Abstract: A multi-channel microfluidic blood coagulation detection chip includes a chip body. The chip body includes a lower-layer chip, a middle-layer chip, and an upper-layer chip in sequence from bottom to top. The lower-layer chip, the middle-layer chip, and the upper-layer chip cooperate with each other to define a closed microfluidic channel and a plurality of mutually-independent detection chambers. The upper-layer chip is provided with a sample loading hole, and the sample loading hole communicates with the detection chambers through the microfluidic channel. The chip body further includes electrodes. The electrodes include upper-layer electrodes and lower-layer electrodes, the upper-layer electrodes are disposed on a back surface of the upper-layer chip, the lower-layer electrodes are disposed on a front surface of the lower-layer chip, and a gap is provided between the upper-layer electrodes and the lower-layer electrodes.

Abstract: Disclosed are a method for whole blood filtration and a filter membrane structure for whole blood filtration, specifically including the following steps: (1) a filter membrane structure made up of at least two filtration membranes sequentially stacked from top to bottom is selected, and subjected to hemagglutinin treatment for later use; (2) a whole blood sample is added to the filter membrane structure for filtration; and (3) the filtered serum or plasma is collected. The filter membrane structure is composed of at least two filtration membranes stacked from top to bottom, and the pore sizes of the filtration membranes stacked gradually decrease from top to bottom, and the areas of the same gradually increase or are equal to each other from top to bottom.

Abstract: The disclosure relates to an electrochemical detection method based on screen-printed electrodes, which specifically includes the following steps: Preparation of S1 protein-binding group: The working electrode on the screen-printed electrode substrate is processed, and the protein-binding group is adhered; S2 basic protein reaction: Add basic protein, and the active group on the basic protein reacts with the described The protein-binding group in step S1 reacts; S3 blocking the basic protein: uses a blocking solution to block the basic protein after the reaction in step S2 to obtain a screen-printed electrode to be detected; S4 electrochemical detection: add a detection reagent containing the target protein, the basic protein reacts specifically with the target protein, and the data collected by the working electrode after the reaction are electrochemically analyzed to obtain the detection result.

Abstract: Provided is a preparation method for an immunoelectrode. The immunoelectrode comprises a substrate, a gold layer, a conductive polymer layer and an antibody layer. The substrate, the gold layer, the conductive polymer layer and the antibody layer are sequentially attached from bottom to top. The preparation method for the immunoelectrode specifically comprises the following steps: (1) preparing the conductive polymer layer: preparing a polypyrrole layer on a gold-plated substrate to obtain a polypyrrole/gold-plated substrate; (2) preparing the immunoelectrode: preparing the antibody layer on the polypyrrole layer to obtain an antibody/polypyrrole/gold-plated substrate; and (3) forming an immunoelectrode system: fixing a bare gold-plated substrate to the outer side of the antibody/polypyrrole/gold-plated substrate to obtain the immunoelectrode system. A polypyrrole material is used for fixing an antibody of a biological recognition element and immobilizing the antibody on the immunoelectrode.

Abstract: A multi-channel microfluidic blood coagulation detection chip having a five-layer structure includes a chip body. The chip body includes, in sequence from top to bottom, a first-layer chip, a second-layer chip, a third-layer chip, a fourth-layer chip, and a fifth-layer chip. The first-layer chip (1), the second-layer chip, the third-layer chip, the fourth-layer chip, and the fifth-layer chip cooperate with each other to define a closed microfluidic channel and a plurality of mutually-independent detection chambers. The first-layer chip is provided with a sample loading hole, and the sample loading hole communicates with the detection chambers through the microfluidic channel. The chip body further includes electrodes, and the electrodes are disposed within the detection chambers in one-to-one correspondence.

Abstract: The invention discloses a microfluidic blood type detection chip.

Abstract: A detection system for multi-index coagulation items, including a housing, a detection and heating module, a battery module, a touch display screen and a mainboard. The housing includes an upper housing and a lower housing, and is an enclosed space formed by the upper housing abutting the lower housing; the mainboard is disposed between the upper and the lower housing, and is arranged in the enclosed space; the touch display screen is disposed at the upper surface of upper housing, and is connected with the mainboard; the detection and heating module is disposed in the enclosed space, and is arranged at the front end of lower housing for connecting with the mainboard; the battery module is arranged at the bottom of terminal of lower housing, and is connected with the mainboard; and a detection inlet for inserting and placing a detection card is disposed at the front end of housing.

