Abstract: The subcellular localization of mRNA, and small RNA movement and trafficking of these molecules in an organism are important components of cellular and organismal regulation and communication. The next frontier for analysis of RNA will involve analysis at a single-molecule level, and at the subcellular level. This invention relates to methods and materials for sensitive detection of target molecules, particularly to methods and materials for binding or otherwise associating with target molecules and producing a signal for detection of, for example, spatial and/or temporal localization, and more particularly to methods and materials for the above using aptamers which bind to a chromophore or otherwise produce fluorescence or other detectable signal. This invention further relates to aptamers which bind to chromophores such as, for example, biliverdin or other related molecules, and also to such molecules which exhibit fluorescence or other detectable signals.

Abstract: A method of, apparatus for, or storage device medium having instructions for publishing a first notification within a data processing system where the data processing system comprises a first source generating the first notification, a first destination device, and a second destination device. The source may be a storage unit, a printer, a processing unit, or any other device which is a component of the data processing system. The destination device is typically a monitor, a workstation, a computer, a PC, or other input/output type workstation. The method comprises of having the first destination device subscribe to receive notifications from the first source. In addition, the second destination device subscribes to receive the notifications serially republished from the first destination device. Upon the publication of a notification from the first source to the first destination device, the first destination device will publish the notification to the second destination device.