Patents by Inventor Jeffry D. Sander

Jeffry D. Sander has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Using Truncated Guide RNAs (tru-gRNAs) to Increase Specificity for RNA-Guided Genome Editing
    Publication number: 20230407341
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).
    Type: Application
    Filed: March 6, 2023
    Publication date: December 21, 2023
    Inventors: J. Keith Joung, Jeffry D. Sander, Morgan Maeder, Yanfang Fu
  • Using truncated guide RNAs (tru-gRNAs) to increase specificity for RNA-guided genome editing
    Patent number: 11634731
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).
    Type: Grant
    Filed: September 16, 2019
    Date of Patent: April 25, 2023
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Jeffry D. Sander, Morgan Maeder, Yanfang Fu
  • Methods of transcription activator like effector assembly
    Patent number: 11472849
    Abstract: The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and
    Type: Grant
    Filed: February 22, 2019
    Date of Patent: October 18, 2022
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Jeffry D. Sander
  • Increasing Specificity for RNA-Guided Genome Editing
    Publication number: 20210130850
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.
    Type: Application
    Filed: November 16, 2020
    Publication date: May 6, 2021
    Inventors: J. Keith Joung, James Angstman, Jeffry D. Sander, Morgan Maeder, Shengdar Tsai
  • Increasing specificity for RNA-guided genome editing
    Patent number: 10844403
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.
    Type: Grant
    Filed: January 12, 2018
    Date of Patent: November 24, 2020
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, James Angstman, Jeffry D. Sander, Morgan Maeder, Shengdar Tsai
  • Using Truncated Guide RNAs (tru-gRNAs) to Increase Specificity for RNA-Guided Genome Editing
    Publication number: 20200071730
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).
    Type: Application
    Filed: September 16, 2019
    Publication date: March 5, 2020
    Inventors: J. Keith Joung, Jeffry D. Sander, Morgan Maeder, Yanfang Fu
  • Using truncated guide RNAs (tru-gRNAs) to increase specificity for RNA-guided genome editing
    Patent number: 10415059
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).
    Type: Grant
    Filed: February 10, 2017
    Date of Patent: September 17, 2019
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Jeffry D. Sander, Morgan Maeder, Yanfang Fu
  • Methods of Transcription Activator Like Effector Assembly
    Publication number: 20190177374
    Abstract: The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and
    Type: Application
    Filed: February 22, 2019
    Publication date: June 13, 2019
    Inventors: J. Keith Joung, Jeffry D. Sander
  • Methods of transcription activator like effector assembly
    Patent number: 10273271
    Abstract: The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and
    Type: Grant
    Filed: May 17, 2016
    Date of Patent: April 30, 2019
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Jeffry D. Sander
  • Synthetic transcriptional and epigenetic regulators based on engineered, orthogonal zinc finger proteins
    Patent number: 10138493
    Abstract: Embodiments disclosed herein provide artificial expression systems comprising the zinc-finger containing transcription factors and engineered promoters to modulate expression of genes of interest. Engineered zinc-finger transcription factors that interact with engineered promoters constitute synthetic and regulatable expression systems which facilitate the modulation of gene expression as desired.
    Type: Grant
    Filed: August 25, 2017
    Date of Patent: November 27, 2018
    Assignees: TRUSTEES OF BOSTON UNIVERSITY, THE GENERAL HOSPITAL CORPORATION
    Inventors: Ahmad S. Khalil, Divya Israni, Minhee Park, J. Keith Joung, Jeffry D. Sander
  • Using truncated guide RNAs (tru-gRNAs) to increase specificity for RNA-guided genome editing
    Patent number: 10119133
    Abstract: CRISPR-Cas genome editing uses a guide RNA, which includes both a complementarity region, which binds the target DNA by base-pairing, and a Cas9-binding region, to direct a Cas9 nuclease to a target DNA. Further disclosed are methods for increasing specificity of RNA-guided genome editing using CRISPR/Cas9 systems by using truncated guide RNAs (tru-gRNAs).