Abstract: An electrochemical test base and apparatus for activated clotting time (ACT) is provided. The base includes a chip inlet. A magnetic field change structure is disposed above or below a position of the chip inlet. An ACT test chip is inserted from the chip inlet. The magnetic field change structure uniformly mixes a blood sample and a reactive reagent in the ACT test chip. The ACT test chip added with the blood sample is inserted from the chip inlet of the base. The magnetic field change structure is disposed above or below the position of the chip inlet, and applies a magnetic field to the chip. The magnetic field change structure can generate a changing magnetic field. An inertial magnetic rotating rod in a chip test cavity uniformly mixes the blood sample and the reactive reagent in the chip for thorough reaction, to ensure accurate test results, thereby resolving problems of the hysteresis and poor stability of test results.

Abstract: A biochemical analyzer is disclosed. The biochemical analyzer includes a detection disc sample feeding device, a detection disc device, an optical path detection device, and a temperature control device. The detection disc device includes a rotatable detection disc. The detection disc has a plurality of partitions, and each partition is correspondingly equipped with one chip to be detected. The detection disc is further provided with an optical limiting slot, and the optical limiting slot cooperates with a first limiting optocoupler located on a side face of the detection disc to monitor the number of rotations of the detection disc. Each partition of the detection disc may be equipped with one chip to be detected, and multiple chips can be detected in one operation by using the rotatable detection disc.

Abstract: The invention discloses a multifunctional microfluidic detection chip.

Abstract: A method for assigning an antibody standard and determining a minimum detection limit of an antibody detection reagent specifically includes the following steps: S1, determination of a neutralized antigen equivalent of an antibody standard or a sample: an antigen with a known purity and concentration is subjected to a gradient dilution using a matrix, an equal amount of an antibody standard or antibody-containing sample is added to each of the gradient diluents with different concentrations respectively, and after a reaction, each mixture is subjected to an antibody detection using a first antibody detection reagent, to determine the neutralized antigen equivalent of the antibody standard or sample; S2, determination of an antibody titer of the sample; and S3, acquisition of the minimum detection limit of an antibody detection reagent.

Abstract: Disclosed is a fully-automatic chemiluminescence immunoassay analyzer, which comprises a base, a reaction cup conveyance module, a robot-arm sample injection module, an incubation module, a manipulator and transit module, a magnetic-separation cleaning module, and a detection module are mutually independently integrated on the base. The reaction cup conveyance module is used for conveying a reaction cup to a pre-determined position; the manipulator and transit module carries the reaction cup into the incubation module, to incubate the reaction cup for a constant-temperature reaction; the reaction cup in the incubation module is carried to the magnetic-separation cleaning module for magnetic-adsorption cleaning; and the reaction cup after cleaning is carried by the manipulator and transit module to the detection module for detection. A plurality of manipulators and a plurality of transit devices may be disposed as required, to link the mutually independent functional parts of the modules.

Abstract: Provided is a preparation method for an immunoelectrode. The immunoelectrode comprises a substrate, a gold layer, a conductive polymer layer and an antibody layer. The substrate, the gold layer, the conductive polymer layer and the antibody layer are sequentially attached from bottom to top. The preparation method for the immunoelectrode specifically comprises the following steps: (1) preparing the conductive polymer layer: preparing a polypyrrole layer on a gold-plated substrate to obtain a polypyrrole/gold-plated substrate; (2) preparing the immunoelectrode: preparing the antibody layer on the polypyrrole layer to obtain an antibody/polypyrrole/gold-plated substrate; and (3) forming an immunoelectrode system: fixing a bare gold-plated substrate to the outer side of the antibody/polypyrrole/gold-plated substrate to obtain the immunoelectrode system. A polypyrrole material is used for fixing an antibody of a biological recognition element and immobilizing the antibody on the immunoelectrode.

Abstract: A method for assigning an antibody standard and determining a minimum detection limit of an antibody detection reagent specifically includes the following steps: S1, determination of a neutralized antigen equivalent of an antibody standard or a sample: an antigen with a known purity and concentration is subjected to a gradient dilution using a matrix, an equal amount of an antibody standard or antibody-containing sample is added to each of the gradient diluents with different concentrations respectively, and after a reaction, each mixture is subjected to an antibody detection using a first antibody detection reagent, to determine the neutralized antigen equivalent of the antibody standard or sample; S2, determination of an antibody titer of the sample; and S3, acquisition of the minimum detection limit of an antibody detection reagent.

