Abstract: Mechanical modifications to a spinal rod that will enable the rod to accept various coating technologies in such a way that the spinal construct's biomechanical performance is not compromised. These modifications preserve construct biomechanics in the presence of a coating and increase the bactericidal payload of an anti-infective spinal rod coating.

Abstract: Systems and methods for secure updating of allocations to user accounts are provided. In one aspect, a system includes one or more computer readable storage mediums having program instructions embodied therewith, and one or more processors configured to cause the system to identify an external institution associated with the future transfers, and initiate, based on the identified external institution, a proxy instance of a software application of the external institution to determine a set of endpoints and a set of the future transfers to the endpoints. The system is further configured to receive a request from a user to change at least one of the set of the endpoints and the set of the further transfers to the endpoints, and use the proxy instance, executing the requested change to at least one of the set of the endpoints or the set of the future transfers to the endpoints.

Abstract: A composition for amplifying a polynucleotide is provided that includes a substrate comprising a first region and a second region. A first plurality of capture primers is coupled to the first region of the substrate. A second plurality of capture primers is coupled to the second region of the substrate. The capture primers of the second plurality of capture primers are longer than the capture primers of the first plurality of capture primers. A first plurality of orthogonal capture primers are coupled to the first region of the substrate. A second plurality of orthogonal capture primers are coupled to the second region of the substrate. The orthogonal capture primers of the second plurality of orthogonal capture primers are shorter than the orthogonal capture primers of the first plurality of orthogonal capture primers.

Abstract: Provided is a method, including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments. Also provided is a method including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments.

Abstract: The present disclosure is generally directed to strategies for template capture and amplification during sequencing. In some examples, a solid support is used for template capture and amplification.

Abstract: Some embodiments provided herein include methods and compositions for the detection of target ligands on an array. In some embodiments, a capture probe specifically binds to a target ligand from a sample, the location of a bead comprising the capture probe in an array is determined, and the bead is decoded to identify the capture probe and the sample. In some embodiments, a barcode is indicative of a capture probe attached to a bead; and an index is indicative of a subpopulation of beads. In some embodiments, the barcode and the index are determined by sequencing.

Abstract: Provided is a method, including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments. Also provided is a method including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments.

Abstract: Some embodiments provided herein include methods and compositions for the detection of target ligands on an array. In some embodiments, a capture probe specifically binds to a target ligand from a sample, the location of a bead comprising the capture probe in an array is determined, and the bead is decoded to identify the capture probe and the sample. In some embodiments, a barcode is indicative of a capture probe attached to a bead; and an index is indicative of a subpopulation of beads. In some embodiments, the barcode and the index are determined by sequencing.

Abstract: A method for detecting different analytes includes mixing different analytes with sensing probes, wherein at least some of the sensing probes are specific to respective ones of the analytes. The analytes respectively are captured by the sensing probes that are specific to those analytes. Fluorophores respectively are coupled to sensing probes that captured respective analytes. The sensing probes are mixed with beads, wherein the beads are specific to respective ones of the sensing probes, and wherein the beads include different codes identifying the analytes to which those sensing probes are specific. The sensing probes respectively are coupled to beads that are specific to those sensing probes. The beads are identified that are coupled to the sensing probes that captured analytes using at least fluorescence from the fluorophores coupled to those sensing probes. The analytes that are captured are identified.

Abstract: An image sensor structure includes an image layer having an array of light detectors disposed therein. A device stack is disposed over the image layer. An array of light guides is disposed in the device stack. Each light guide is associated with a light detector. An array of nanowells is disposed over the device stack. Each nanowell is associated with a first light guide of the array of light guides. A first primer set is disposed throughout a first well region of each nanowell. A second primer set is disposed throughout a second well region of each nanowell. The second well region is adjacent the first well region. The first and second primer sets are operable to attach a forward strand cluster of forward polynucleotide strands in the first well region and a reverse strand cluster of reverse polynucleotide strands in the second well region.

Abstract: A composition for amplifying a polynucleotide is provided that includes a substrate comprising a first region and a second region. A first plurality of capture primers is coupled to the first region of the substrate. A second plurality of capture primers is coupled to the second region of the substrate. The capture primers of the second plurality of capture primers are longer than the capture primers of the first plurality of capture primers. A first plurality of orthogonal capture primers are coupled to the first region of the substrate. A second plurality of orthogonal capture primers are coupled to the second region of the substrate. The orthogonal capture primers of the second plurality of orthogonal capture primers are shorter than the orthogonal capture primers of the first plurality of orthogonal capture primers.

