Abstract: Provided are a cell identification method for identifying target cells by combining the results of fluorescence intensity analysis, fluorescence image analysis, and white light image analysis.

Abstract: Cell sorting methods are disclosed. A blood sample is provided. The blood sample is centrifuged to obtain a cell mixture having target cells and non-target cells. Fluorescent markers are added to the cell mixture to allow the fluorescent markers to bind with the target cells. A hydrogel solution is added and mixed with the cell mixture to form a mixture solution. The mixture solution is distributed to cell wells of an array chip, and the target cells and non-target cells in the cell mixture are spread on the bottom of the cell wells in a substantially monolayer manner. The mixture solution is cured. A fluorescence image analysis is performed to select the target cells that are bound with fluorescent markers and emit fluorescence, and the selected target cells are isolated.

Abstract: A cell chip includes first, second and third elements, a dye dialysis layer, a micro-channel structure and washing solution inlet and outlet. The first element has a first hole and second holes at opposite sides of the first hole. The second element has a third hole corresponding to the first hole and fourth holes corresponding to the second holes. The dye dialysis layer is inserted between the first element and the second element and has a cell-assembly region corresponding to the first and third holes. The micro-channel structure is disposed below the cell-assembly region and between the second and the third elements. The washing solution inlet and outlet are communicatively connected to the micro-channel structure. The washing solution inlet includes the second hole and a corresponding fourth hole. A washing solution flows in the micro-channel structure through the washing solution inlet and outlet.

Abstract: A sperm sorter and a sperm sorting method are provided. The sperm sorter includes an inlet chamber, a swim up sorting chamber, a divergent channel, a recycling chamber and an outlet chamber. The swim up sorting chamber is communicated with the inlet chamber. The swim up sorting chamber is communicated between the inlet chamber and the divergent channel. The divergent channel has an entrance terminal close to the swim up sorting chamber and an exit terminal away from the swim up sorting chamber. A width and a depth of the entrance terminal are respectively less than a width and a depth of the exit terminal. The recycling chamber is communicated with the exit terminal of the divergent channel. The outlet chamber is communicated with a portion of the divergent channel between the entrance terminal and the exit terminal.

Abstract: A cell chip includes first, second and third elements, a dye dialysis layer, a micro-channel structure and washing solution inlet and outlet. The first element has a first hole and second holes at opposite sides of the first hole. The second element has a third hole corresponding to the first hole and fourth holes corresponding to the second holes. The dye dialysis layer is inserted between the first element and the second element and has a cell-assembly region corresponding to the first and third holes. The micro-channel structure is disposed below the cell-assembly region and between the second and the third elements. The washing solution inlet and outlet are communicatively connected to the micro-channel structure. The washing solution inlet includes the second hole and a corresponding fourth hole. A washing solution flows in the micro-channel structure through the washing solution inlet and outlet.

Abstract: A metal ion detection equipment and a metal ion detection method are provided. The metal ion detection equipment includes a porous silicon resonant cavity structure, an electrochemical device and a spectrum detecting device. A sample solution permeates into the porous silicon resonant cavity structure. A to-be-detected metal ion of the sample solution in the porous silicon resonant cavity structure is reduced into a to-be-detected metal by the electrochemical device. The spectrum detecting device detects a spectral variation of a reflective light from the porous silicon resonant cavity structure.

Abstract: This invention relates to microfluidic chips for and their applications in acquiring sperms with high velocity and/or motility. The microfluidic chip comprises an inlet region, a first flow channel, a divergent channel, an optional block structure with rounded corners and one or more outlet region(s). The invention mimics sperm activation process in body and designs a microfluidic chip mimicking the activation process so that higher amount of populations and/or subpopulations of sperms with high motility can be acquired.

Abstract: A metal ion detection equipment and a metal ion detection method are provided. The metal ion detection equipment includes a porous silicon resonant cavity structure, an electrochemical device and a spectrum detecting device. A sample solution permeates into the porous silicon resonant cavity structure. A to-be-detected metal ion of the sample solution in the porous silicon resonant cavity structure is reduced into a to-be-detected metal by the electrochemical device. The spectrum detecting device detects a spectral variation of a reflective light from the porous silicon resonant cavity structure.

Abstract: The present invention is to provide a method of performing electropolymerized electrochemically active poly-films as a current signal to detect bacteremia. The method comprises conjugating an electrochemical redox-active molecular monomer and a specific antibody with a gold nanoparticle to form a modified gold nanoparticle, and the modified gold nanoparticles are conjugated to the surface of bacteria via a specific antibody to form an electrochemically active poly-film by electropolymerization. When applying a voltage, a redox-active current signal of the electropolymerized electrochemically active poly-films can be detected by a usual electrochemical detection system typically in the range between nA and mA.

Abstract: This disclosure provides a desalination apparatus having a first electrode plate, a first filtering unit, a second filtering unit and a second electrode plate arranged in sequence as well as a power supply unit. Each of the filtering units comprising: an insulation substrate having a plurality of trench holes penetrating through the insulation substrate; a conductive layer formed on the insulation substrate and sidewalls of the plurality of trench holes; and an insulation layer formed on the conductive layer and the sidewalls of the plurality of trench holes; wherein the power supply unit provides the first filtering unit, the second filtering unit, the first electrode plate and the second electrode plate with a first electric potential of positive value, a second electric potential of negative value, a third electric potential and a fourth electric potential, respectively, and the third electric potential is larger than the fourth electric potential.

Abstract: The present invention discloses a sperm selecting system and the method thereof. The brief concept of the present invention is to generate a flow field by hydraulic pressure difference then utilize the property that the sperm swims against the flow field so as to difference the sperms by vitality thereof. One of the main features of the present invention is that the sperms be selected are initially set at the entrance of the flow field instead of the exit of the system. Furthermore, an activating design can be selectively added to the present invention so as to activate the sperm be affected by the process of freeze storing.

Abstract: The present invention discloses a sperm selecting system and the method thereof. The brief concept of the present invention is to generate a flow field by hydraulic pressure difference then utilize the property that the sperm swims against the flow field so as to difference the sperms by vitality thereof. One of the main features of the present invention is that the sperms be selected are initially set at the entrance of the flow field instead of the exit of the system. Furthermore, an activating design can be selectively added to the present invention so as to activate the sperm be affected by the process of freeze storing.

Abstract: A method of yield management for semiconductor manufacture and an apparatus thereof are provided. The method includes the following steps. Defect data of a layer of a semiconductor wafer is obtained, wherein the defect data includes sizes and locations of defects with respect to the layer. A layout with respect to the layer is obtained. And a critical area analysis is performed in parallel for the layer by a plurality of processing devices according to the defect data and the layout to determine locations of defects falling into a critical area of the layer among the locations of the defects.

Abstract: A method of yield management for semiconductor manufacture and an apparatus thereof are provided. The method includes the following steps. Defect data of a layer of a semiconductor wafer is obtained, wherein the defect data includes sizes and locations of defects with respect to the layer. A layout with respect to the layer is obtained. And a critical area analysis is performed in parallel for the layer by a plurality of processing devices according to the defect data and the layout to determine locations of defects falling into a critical area of the layer among the locations of the defects.