Abstract: A system and method are presented that store graph data in a database as nodes, edges that link nodes, and detached profiles that contain properties that provide additional context for the nodes and edges. Computerized methods are described that provide for the dynamic creation of graph data based on the properties stored on the profiles related to each node, including the ability to predetermine which properties are used within a CSS-like language. Detached profiles allow a single profile, comprising a set of attribute-value pairs, to be associated with multiple database objects, or to survive the deletion of associated objects.

Abstract: A system and method are presented that store graph data in a database as nodes, edges that link nodes, and detached profiles that contain properties that provide additional context for the nodes and edges. Computerized methods are described that provide for the dynamic creation of graph data based on the properties stored on the profiles related to each node, including the ability to predetermine which properties are used within a CSS-like language. Detached profiles allow a single profile, comprising a set of attribute-value pairs, to be associated with multiple database objects, or to survive the deletion of associated objects.

Abstract: A system and method are presented that store graph data in a database as nodes, edges that link nodes, and profiles that contain properties that provide additional context for the nodes and edges. Computerized methods are described that provide for the dynamic creation of graph data based on the properties stored on the profiles related to each node, including the ability to predetermine which properties are used within a CSS-like language. Methods for visually focusing on a portion of a graph are also disclosed that allow a user to select one or more base nodes and limit the display of related nodes to those nodes within a defined degree of separation from the base node(s). The user can control the desired degree of separation, can select multiple base nodes, and can predetermine the base nodes to be used (and desired degree of separation for each) using a CSS-like language.

Abstract: A system and method are presented that store graph data in a database as nodes, edges that link nodes, and profiles that contain properties that provide additional context for the nodes and edges. Computerized methods are described that provide for the dynamic creation of graph data based on the properties stored on the profiles related to each node, including the ability to predetermine which properties are used within a CSS-like language. Methods for visually focusing on a portion of a graph are also disclosed that allow a user to select one or more base nodes and limit the display of related nodes to those nodes within a defined degree of separation from the base node(s). The user can control the desired degree of separation, can select multiple base nodes, and can predetermine the base nodes to be used (and desired degree of separation for each) using a CSS-like language.

Abstract: A three-dimensional microwell system that supports long term pluripotent cell culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured pluripotent cells remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel®-coated, tissue culture-treated polystyrene dishes. Microwell-cultured pluripotent cells maintain pluripotency, differentiating to each of the three embryonic germ layers. Pluripotent cell aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain pluripotent cell growth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct pluripotent cell differentiation by generating monodisperse EBs of a desired size and shape.

Abstract: The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.

Abstract: The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.

Abstract: The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.

Abstract: The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.

Abstract: A three-dimensional microwell system that supports long term pluripotent cell culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured pluripotent cells remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel®-coated, tissue culture-treated polystyrene dishes. Microwell-cultured pluripotent cells maintain pluripotency, differentiating to each of the three embryonic germ layers. Pluripotent cell aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain pluripotent cell growth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct pluripotent cell differentiation by generating monodisperse EBs of a desired size and shape.

Abstract: The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.

Abstract: The present invention relates to methods and structures for preparing stem cells for use in cryopreservation methods. Stem cell colonies are provided between first and second matrix portions and are exposed to a carbohydrate-containing cryoprotecting medium and a freezing medium. The methods of the invention yield cryopreserved cells that maintain cell viability and exhibit limited cell differentiation after freezing and thawing, facilitating storage, shipping and handling of embryonic stem cell stocks and lines for research and therapeutics.