Abstract: Compositions and methods, systems, and kits for detecting and quantifying variations in numbers of molecules, particularly variations in gene dosage, e.g., due to gene duplication, or to variations from the normal euploid complement of chromosomes, e.g., trisomy of one or more chromosomes that are normally found in diploid pairs, without digital sequencing.

Abstract: New or improved lead acid batteries with vapor pressure barriers and/or improved battery separators, as well as systems, vehicles, and/or methods of manufacture and/or use thereof are disclosed herein. In at least select embodiments, the instant disclosure provides new or improved lead acid batteries with a vapor pressure barrier. In at least select embodiments, the instant disclosure provides new or improved lead acid battery vapor pressure barriers along with new or improved battery separators, and/or methods of manufacture and/or use thereof. In at least select embodiments, the instant disclosure provides a new or improved lead acid battery with a vapor pressure barrier that reduces the water loss from the battery. In at least select embodiments, a method of reducing the water loss of a lead acid battery may include providing a vapor pressure barrier, such as a layer of oil, inside the lead acid battery along with an improved battery separator.

Abstract: Methods, systems, computer programs and computer readable media comprising instructions for processing images and for analyzing samples are described, wherein the processing comprises performing spectral unmixing to identify regions of an image associated with one or more signals such as, e.g., fluorescent signals, luminescent signals, and/or colorimetric signals. The invention finds applications in the context of analysis of labelled samples, such as, e.g., for the purpose of detecting and/or quantifying molecules, molecular complexes, cells, subcellular structures, microorganisms, etc.

Abstract: Provided herein are compositions and methods for isolating cell-free nucleic acid, e.g., cell-free DNA, from a sample. In particular embodiments, provided herein are compositions and methods using anti-dsDNA antibodies for isolating cell-free DNA from a sample, and for providing a sample of isolated cell-free DNA, e.g., for a nucleic acid assay. In particular embodiments, the technology relates to providing cell-free DNA from a maternal sample that is enriched for fetal cell-free fetal DNA.

Abstract: Methods of generating cDNAs from RNA involving cleavage of DNA/RNA complexes are provided.

Abstract: Provided herein are compositions and methods for isolating cell-free nucleic acid, e.g., cell-free DNA, from a sample. In particular embodiments, provided herein are compositions and methods using anti-dsDNA antibodies for isolating cell-free DNA from a sample, and for providing a sample of isolated cell-free DNA, e.g., for a nucleic acid assay. In particular embodiments, the technology relates to providing cell-free DNA from a maternal sample that is enriched for fetal cell-free fetal DNA.

Abstract: A method (and system) includes receiving, at a computing device including a design tool application, design parameters indicative of a plurality of power supply loads to be powered. The method further includes generating power supply solutions that do not include multi-channel voltage regulators and generating power supply solutions that do include multi-channel voltage regulators. The method also includes ranking all power supply solutions and providing the ranked power supply solutions to a user.

Abstract: Disclosed herein are methods of vascular and structural imaging using imaging systems and methods described, the methods comprising producing an image of the vasculature or structure by imaging fluorescence using an imaging system, the system comprising: i) one or more detectors configured to form a fluorescence image of the sample and form a visible image of the sample; ii) a light source configured to emit an excitation light to induce fluorescence from the sample; and iii) a plurality of optics arranged to: direct the excitation light toward the sample; and direct a fluorescent light and a visible light from the sample to the detector; wherein the excitation light and the fluorescence light are directed substantially coaxially.

Abstract: Provided herein are compositions and methods for isolating cell-free nucleic acid, e.g., cell-free DNA, from a sample. In particular embodiments, provided herein are compositions and methods using anti-dsDNA antibodies for isolating cell-free DNA from a sample, and for providing a sample of isolated cell-free DNA, e.g., for a nucleic acid assay. In particular embodiments, the technology relates to providing cell-free DNA from a maternal sample that is enriched for fetal cell-free fetal DNA.