Abstract: A microfluidic detection chip for multi-channel rapid detection, including a chip body. A chip sampling port, a plurality of independent detection chambers, and a microfluidic channel are disposed on the chip body, and the chip sampling port is connected to the detection chambers by means of the microfluidic channel. The chip body further includes an electrode. The detection chambers are connected to the electrode. The microfluidic channel includes a main flow channel and a plurality of branching microfluidic channels. A tail end of the main flow channel is divided into the plurality of branching microfluidic channels, and the plurality of branching microfluidic channels are connected to the plurality of independent detection chambers in a one-to-one corresponding manner. And, the other end of the main flow channel is connected to the chip sampling port.

Abstract: A multi-channel microfluidic blood coagulation detection chip includes a chip body. The chip body includes a lower-layer chip, a middle-layer chip, and an upper-layer chip in sequence from bottom to top. The lower-layer chip, the middle-layer chip, and the upper-layer chip cooperate with each other to define a closed microfluidic channel and a plurality of mutually-independent detection chambers. The upper-layer chip is provided with a sample loading hole, and the sample loading hole communicates with the detection chambers through the microfluidic channel. The chip body further includes electrodes. The electrodes include upper-layer electrodes and lower-layer electrodes, the upper-layer electrodes are disposed on a back surface of the upper-layer chip, the lower-layer electrodes are disposed on a front surface of the lower-layer chip, and a gap is provided between the upper-layer electrodes and the lower-layer electrodes.

Abstract: A multi-channel microfluidic blood coagulation detection chip having a five-layer structure includes a chip body. The chip body includes, in sequence from top to bottom, a first-layer chip, a second-layer chip, a third-layer chip, a fourth-layer chip, and a fifth-layer chip. The first-layer chip (1), the second-layer chip, the third-layer chip, the fourth-layer chip, and the fifth-layer chip cooperate with each other to define a closed microfluidic channel and a plurality of mutually-independent detection chambers. The first-layer chip is provided with a sample loading hole, and the sample loading hole communicates with the detection chambers through the microfluidic channel. The chip body further includes electrodes, and the electrodes are disposed within the detection chambers in one-to-one correspondence.

Abstract: A detection system for multi-index coagulation items, including a housing, a detection and heating module, a battery module, a touch display screen and a mainboard. The housing includes an upper housing and a lower housing, and is an enclosed space formed by the upper housing abutting the lower housing; the mainboard is disposed between the upper and the lower housing, and is arranged in the enclosed space; the touch display screen is disposed at the upper surface of upper housing, and is connected with the mainboard; the detection and heating module is disposed in the enclosed space, and is arranged at the front end of lower housing for connecting with the mainboard; the battery module is arranged at the bottom of terminal of lower housing, and is connected with the mainboard; and a detection inlet for inserting and placing a detection card is disposed at the front end of housing.

Abstract: Disclosed are a method for whole blood filtration and a filter membrane structure for whole blood filtration, specifically including the following steps: (1) a filter membrane structure made up of at least two filtration membranes sequentially stacked from top to bottom is selected, and subjected to hemagglutinin treatment for later use; (2) a whole blood sample is added to the filter membrane structure for filtration; and (3) the filtered serum or plasma is collected. The filter membrane structure is composed of at least two filtration membranes stacked from top to bottom, and the pore sizes of the filtration membranes stacked gradually decrease from top to bottom, and the areas of the same gradually increase or are equal to each other from top to bottom.

Abstract: A microfluidic detection chip for multi-channel rapid detection, including a chip body. A chip sampling port, a plurality of independent detection chambers, and a microfluidic channel are disposed on the chip body, and the chip sampling port is connected to the detection chambers by means of the microfluidic channel. The chip body further includes an electrode. The detection chambers are connected to the electrode. The microfluidic channel includes a main flow channel and a plurality of branching microfluidic channels. A tail end of the main flow channel is divided into the plurality of branching microfluidic channels, and the plurality of branching microfluidic channels are connected to the plurality of independent detection chambers in a one-to-one corresponding manner. And, the other end of the main flow channel is connected to the chip sampling port.