Abstract: A chromatography system may include: a first solvent supply operatively connected to a chromatography column; a second solvent supply operatively connected to the chromatography column; a controller operatively connected to the first and second solvent supplies for delivering a flow of a solvent system to an inlet for the chromatography column in accord with a predetermined solvent gradient, wherein the flow of the solvent system through the chromatography column will separate components from a sample composition; and a collection apparatus operatively connected to an outlet from the chromatography column for collecting designated portions of the solvent system flow exiting the column. The controller may determine a target sample mass as a function of chromatography column parameters.

Abstract: Provided are methods and apparatus for determining conditions for performing chromatography, particularly flash chromatography. The methods may be utilized for separating compounds in a sample in a column chromatography system. The method includes determining at least two retention factors corresponding to the TLC performance of a target compound and an adjacent compound and defining a sample loading amount and a solvent gradient profile using at least two solvents that will result in differential elution of the target and adjacent compounds from a chromatography column. The chromatography system and loading parameters may be selected to provide satisfactory separation of the target compound from adjacent compounds.

Abstract: Provided are methods and apparatus for determining conditions for performing chromatography, particularly flash chromatography. The methods may be utilized for separating compounds in a sample in a column chromatography system. The method includes determining at least two retention factors corresponding to the TLC performance of a target compound and an adjacent compound and defining a sample loading amount and a solvent gradient profile using at least two solvents that will result in differential elution of the target and adjacent compounds from a chromatography column. The chromatography system and loading parameters may be selected to provide satisfactory separation of the target compound from adjacent compounds.

Abstract: Provided are methods and apparatus for determining conditions for performing chromatography, particularly flash chromatography. The methods may be utilized for separating compounds in a sample in a column chromatography system. The method includes determining at least two retention factors corresponding to the TLC performance of a target compound and an adjacent compound and defining a sample loading amount and a solvent gradient profile using at least two solvents that will result in differential elution of the target and adjacent compounds from a chromatography column. The chromatography system and loading parameters may be selected to provide satisfactory separation of the target compound from adjacent compounds.

Abstract: Provided are methods and apparatus for determining conditions for performing chromatography, particularly flash chromatography. The methods may be utilized for separating compounds in a sample in a column chromatography system. The method includes determining at least two retention factors corresponding to the TLC performance of a target compound and an adjacent compound and defining a sample loading amount and a solvent gradient profile using at least two solvents that will result in differential elution of the target and adjacent compounds from a chromatography column. The chromatography system and loading parameters may be selected to provide satisfactory separation of the target compound from adjacent compounds.

Abstract: A method of processing a sample comprising introducing a sample into a flow-through device containing a porous solid media therein, and thereafter subjecting the device to microwave energy.

Abstract: A method of processing a sample comprising introducing a sample into a flow-through device containing a porous solid media therein, and thereafter subjecting the device to microwave energy.

Abstract: Apparatus for chromatography, featuring a composite chromatography column including a tubular member having an inlet end and an outlet end, where the tubular member includes an innermost first layer comprised of a first material and asecond layer comprised of a second material. The chromatography apparatus can also include a first intermediate layer formed from a third material, where at least a portion of the first intermediate layer is disposed between the innermost first layer and the second layer. A second intermediate layer formed from a fourth material, can further be included in the chromatography apparatus, where at least a portion of the second intermediate layer is disposed between the innermost first layer and the second layer. A portion of the second intermediate layer can be disposed between the second layer and the first intermediate layer.

Abstract: An apparatus for use in liquid chromatography involves a column filled with media bounded axially by first and second porous plates. The column has an inlet end with an inflow region between the inlet end and the first porous plate. The column also has an outlet end with an outflow region between the outlet end and the second porous plate. The inflow region may be substantially longer than the outflow region. The porous plates may be moveable under pressure so as to compress the media.

Abstract: A method of processing a sample comprising introducing a sample into a flow-through device containing a porous solid media therein, and thereafter subjecting the device to microwave energy.

Abstract: Apparatus for chromatography, featuring a composite chromatography column including a tubular member having an inlet end and an outlet end, where the tubular member includes an innermost first layer comprised of a first material and a second layer comprised of a second material. The chromatography apparatus can also include a first intermediate layer formed from a third material, where at least a portion of the first intermediate layer is disposed between the innermost first layer and the second layer. A second intermediate layer formed from a fourth material, can further be included in the chromatography apparatus, where at least a portion of the second intermediate layer is disposed between the innermost first layer and the second layer. A portion of the second intermediate layer can be disposed between the second layer and the first intermediate layer.

Abstract: Apparatus for chromatography, featuring a composite chromatography column including a tubular member having an inlet end and an outlet end, where the tubular member includes an innermost first layer comprised of a first material and a second layer comprised of a second material. The chromatography apparatus can also include a first intermediate layer formed from a third material, where at least a portion of the first intermediate layer is disposed between the innermost first layer and the second layer. A second intermediate layer formed from a fourth material, can further be included in the chromatography apparatus, where at least a portion of the second intermediate layer is disposed between the innermost first layer and the second layer. A portion of the second intermediate layer can be disposed between the second layer and the first intermediate layer.

Abstract: Techniques for chromatography, including a method for introducing a sample into a chromatography column having the following steps. A liquid reaction mixture is provided including a sample dissolved in a solvent. A chromatography sample module including a separation media is at least partially immersed in the liquid reaction mixture, such that the liquid reaction mixture is drawn into the sample module by capillary action. The sample module including the sample is inserted into a chromatography column.

Abstract: A method of processing a sample comprising introducing a sample into a flow-through device containing a porous solid media therein, and thereafter subjecting the device to microwave energy. Also disclosed is a flow through device including solid porous media therein and attached active components.

Abstract: A method of processing a sample comprising introducing a sample into a flow-through device containing a porous solid media therein, and thereafter subjecting the device to microwave energy. Also disclosed is a flow through device including solid porous media therein and attached active components.

Abstract: A method of processing a sample comprising introducing a sample into a flow-through device containing a porous solid media therein, and thereafter subjecting the device to microwave energy.

Abstract: A device for transferring small drops of liquid from one or more liquid holders to one or more liquid destinations is disclosed. The device includes a support member and a plurality of fluid transfer members extending from the support member. Each fluid transfer member includes a flexible liquid transfer end.

Abstract: An apparatus for use in liquid chromatography involves a column filled with media bounded axially by first and second porous plates. The column has an inlet end with an inflow region between the inlet end and the first porous plate. The column also has an outlet end with an outflow region between the outlet end and the second porous plate. The inflow region may be substantially longer than the outflow region. The porous plates may be moveable under pressure so as to compress the media.

Abstract: An apparatus for use in liquid chromatography involves a column filled with media bounded axially by at least one porous member. The column has an inlet end with an inflow region between the inlet and a porous member. The inflow region may be sufficiently long so that a chromatography sample module may be disposed therein. The column may be sufficiently long so that a chromatography sample module may be disposed therein. The column may also have an outlet end with an outflow region between the outlet end and a second porous member. The inflow region may be substantially longer than the outflow region. The porous members may be moveable relative to the column.

Abstract: A chromatography column includes a tubular member with an inlet end and a slidable porous member that bounds a chromatography media. The porous member is spaced sufficiently from the inlet end to define a receiving region.