Abstract: The invention describes a two-dimensional system for limiting digital discount offers by time and volume. Digital coupons are entered into a database by way of a web portal, at which time the deal's expiration date and the maximum number of redemptions to be allowed is set. Digital Offers are delivered to consumers' Mobile Devices via a mobile application, which alerts the consumer to the length of the offer period (the expiration date) and the number of available redemptions remaining. Consumers who signal intent to redeem an offer have a specified time period to perform the redemption in-store, otherwise the Digital Offer returns to the available pool. Consumers may exchange rewards “points” in the application for an extension of the time in which they are allowed to redeem the Digital Offer.

Abstract: Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture.

Abstract: Embodiments of labeled nucleotide compositions are described. Methods are described in which a sample containing RNA is contacted with an enzyme having an RNA ligation activity in the presence of a labeled nucleotide composition to provide labeled RNA. Methods of performing an array analysis of a labeled RNA sample are also described.

Abstract: The invention provides generic capture probe arrays, methods of making generic capture probe arrays, and methods of using these arrays to detect target analytes in samples.

Abstract: Oligonucleotide arrays having features that include cleavable oligonucleotides are disclosed, as well as methods of making such arrays. Methods of synthesizing an oligonucleotide on a surface of a substrate are described.

Abstract: Polynucleotide analysis systems and methods of nanopore analysis are provided.

Abstract: In particular embodiments in accordance with the present invention, a wax-embedded tissue specimen is digested and the resulting digested tissue specimen is purified to provide a purified RNA fraction. The purified RNA fraction is treated with one or more enzymes to provide a sample containing dephosphorylated RNA; the sample is contacted with an enzyme having an RNA ligation activity in the presence of a labeled substrate to provide labeled RNA. Kits for performing the described methods are described. Methods of performing an array analysis of a labeled RNA sample are also described.

Abstract: Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture.

Abstract: A method for providing defined mixtures of nucleic acids is described. In certain embodiments, the method uses oligonucleotide probes attached to a solid support as a sequence-specific affinity agent to isolate and facilitate the amplification of defined nucleic acid fragment mixtures.

Abstract: The present invention provides a system for generating nucleic acid molecules having reduced levels of secondary structure compared to nucleic acid molecules of the same nucleotide sequence containing only naturally-occurring bases. Such molecules are referred to herein as “unstructured nucleic acids” (UNAs). UNAs have reduced levels of secondary structure because of their reduced ability to form intramolecular hydrogen bond base pairs between regions of substantially complementary sequence. Preferred UNAs, however, retain the ability to form intermolecular hydrogen bond base pairs with other nucleic acid molecules.

Abstract: The present invention provides a system for generating nucleic acid molecules having reduced levels of secondary structure compared to nucleic acid molecules of the same nucleotide sequence containing only naturally-occurring bases. Such molecules are referred to herein as “unstructured nucleic acids” (UNAs). UNAs have reduced levels of secondary structure because of their reduced ability to form intramolecular hydrogen bond base pairs between regions of substantially complementary sequence. Preferred UNAs, however, retain the ability to form intermolecular hydrogen bond base pairs with other nucleic acid molecules.

Abstract: Articles including a thin laminar heating element composed of a sintered conductive polymer, and electrodes attached to the heating element so that current flows in the plane of the element. The article can be placed between and in contact with two substrates, and then heated to join the two substrates together. Preferably at least one of the substrates is polymeric and becomes melt-fused to the article.