Abstract: The disclosure relates to novel methods, compositions, and genetically modified microorganisms for extracting and/or isolating bioproducts from microorganisms having recalcitrant cell walls. In some aspects, the disclosure relates to solvent-free methods of extracting and/or isolating bioproducts. The disclosure further relates to bioproducts having less than 10 ppm of a solvent.

Abstract: The present application provides materials and methods for treating hemoglobinopathies. More specifically, the application provides methods for producing progenitor cells that are genetically modified via genome editing to increase the production of fetal hemoglobin (HbF), as well as modified progenitor cells (including, for example, CD34+ human hematopoietic stem cells) producing increased levels of HbF, and methods of using such cells for treating hemoglobinopathies such as sickle cell anemia and ?-thalassemia.

Abstract: The present application provides materials and methods for treating hemoglobinopathies. More specifically, the application provides methods for producing progenitor cells that are genetically modified via genome editing to increase the production of fetal hemoglobin (HbF), as well as modified progenitor cells (including, for example, CD34+ human hematopoietic stem cells) producing increased levels of HbF, and methods of using such cells for treating hemoglobinopathies such as sickle cell anemia and ?-thalassemia.

Abstract: The present application provides materials and methods for treating a patient with Alpha-1 antitrypsin deficiency (AATD) both ex vivo and in vivo. In addition, the present application provides materials and methods for editing the SERPINA1 gene in a cell by genome editing.

Abstract: Systems and methods for map simulation testing are provided. A method includes receiving, by a computer-implemented system, information associated with an operational design domain (ODD); generating, by the computer-implemented system based at least in part on the information associated with the ODD, a plurality of map test cases for a map representative of a geographical area in the ODD, where each map test case of the plurality of map test cases includes one of a plurality of paths in the geographical area; evaluating a vehicle planning process in navigating through one or more of the plurality of paths corresponding to one or more of the plurality of map test cases; and determining a metric for the map based on the evaluating.

Abstract: The present application provides materials and methods for treating a patient with Alpha-1 antitrypsin deficiency (AATD) both ex vivo and in vivo. In addition, the present application provides materials and methods for editing the SERPINA1 gene in a cell by genome editing.

Abstract: A backplane for driving a display includes a two-dimensional array of pixel drive circuits, organized as a plurality of rows and a plurality of columns. The backplane has at least one shift register addressing assembly that includes a shift register chain formed of a plurality of controlling shift registers serially connected with, and separated by, equal sized groups of non-controlling shift registers. Each controlling shift register controls a different one of a plurality of word lines that each connect with pixel drive circuits of one row. The backplane also includes a plurality of bit lines that each connect with pixel drive circuits of one column. A shift register data sequence is input to a first one of the plurality of controlling shift registers and propagates through the shift register chain to control the plurality of word lines to load display values from the bit lines into the pixel drive circuits.

Abstract: The disclosed technology provides solutions for detecting and localizing road signage, such as speed limit signs. A process of the disclosed technology can include steps for receiving a first set of sensor data comprising a signage feature, wherein the first set of sensor data is associated with a first location, receiving a second set of sensor data comprising the signage feature, wherein the second set of sensor data is associated with a second location, and determining a location of the signage feature based on the first location and the second location. Systems and machine-readable media are also provided.

Abstract: A backplane for driving a display includes a two-dimensional array of pixel drive circuits, organized as a plurality of rows and a plurality of columns. The backplane has at least one shift register addressing assembly that includes a shift register chain formed of a plurality of controlling shift registers serially connected with, and separated by, equal sized groups of non-controlling shift registers. Each controlling shift register controls a different one of a plurality of word lines that each connect with pixel drive circuits of one row. The backplane also includes a plurality of bit lines that each connect with pixel drive circuits of one column. A shift register data sequence is input to a first one of the plurality of controlling shift registers and propagates through the shift register chain to control the plurality of word lines to load display values from the bit lines into the pixel drive circuits.

Abstract: The disclosed technology provides solutions for detecting and localizing road signage, such as speed limit signs. A process of the disclosed technology can include steps for receiving a first set of sensor data comprising a signage feature, wherein the first set of sensor data is associated with a first location, receiving a second set of sensor data comprising the signage feature, wherein the second set of sensor data is associated with a second location, and determining a location of the signage feature based on the first location and the second location. Systems and machine-readable media are also provided.