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: November 6, 2018
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Jeffry D. Sander, Yan-fang Fu, Morgan Maeder
  • Increasing Specificity for RNA-Guided Genome Editing
    Publication number: 20180208921
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.
    Type: Application
    Filed: January 12, 2018
    Publication date: July 26, 2018
    Inventors: J. Keith Joung, James Angstman, Jeffry D. Sander, Morgan Maeder, Shengdar Tsai
  • SYNTHETIC TRANSCRIPTIONAL AND EPIGENETIC REGULATORS BASED ON ENGINEERED, ORTHOGONAL ZINC FINGER PROTEINS
    Publication number: 20180057838
    Abstract: Embodiments disclosed herein provide artificial expression systems comprising the zinc-finger containing transcription factors and engineered promoters to modulate expression of genes of interest. Engineered zinc-finger transcription factors that interact with engineered promoters constitute synthetic and regulatable expression systems which facilitate the modulation of gene expression as desired.
    Type: Application
    Filed: August 25, 2017
    Publication date: March 1, 2018
    Applicants: TRUSTEES OF BOSTON UNIVERSITY, THE GENERAL HOSPITAL CORPORATION
    Inventors: Ahmad S. Khalil, Divya Israni, Minhee Park, J. Keith Joung, Jeffry D. Sander
  • Increasing specificity for RNA-guided genome editing
    Patent number: 9885033
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: February 6, 2018
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, James Angstman, Jeffry D. Sander, Morgan Maeder, Shengdar Tsai
  • Using Truncated Guide RNAs (tru-gRNAs) to Increase Specificity for RNA-Guided Genome Editing
    Publication number: 20170152508
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).
    Type: Application
    Filed: February 10, 2017
    Publication date: June 1, 2017
    Inventors: J. Keith Joung, Jeffry D. Sander, Morgan Maeder, Yanfang Fu
  • Using truncated guide RNAs (tru-gRNAs) to increase specificity for RNA-guided genome editing
    Patent number: 9567604
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: February 14, 2017
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Jeffry D. Sander, Morgan Maeder, Yanfang Fu
  • Methods of Transcription Activator Like Effector Assembly
    Publication number: 20160318978
    Abstract: The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and
    Type: Application
    Filed: May 17, 2016
    Publication date: November 3, 2016
    Inventors: J. Keith Joung, Jeffry D. Sander
  • Increasing Specificity for RNA-Guided Genome Editing
    Publication number: 20160024524
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.
    Type: Application
    Filed: March 14, 2014
    Publication date: January 28, 2016
    Inventors: J. Keith Joung, James Angstman, Jeffry D. Sander, Morgan Maeder, Shengdar Tsai
  • Using Truncated Guide RNAs (tru-gRNAs) to Increase Specificity for RNA-Guided Genome Editing
    Publication number: 20160024523
    Abstract: CRISPR-Cas genome editing uses a guide RNA, which includes both a complementarity region, which binds the target DNA by base-pairing, and a Cas9-binding region, to direct a Cas9 nuclease to a target DNA. Further disclosed are methods for increasing specificity of RNA-guided genome editing using CRISPR/Cas9 systems by using truncated guide RNAs (tru-gRNAs).
    Type: Application
    Filed: March 14, 2014
    Publication date: January 28, 2016
    Inventors: J. Keith Joung, Jeffry D. Sander, Yan-fang Fu, Morgan Maeder
  • Using Truncated Guide RNAs (tru-gRNAs) to Increase Specificity for RNA-Guided Genome Editing
    Publication number: 20140295557
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).
    Type: Application
    Filed: March 14, 2014
    Publication date: October 2, 2014
    Applicant: The General Hospital Corporation
    Inventors: J. Keith Joung, Jeffry D. Sander, Morgan Maeder, Yanfang Fu