Abstract: A novel method of diluting the structures in the cell population, such that individual cells, dependent on the activity of the structures, become single measurement devices. This can be applied to all Bacterial Microcomparments (“BMCs”), organelles, and macromolecules, and could provide a universal method for the design of novel ones and understanding of the diverse structures. In one aspect the present invention provides A method of creating a bacterial strain with inducible and detectable carboxysomes. The method includes the steps of incorporating a labeled carbon-fixation enzyme into the genome of a bacterium; deleting all or a portion of the ccm operon from the bacterium; and reintroducing a ccm operon comprising an inducible promoter to create a ?ccm+ strain.

Abstract: A method for scheduling resources from a workforce pool, including the steps of updating human resource information regarding availability of each human resource, forecasting human resource workload for a specified time period, storing the human resource information and the forecasted human resource workload information into a central database, accessing the central database to schedule non-pooled human resources, determining understaffed time periods by comparing the forecasted human resource workload versus available resources from scheduling the non-pooled human resources and scheduling pooled human resources to cover the understaffed forecast.