Abstract: In this application is described the expression and purification of a recombinant Plasmodium falciparum (FVO) MSP-142. The method of the present invention produces a highly purified protein that retains folding and disulfide bridging of the native molecule. The recombinant MSP-142 is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.

Abstract: In this application is the expression and purification of a recombinant Plasmodium falciparum (3D7) MSP-142. The method of the present invention produces a highly purified protein which retains folding and disulfide bridging of the native molecule. The recombinant MSP-142 is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.

Abstract: In this application is described the expression and purification of a recombinant Plasmodium falciparum (3D7) LSA-NRC polypeptide. The method of the present invention produces a highly purified polypeptide which is useful as a vaccine and as a diagnostic reagent.

Abstract: This application is the expression and purification of a recombinant Plasmodium falciparum (3D7) MSP-142. The method of the present invention produces a highly purified protein which retains folding and disulfide bridging of the native molecule. The recombinant MSP-142 is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.

Abstract: The present invention is based in the fields of molecular genetics and recombinant protein expression. The invention features compositions and methods for increasing the expression levels of proteins expressed in non-natural hosts. Increased expression levels are achieved by expression of a translationally-harmonized nucleic acid that is created by substituting certain codons having low or intermediate usage frequency in the natural host with synonymous codons that have low or intermediate usage frequency in the non-natural host.

Abstract: The present invention provides a method for modifying a wild type nucleic acid sequence encoding a polypeptide to enhance expression and accumulation of the polypeptide in the host cell by harmonizing synonymous codon usage frequency between the foreign DNA and the host cell DNA. This can be done by substituting codons in the foreign coding sequence with codons of similar usage frequency from the host DNA/RNA which code for the same amino acid. The present invention also provides novel synthetic nucleic acid sequences prepared by the method of the invention.

Abstract: In this application is described the expression and purification of a recombinant Plasmodium falciparum (FVO) MSP-142. The method of the present invention produces a highly purified protein that retains folding and disulfide bridging of the native molecule. The recombinant MSP-142 is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.

Abstract: In this application is the expression and purification of a recombinant Plasmodium falciparum (3D7) MSP-142. The method of the present invention produces a highly purified protein which retains folding and disulfide bridging of the native molecule. The recombinant MSP-142 is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.

Abstract: In this application is the expression and purification of a recombinant Plasmodium falciparum (3D7) MSP-142. The method of the present invention produces a highly purified protein which retains folding and disulfide bridging of the native molecule. The recombinant MSP-142 is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.

Abstract: An array of micromachined mirrors are arranged on a first substrate at the intersections of input and output optical paths and oriented at approximately forty-five degrees to the paths. An array of split-electrodes are arranged on a second substrate above the respective mirrors. Each split electrode includes a first electrode configured to apply an electrostatic force that rotates the mirror approximately ninety degrees into one of the input optical paths to deflect the optical signal along one of the output optical paths, and a second electrode configured to apply an electrostatic force that maintains the mirror position. Stability may be improved by using the first and second electrodes in combination to first actuate the mirror and then balance the forces on the mirror to maintain its position. Reproducibly accurate positioning of the mirrors requires either the use of active positioning control or of mechanical stops.

Abstract: A computer integrated event management system is disclosed that coordinates the entire process of event planning and implementation using specialized software combined with a microcomputer network which includes a network file server, CAD/DATA station and DATA stations where facility personnel are able to efficiently plan and implement events. The system includes a fully relational database and a CAD module which are connected together using a third module such that, whenever a piece of information is changed in one place, it is automatically changed everywhere it is stored in the database. The connecting module permits the database and the CAD module to communicate by translating information from a database format into a graphic format readable by CAD software packages as well as translating the graphic information back into the database information stored in the database.

Abstract: An automated, computer-driven amino acid indexer for peptide synthesis uses programmed computer, a circuit board controller, and a combination of microtiter sample well trays, light emitting diodes to illuminate each sample well, and circuitry to control the illumination of the diodes. The apparatus simplifies technical difficulties present in large-scale laboratory syntheses of peptides by substantially reducing the time required for dispensing amino acids into sample trays and reducing the occurrence of error in the process to negligible levels in typical syntheses. A programmed, automated technique for synthesizing peptides is also provided.

Abstract: Apparatus for making decorative bows from lengths of ribbon wherein the apparatus ribbon is fed and wrapped into a plurality of loops which are punched at the center, trimmed to make one or more integral tails and stapled to a card.

Abstract: A decorative bow and method of making the same wherein the bow is formed from one or more continuous lengths of ribbon that in manufacturing is trimmed, folded, punched, and stapled to a card with the trailing edges cut and which upon fanning forms a bow having one or more integral legs that make up the tail.

Abstract: In a cooperative collision avoidance system the detected reply signals to interrogation probe signals are correlated by a digital circuit that processes all the reply signals identifying targets over non-targets ("fruit"). The correlator is capable of detecting all targets in any desired range during a given correlation period and is provided with means to reduce the effect of bridging or straddling adjacent bins in the correlator to minimize thereby the duplication of detected replies which would overload the following processor and cause loss of desired replies.

Abstract: In a cooperative collision avoidance system for aircraft, an altitude encoding system serves as a discriminant to insure orderly sequence of data exchange between cooperative aircraft, particularly, in a dense traffic environment. The system includes means for generating pairs of pulse-interval coded signals spaced to represent altitude addresses from interrogator to responder aircraft. Responder aircraft reply with a single pulse to such an altitude address only if the responder aircraft is at the altitude corresponding to the address.