Abstract: Disclosed herein are compositions, devices, methods, processes, and systems for culturing of large quantities of mammalian cells in suspension culture. Also disclosed are unique and surprising cell populations derived from the disclosed methods, processes, and systems. In many embodiments, the cells are cultured in suspension in liquid culture media that is agitated to maintain the cells in suspension, and agitation increases to maintain cells in suspension while growing and dividing to create cell spheres. The disclosed devices, methods, processes, and systems are useful in tailoring characteristics of the resulting cells based on characteristics of donor subject/initial cells. The disclosed cell populations are useful in treating subjects with cell based therapies in need thereof for various diseases and conditions.

Abstract: A motorized vehicle is provided for transporting material and selectively dumping the material from the vehicle. In one example, the motorized vehicle includes a frame, first and second forward wheel assemblies coupled to the frame, and a rearward wheel assembly coupled to the frame. An engine is supported by the frame and configured to rotate the first and second forward wheel assemblies to move the motorized vehicle. The motorized vehicle also includes a bucket supported by the frame adjacent the first and second forward wheel assemblies. The bucket is configured to rotate about a vertical axis between a first radial positon in which a longitudinal axis of the bucket is generally parallel with the longitudinal axis of the frame and a second radial positon in which the longitude axis of the bucket is oriented generally perpendicular to the longitudinal axis of the frame.