Abstract: A method for providing gray scale for an array of current emissive devices sensitive to the current level driving them, such as an LEDs, is disclosed. Multiple current sources are operated in parallel for each emissive device with the on/off state of each of the multiple current source controlled by a memory circuit. Means for pulse width modulating the array of current sensitive devices to enable greater control over gray scale is presented.

Abstract: Methods, systems, and storage media for issuing and verifying a secondary cryptographic photo ID are disclosed. Exemplary implementations may: capture, using a scanning component of a printing device, a primary digital image of a primary photo ID; recognize, using a processing component of the printing device, alphanumeric information in the captured primary digital image; encrypt, using the processing component of the printing device, the recognized alphanumeric information; generate, using the processing component of the printing device, a digital watermark comprising the encrypted recognized alphanumeric information; recognize, using the processing component of the printing device, a photograph in the primary digital image; generate, using the processing component of the printing device, a secondary digital image comprising the recognized photograph and the generated digital watermark; and print, using a printing component of the printing device, a secondary photo ID comprising the secondary digital image.

Abstract: Methods, systems, and storage media for issuing and verifying a secondary cryptographic photo ID are disclosed. Exemplary implementations may: capture, using a scanning component of a printing device, a primary digital image of a primary photo ID; recognize, using a processing component of the printing device, alphanumeric information in the captured primary digital image; encrypt, using the processing component of the printing device, the recognized alphanumeric information; generate, using the processing component of the printing device, a digital watermark comprising the encrypted recognized alphanumeric information; recognize, using the processing component of the printing device, a photograph in the primary digital image; generate, using the processing component of the printing device, a secondary digital image comprising the recognized photograph and the generated digital watermark; and print, using a printing component of the printing device, a secondary photo ID comprising the secondary digital image.

Abstract: The present application provides materials and methods for treating a patient with Alpha-1 antitrypsin deficiency (AATD) both ex vivo and in vivo. In addition, the present application provides materials and methods for editing the SERPINA1 gene in a cell by genome editing.

Abstract: The present application provides materials and methods for treating hemoglobinopathies. More specifically, the application provides methods for producing progenitor cells that are genetically modified via genome editing to increase the production of fetal hemoglobin (HbF), as well as modified progenitor cells (including, for example, CD34+ human hematopoietic stem cells) producing increased levels of HbF, and methods of using such cells for treating hemoglobinopathies such as sickle cell anemia and ?-thalassemia.

Abstract: A capacitive touch sensor has a clear top plastic film and graphics or other designs disposed on its reverse side. Only the bare plastic substrate is exposed to the environment and user wear. Non-conductive color inks are used in printing the graphics so that any printed capacitive sensors in contact behind will not be short circuited or desensitized. A silk-screened base color layer with a print-free margin left all around its edges allows a later application of adhesive to grip a more durable surface underneath. Capacitive touch sensors are deposited on a bottom plastic film with silvered conductive inks. The printed capacitive sensors on film are fastened with a silk-screen applied liquid water-based adhesive and face the graphics printed side of the first plastic film. A final overcoat of adhesive is applied to the bare backside of the bottom plastic film for final assembly to a frame or chassis.

Abstract: An interactive device has a plastic case for a mobile phone has graphics printed on the reverse side of a clear plastic film using non-conductive color inks. Electronic capacitive touch sensors are patterned and printed with conductive inks on a second plastic film in positions corresponding to individual graphic objects. A light guide layer is placed immediately beneath or above the pattern of printed electronic capacitive touch sensors. Diffusers on the top surface of the light guide layer catch light propagating and reflecting inside by refraction redirect it up to the user's eyes through openings in the non-conductive color ink graphics. The phone itself, a micro-controller, software, and associated electronic components mounted on a circuit board are used to control the lights sent into the light guide layer from its edges, and they control the response to touches being sensed by the touch sensors.

Abstract: A capacitive touch sensor has a clear top plastic film and graphics or other designs disposed on its reverse side. Only the bare plastic substrate is exposed to the environment and user wear. Non-conductive color inks are used in printing the graphics so that any printed capacitive sensors in contact behind will not be short circuited or desensitized. A silk-screened base color layer with a print-free margin left all around its edges allows a later application of adhesive to grip a more durable surface underneath. Capacitive touch sensors are deposited on a bottom plastic film with silvered conductive inks. The printed capacitive sensors on film are fastened with a silk-screen applied liquid water-based adhesive and face the graphics printed side of the first plastic film. A final overcoat of adhesive is applied to the bare backside of the bottom plastic film for final assembly to a frame or chassis.