Abstract: This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3?UTR from Zea mays chlorophyll a/b binding protein gene. Some embodiments relate to a 3? UTR from a Zea mays chlorophyll a/b binding protein gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

Abstract: This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3?UTR from Zea mays chlorophyll a/b binding protein gene. Some embodiments relate to a 3? UTR from a Zea mays chlorophyll a/b binding protein gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

Abstract: This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3?UTR from Oryza sativa Ubiquitin-3 gene. Some embodiments relate to a 3? UTR from an Oryza sativa Ubiquitin-3 gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

Abstract: Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5?-UTRs, and/or 3?-UTRs, isolated from Zea mays.

Abstract: Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5?-UTRs, introns, and/or 3?-UTRs, isolated from Zea mays.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays metallothionein-like gene regulatory elements.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays chlorophyll a/b binding gene regulatory elements.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3?-UTRs, isolated from Brachypodium distachyon ubiquitin genes.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays GRMZM2G015295 gene regulatory elements.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays chlorophyll a/b binding gene regulatory elements.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays chlorophyll a/b binding gene regulatory elements.

Abstract: Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5?-UTRs, and/or 3?-UTRs, isolated from Zea mays.

Abstract: Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5?-UTRs, introns, and/or 3?-UTRs, isolated from Zea mays.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays metallothionein-like gene regulatory elements.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3?-UTRs, isolated from Brachypodium distachyon ubiquitin genes.

Abstract: The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

Abstract: The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

Abstract: The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

Abstract: The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

Abstract: The present invention relates to methods for the cryopreservation of transformed and non-transformed cells. Also provided by the subject invention are methods of recovering cells that have been cryopreserved. Cultures of cells that have been successfully recovered from cryopreservation are also provided.