Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3?—OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3?-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3?-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3?-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

Abstract: An ultrasound transducer positioning apparatus and method that are capable of securing an ultrasound transducer in a desired position on a patient's body are disclosed. In some implementations, pressure exerted by the apparatus on the ultrasound transducer influences the angle at which the ultrasound energy is delivered to the patient's body.

Abstract: An ultrasound transducer positioning apparatus and method that are capable of securing an ultrasound transducer in a desired position on a patient's body are disclosed. In some implementations, pressure exerted by the apparatus on the ultrasound transducer influences the angle at which the ultrasound energy is delivered to the patient's body.

Abstract: The invention provides mutants of DNA polymerases having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases.

Abstract: Methods for designing and developing models for simulating the behavior of clock signals and in particular those generated by phase-locked loop (PLL) circuits are provided. The clock period of a phase-locked loop circuit's variable frequency oscillator signal may be modeled by combining the inverse of the oscillator frequency rounded up to the simulation time scale with the inverse rounded down to the simulation time scale. The variable frequency oscillator signal may further be synchronized with a reference clock signal at a rate determined by the relationship between the reference clock signal and the variable frequency oscillator signal. A parameter may indicate a target range for the deviation between the two signals and a runtime monitor may be used together with the parameter setting to decide whether synchronization is required and make the appropriate adjustments.

Abstract: The present invention provides mutants of DNA polymerases having an increased rate of incorporation of nucleotides into nucleic acids undergoing polymerization and having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for fast PCR applications, for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases, and for fast PCR amplification of samples containing DNA polymerase inhibitors. In exemplary embodiments, the mutants are mutants of Taq DNA polymerase.

Abstract: A circuit designer may use computer-aided design (CAD) tools to implement an integrated circuit design. The CAD tools may include auto-pipelining capabilities to improve the performance of the integrated circuit design. Auto-pipelining may modify the number of pipeline registers in a path within a given range. A description of the integrated circuit design may include different implementation alternatives of a path each having a different number of pipeline registers, and the CAD tools may select one of these implementation alternatives. The CAD tools may further evaluate the performance of a particular implementation alternative and iteratively select a different implementation alternative until a given objective is met. The CAD tool may update a test environment according to the selected implementation alternative once the objective is met and validate the selected implementation alternative using the updated test environment.

Abstract: The invention provides mutants of DNA polymerases having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases.

Abstract: A circuit designer may use computer-aided design (CAD) tools to implement an integrated circuit design. The CAD tools may include auto-pipelining capabilities to improve the performance of the integrated circuit design. Auto-pipelining may modify the number of pipeline registers in a path within a given range. A description of the integrated circuit design may include different implementation alternatives of a path each having a different number of pipeline registers, and the CAD tools may select one of these implementation alternatives. The CAD tools may further evaluate the performance of a particular implementation alternative and iteratively select a different implementation alternative until a given objective is met. The CAD tool may update a test environment according to the selected implementation alternative once the objective is met and validate the selected implementation alternative using the updated test environment.

Abstract: The present invention provides mutants of DNA polymerases having an increased rate of incorporation of nucleotides into nucleic acids undergoing polymerization and having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for fast PCR applications, for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases, and for fast PCR amplification of samples containing DNA polymerase inhibitors. In exemplary embodiments, the mutants are mutants of Taq DNA polymerase.

Abstract: The present invention provides methods, compositions, and kits for storing and enhancing the activity of thermostable polymerases. The methods comprise mixing a thermostable polymerase with at least one zwitterionic detergent or non-detergent surfactant. Compositions and kits for performing the process according to the invention are also provided.

Abstract: A computer array (10) has a plurality of computers (12) for accomplishing a larger task that is divided into smaller tasks, each of the smaller tasks being assigned to one or more of the computers (12). Each of the computers (12) may be configured for specific functions and individual input/output circuits (26) associated with exterior computers (12) are specifically adapted for particular input/output functions. An example of 24 computers (12) arranged in the computer array (10) has a centralized computational core (34) with the computers (12) nearer the edge of the die (14) being configured for input and/or output. Mechanisms are described for communications between computers (12) and the outside environment.

Abstract: The present invention relates to evaluating the effect of physiological conditions on the occurrence of ventricular fibrillation, identifying strategies for treatment or prevention of ventricular fibrillation or ventricular tachycardia, and evaluating a subject for induction of ventricular fibrillation from a condition of ventricular tachycardia.

Abstract: A microprocessor system in which an array of processors communicates more efficiently through the use of a worker mode function. Processors that are not currently executing code remain in an inactive but alert state until a task is sent to them by an adjacent processor. Processors can also be programmed to temporarily suspend a task to check for incoming tasks or messages.

Abstract: A computer array (10) has a plurality of computers (12). The computers (12) communicate with each other asynchronously, and the computers (12) themselves operate in a generally asynchronous manner internally. When one computer (12) attempts to communicate with another it goes to sleep until the other computer (12) is ready to complete the transaction, thereby saving power and reducing heat production. The sleeping computer (12) can be awaiting data or instructions (12). In the case of instructions, the sleeping computer (12) can be waiting to store the instructions or to immediately execute the instructions. In the later case, the instructions are placed in an instruction register (30a) when they are received and executed therefrom, without first placing the instructions first into memory. The instructions can include a micro-loop (100) which is capable of performing a series of operations repeatedly.

Abstract: A computer array (10) has a plurality of computers (12). The computers (12) communicate with each other asynchronously, and the computers (12) themselves operate in a generally asynchronous manner internally. When one computer (12) attempts to communicate with another it goes to sleep until the other computer (12) is ready to complete the transaction, thereby saving power and reducing heat production. The sleeping computer (12) can be awaiting data or instructions (12). In the case of instructions, the sleeping computer (12) can be waiting to store the instructions or to immediately execute the instructions. In the later case, the instructions are placed in an instruction register (30a) when they are received and executed therefrom, without first placing the instructions first into memory. The instructions can include a micro-loop (100) which is capable of performing a series of operations repeatedly.