region is indicative of the distinct target nucleic acid sequence the TSP is configured to bind to.

Abstract: Provided herein are methods of processing grain samples to detect the fractional abundance of transgenic traits. Related systems and computer program products are also provided.

Abstract: Provided herein are methods of performing multiplexed real-time quantitative ratiometric regression PCR (qRR-PCR) to determine the fractional abundance of target variants in a population. Related systems and computer program products are also provided.

Abstract: A system and method for enabling users to operate multiple blockchain (or distributed ledger) nodes efficiently, at a much lower cost, etc. For example, when one blockchain starts to take up resources, the system can automatically adjust by looking at the application container layer to see if it is possible to shift resources. It can also shift resources at the server layer to adjust for computation and memory resources to, for example, increase efficiency. It may also share storage for nodes that share the same blockchain to, for example, increase efficiency. The system can also identify tokens that are more efficient to stake, and to shift tokens from one blockchain to another for staking reward efficiencies, etc. It may also deploy one node to another geographic region to increase decentralization, as well as adjust rewards and commissions to provide incentives to boost decentralization as well.

Abstract: The invention provides compositions and methods for detecting a neoplasia (e.g., pancreatic cancer, lung cancer, colon cancer) in a subject sample (e.g., serum, blood, plasma, tissue). In particular embodiments, the invention provides methods for detecting BNC1 and ADAMTS1 promoter methylation in circulating DNA in serum.

Abstract: A method and apparatus for a data analysis system for analyzing data object collections that include geo-temporal data is provided. One or more temporal granularities are specified for the purpose of generating a geo-temporal data index. The time granularities correspond to temporal ranges expected to correspond to temporal ranges specified in user queries against the data. One or more temporal index bucket groups are generated based on to the specified time granularities. Geo-temporal input data is indexed based on the generated temporal index bucket groups. The system allows a data analyst to specify geo-temporal queries that include both geospatial component and a temporal component. The system transforms geo-temporal queries into one or more second queries that retrieve data items based on the temporal index bucket groups.

Abstract: A number of variations may include a method that may include laser shock peening a friction work surface of a working part and applying a ferritic nitrocarburizing process to the friction work surface such that diffusion of carbon and nitrogen atoms into the friction work surface is accelerated.

Abstract: Described are methods for identifying antibiotic resistant bacteria, quantifying bacteria growth, and applying an antibiotic susceptibility test (AST) in one or more biological samples containing a bacteria and chips used in these methods.

Abstract: The present invention relates to the use of nucleic acid methylation and methylation profiles to detect risk of developing neoplasia and in particular, lung cancer. The invention relates to methods for identifying a methylation profile of the CDO1, SOOX17, HOXA7, HOXA9, TAC1, and ZFP42 genes from plasma and sputum samples.

Abstract: The disclosure provides a cushion including an outer cover defining an interior cavity, and inner filler material disposed within the interior cavity. The inner filler material may comprise at least one molded block of polyurethane foam. The outer cover and/or the inner filler material may be porous to at least water. The cushion further includes a waterproof coating to the outer surface the inner filler material and forming waterproof seal extending entirely thereabout after curing. The waterproof coating comprises butyl acrylate within the range of 17-32 wt %, styrene within the range of 13-26 wt %, and deionized water within the range of 42-70 wt % prior to curing. The waterproof coating may further include auxiliary components within the range of 0.5-8 wt % prior to curing. The auxiliary components may include acrylic acid, sodium lauryl sulfate, an emulsifier component, ammonium peroxydisulfate, sodium bicarbonate, sodium phosphate, tertiary and tributyl phosphate.

Abstract: The invention provides compositions and methods for detecting a neoplasia (e.g., pancreatic cancer, lung cancer, colon cancer) in a subject sample (e.g., serum, blood, plasma, tissue). In particular embodiments, the invention provides methods for detecting BNC1 and ADAMTS1 promoter methylation in circulating DNA in serum.

Abstract: A number of variations may include a method that may include laser shock peening a friction work surface of a working part and applying a ferritic nitrocarburizing process to the friction work surface such that diffusion of carbon and nitrogen atoms into the friction work surface is accelerated.