Abstract: Disclosed herein are imaging systems and methods for simultaneous near-infrared light and visible light imaging of a sample comprising: a detector to form a fluorescence image of the sample and a visible image of the sample; a light source configured to emit infrared light to induce fluorescence from the sample; and a plurality of optics arranged to direct the infrared light toward the sample and form the fluorescence image of the sample and the visible light image of the sample on the detector, wherein the infrared light is directed to the sample substantially coaxially with fluorescence light received from the sample in order to decrease shadows.

Abstract: A method (and system) includes receiving, at a computing device including a design tool application, design parameters indicative of a plurality of power supply loads to be powered. The method further includes generating power supply solutions that do not include multi-channel voltage regulators and generating power supply solutions that do include multi-channel voltage regulators. The method also includes ranking all power supply solutions and providing the ranked power supply solutions to a user.

Abstract: A method and system includes receiving, at a computing device including a design tool application, design parameters indicative of a plurality of power supply loads to be powered. The method further includes generating power supply solutions that do not include multi-channel voltage regulators and generating power supply solutions that do include multi-channel voltage regulators. The method also includes ranking all power supply solutions and providing the ranked power supply solutions to a user.

Abstract: A method and system includes receiving, at a computing device including a design tool application, design parameters indicative of a plurality of power supply loads to be powered. The method further includes generating power supply solutions that do not include multi-channel voltage regulators and generating power supply solutions that do include multi-channel voltage regulators. The method also includes ranking all power supply solutions and providing the ranked power supply solutions to a user.

Abstract: A method (and system) includes receiving, at a computing device including a design tool application, design parameters indicative of a plurality of power supply loads to be powered. The method further includes generating power supply solutions that do not include multi-channel voltage regulators and generating power supply solutions that do include multi-channel voltage regulators. The method also includes ranking all power supply solutions and providing the ranked power supply solutions to a user.

Abstract: A gas turbine engine includes a turbine section including a plurality of blade outer air seals (BOAS) disposed therein, the BOAS including a BOAS body including a plurality of cooling holes defined in substantial conformance with a set of Cartesian coordinates as set forth in at least one of Table 1 and Table 2.

Abstract: A gas turbine engine includes a turbine section including a plurality of blade outer air seals (BOAS) disposed therein, the BOAS including a BOAS body including a plurality of cooling holes defined in substantial conformance with a set of Cartesian coordinates as set forth in at least one of Table 1 and Table 2.

Abstract: A method of intranasal treatment of nasal symptoms administered to a human in need of the treatment includes administering intranasally from an internasal spray container a composition having a therapeutically effective amount of capsicum extract to a human in need thereof; and effecting relief from at least one symptom. The symptom relieved includes at least one of nasal congestion, sinus pressure, headache, sinus pain or a combination thereof. The first relief of sinus pain, sinus pressure, or nasal decongestion in the human occurs within about 30 minutes of administration.

Abstract: A body fluid sampling device includes a housing, a penetrating member positioned in the housing and a test strip. A compliant front end is coupled to the housing. The compliant front-end includes a rigid member with an interior aperture to receive the body fluid from a wound site.

Abstract: A method (and system) includes receiving, at a computing device including a design tool application, design parameters indicative of a plurality of power supply loads to be powered. The method further includes generating power supply solutions that do not include multi-channel voltage regulators and generating power supply solutions that do include multi-channel voltage regulators. The method also includes ranking all power supply solutions and providing the ranked power supply solutions to a user.

Abstract: A body fluid testing device has at least one penetrating member and a penetrating member driver configured to be coupled to the at least one penetrating member. A plurality of analyte sensors are coupled to a spoked disk substrate. Each of a penetrating member can pass between spokes of the disk substrate to provide that a used penetrating member can be removed and a new penetrating inserted without removing the disk. A disposable houses the at least one penetrating member and the plurality of analyte sensors.