Abstract: Embodiments of the invention utilizes advanced detection methodologies, such as the lab-on-a-chip (LOC) technology, as a cost-effective, efficient, ultra-sensitive rapid method for diagnosing Acute Myocardial Infarction (AMI) in human subjects. In certain aspects, multiple biomarkers of AMI are concurrently detected and measured in serum and saliva to provide a more efficient, sensitive and accurate diagnosis of AMI.

Abstract: Embodiments of the invention include methods by which cardiac biomarkers are assigned an index (cardiovascular biomarker index-cardiobioindex, CBI) as a means to describe the utility of each biomarker, or combination of biomarkers for risk evaluation, diagnosis or prognosis of cardiovascular disease status.

Abstract: A rapid, specific, serological method for identification of microorganisms is carried out by immunizing a warm-blooded mammal with a killed microorganism to obtain an antisera, conjugating the antisera with an enzyme marker, bonding a microorganism to a solid support, adding the enzyme-conjugated antisera to the bonded microorganism in the presence of a color reactant and identifying the microorganism from the color reaction. Preferably, immunizing is carried out with two injections of about 10.sup.9 killed microorganisms each with the second injection being about 14 to 21 days after the first, and recovering the antisera about 14 to 21 days after the second injection.