Abstract: Provided is a method, including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments. Also provided is a method including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments.

Abstract: Some embodiments provided herein include methods and compositions for the detection of target ligands on an array. In some embodiments, a capture probe specifically binds to a target ligand from a sample, the location of a bead comprising the capture probe in an array is determined, and the bead is decoded to identify the capture probe and the sample. In some embodiments, a barcode is indicative of a capture probe attached to a bead; and an index is indicative of a subpopulation of beads. In some embodiments, the barcode and the index are determined by sequencing.

Abstract: A video stream demultiplxer receives video streams comprising a selected current content channel and one or more adjacent content channels. Time stamp management is concurrently performed on the adjacent content channels while decoding the selected current content channel. Timing information such as Decoding Time Stamp (DTS) and Presentation Time Stamp (PTS) values, and/or random access points (RAPs) may be determined for pictures stored for the selected current content channel and the adjacent content channels. The determined timing information is utilized to determine a Program Clock Reference (PCR) value for each of the selected current channel and the adjacent channels for channel monitoring. A timebase, derived from a PCR rate that is determined based on the determined PCR value, is determined for decoding the selected current content channel. An adjacent content channel, which is primed during decoding of the selected current content channel, may be directly decoded for display if selected.

Abstract: Systems and methods are provided for evaluating composition of a first file representing a document to be evaluated. An evaluation method transforms the first file to a second file. The second file includes a plurality of objects corresponding to the composition of the first file. The evaluation method also determines parameters based on the plurality of objects; evaluates the parameters based on a plurality of composition rules provided by a rule engine; generates evaluation findings and stores the evaluation findings; and generates an evaluation conclusion based on the evaluation findings. The evaluation conclusion indicates compliance of the document according to the composition rules.

Abstract: A video stream demultiplxer receives video streams comprising a selected current content channel and one or more adjacent content channels. Time stamp management is concurrently performed on the adjacent content channels while decoding the selected current content channel. Timing information such as Decoding Time Stamp (DTS) and Presentation Time Stamp (PTS) values, and/or random access points (RAPs) may be determined for pictures stored for the selected current content channel and the adjacent content channels. The determined timing information is utilized to determine a Program Clock Reference (PCR) value for each of the selected current channel and the adjacent channels for channel monitoring. A timebase, derived from a PCR rate that is determined based on the determined PCR value, is determined for decoding the selected current content channel. An adjacent content channel, which is primed during decoding of the selected current content channel, may be directly decoded for display if selected.

Abstract: In some embodiments, flat electrical contact pads may be fabricated along a routed edge face of a printed wiring board (PWB). Some embodiments of the edge pads may, for example, be perpendicular to a plane of the printed wiring board. In some embodiments, the edge pads may be of a specified length and/or width. Some embodiments of the edge pads may, for example, have a surface finish suitable for soldering and/or for direct contact interconnections. In some embodiments, the edge pads may, for example, be configured for electrical connection to an adjoining device (e.g., PWB) with mating pads to form a tiled array configuration of interconnected devices (e.g., PWBs).

Abstract: A video stream demultiplexer receives video streams including a selected current content channel and one or more adjacent content channels. Time stamp management may be concurrently performed on the adjacent content channels while decoding the selected current content channel. Timing information such as Decoding Time Stamp, Presentation Time Stamp values, or random access points may be determined for pictures of the selected current content channel and the adjacent content channels. The determined timing information may be utilized to determine a Program Clock Reference value for the selected current channel and the adjacent channels. A timebase, derived from a PCR rate, may be determined for decoding the selected current content channel. An adjacent content channel, which is primed during decoding of the selected current content channel, may be directly decoded for display if selected.

Abstract: An IP network includes a central entity and at least one customer premises equipment (CPE) device. The central entity generates a program clock reference (PCR) clock and provides audio-visual packets to a CPE based on the PCR clock. The CPE sets a first clock based on the PCR clock for decoding operations. The CPE sets a second clock that is independent from the first clock for audio and video output operations. For example, the CPE can process the audio-visual packets using the second clock.

Abstract: The presently disclosed device provides a method and means for ensuring that containers of all types and sizes are vented or purged to atmospheric or environmental conditions upon the interior or exterior of the container reaching a critical temperature pressure, or humidity. Specifically, the presently disclosed invention integrates shape memory polymer (SMP) based, thermally activated fasteners into the venting systems. The result is a venting system that increases munitions safety without compromising the venting effectiveness, structural integrity, or the required bullet/fragment impact resistance requirements of the system.