Abstract: Systems and methods are disclosed to manipulate or normalize image of animals to a reference size and shape. Synthetically normalizing the image data to a reference size and shape allows machine learning models to automatically identify subject behaviors in a manner that is robust to changes in the size and shape of the subject. The systems and methods of the invention can be applied to drug or gene therapy classification, drug or gene therapy screening, disease study including early detection of the onset of a disease, toxicology research, side-effect study, learning and memory process study, anxiety study, and analysis in consumer behavior.