Patents by Inventor Jeffrey BRODIN

Jeffrey BRODIN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230295739
    Abstract: Methods are provided for conducting an assay on a set of nucleic acid targets, including: combining a set of dual probes with a sample composition potentially comprising a set of nucleic acid targets to form a set of cleavable ternary nucleic acid complexes; releasing from the cleavable ternary nucleic acid complex a set of recognition element fragments comprising the recognition element sequence; combining the released recognition element fragments with an additional nucleic acid sequence from a set of additional nucleic acid sequences to create a circular nucleic acid, wherein the circular nucleic acid comprises a target-associated code and additional sequence elements; and performing a detection event to identify a set of detected codes of the target-associated codes.
    Type: Application
    Filed: January 5, 2023
    Publication date: September 21, 2023
    Inventors: Jeffrey BRODIN, Lorenzo BERTI, Brian EIDSON, Christian SCHLEGEL, Angela BLUM, Rachel SCHOWALTER, Ludovic VINCENT, Pieter VAN ROOYEN, Gavin STONE
  • Publication number: 20230287476
    Abstract: Degradable polyester bead are described comprising a plurality of transposome complexes immobilized to the surface thereof, wherein each transposome complex comprises a transposase bound to a first polynucleotide and a second polynucleotide, wherein the first polynucleotide comprises a 3? portion comprising a transposon end sequence and a tag, and the second polynucleotide comprises a 5? portion that is complementary to and hybridized to the transposon end sequence, and wherein the polyester bead has a melting point of from 50° C. to 65° C. Flow cells and methods related to these polyester beads are described. Also described herein are compositions comprising a bead and at least one nanoparticle and methods of use of such compositions comprising transposome complexes immobilized to nanoparticles.
    Type: Application
    Filed: January 6, 2023
    Publication date: September 14, 2023
    Applicant: Illumina, Inc.
    Inventors: Yir-Shyuan Wu, Tarun Kumar Khurana, Elisabet Rosàs-Canyelles, Fei Shen, Jeffrey Brodin, Lena Storms, Jeffrey S. Fisher
  • Publication number: 20230257801
    Abstract: Methods are provided for conducting a dual-probe assay on a set of nucleic acid targets, including: combining a set of dual probes with a sample composition potentially comprising a set of nucleic acid targets to form a set of cleavable ternary nucleic acid complexes; releasing from the cleavable ternary nucleic acid complex a set of recognition element fragments; hybridizing each of the set of released recognition element fragments to a coded oligonucleotide probe and using resulting hybridized released recognition elements as primers for copying the coded oligonucleotide probe to produce a sect of target-associated codes, wherein each of the coded oligonucleotide probes comprises a code from a set of codes, each code comprises at least one segment encoding one or more symbols that correspond to a sequence of one or more nucleotides; and performing a detection event to identify a set of detected codes of the target-associated codes.
    Type: Application
    Filed: January 5, 2023
    Publication date: August 17, 2023
    Inventors: Jeffrey BRODIN, Lorenzo BERTI, Brian EIDSON, Christian SCHLEGEL, Angela BLUM, Rachel SCHOWALTER, Ludovic VINCENT, Pieter VAN ROOYEN, Gavin STONE
  • Publication number: 20220356514
    Abstract: A method for detecting different analytes includes mixing different analytes with sensing probes, wherein at least some of the sensing probes are specific to respective ones of the analytes. The analytes respectively are captured by the sensing probes that are specific to those analytes. Fluorophores respectively are coupled to sensing probes that captured respective analytes. The sensing probes are mixed with beads, wherein the beads are specific to respective ones of the sensing probes, and wherein the beads include different codes identifying the analytes to which those sensing probes are specific. The sensing probes respectively are coupled to beads that are specific to those sensing probes. The beads are identified that are coupled to the sensing probes that captured analytes using at least fluorescence from the fluorophores coupled to those sensing probes. The analytes that are captured are identified.
    Type: Application
    Filed: October 12, 2020
    Publication date: November 10, 2022
    Applicants: ILLUMINA, INC., ILLUMINA SINGAPORE PTE. LTD., ILLUMINA CAMBRIDGE LIMITED
    Inventors: Sarah SHULTZABERGER, Jeffrey BRODIN, Yin Nah TEO, Suzanne ROHRBACK, Rebecca MACLEOD, Rigo PANTOJA, Allen ECKHARDT, Jeffrey FISHER, Xiangyuan YANG, Kaitlin PUGLIESE, Misha GOLYNSKIY, Xiaolin WU, Seth MCDONALD
  • Publication number: 20210187469
    Abstract: Provided is a nanoparticle including a scaffold, a single template site for bonding a template polynucleotide to the scaffold, and a plurality of accessory sites for bonding accessory oligonucleotides to the scaffold, wherein the scaffold is selected from one or more scaffold DNA molecules and one or more scaffold polypeptides, the single template site for bonding a template polynucleotide to the scaffold is selected from a covalent template bonding site and a noncovalent template bonding site and the plurality of accessory sites for bonding accessory oligonucleotides to the scaffold are selected from covalent accessory oligonucleotide bonding sites and noncovalent accessory oligonucleotide bonding sites. Also provided are methods of using the nanoparticle.
    Type: Application
    Filed: December 22, 2020
    Publication date: June 24, 2021
    Applicants: ILLUMINA, INC., ILLUMINA CAMBRIDGE LIMITED, ILLUMINA SINGAPORE PTE. LTD.
    Inventors: Wayne N. GEORGE, Jonathan Mark BOUTELL, Mathieu LESSARD-VIGER, Saurabh NIRANTAR, Pietro GATTI LAFRANCONI, Seth MCDONALD, Yin Nah TEO, Maria Rogert BACIGALUPO, Sergio PEISAJOVICH, Eric BRUSTAD, Michael HOWARD, Xiangyuan YANG, M. Shane Bowen, Alex NEMIROSKI, Jeffrey BRODIN, Ludovic VINCENT, Lorenzo BERTI, Andrew A. BROWN, Fei SHEN, Lena STORMS, Olivia BENICE