Patents by Inventor Jen-Leih Wu
Jen-Leih Wu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20140066379Abstract: A recombinant vector, transgenic fish egg using the same and biomaterial using the same are applied to provide a transgenic fish that secreting recombinant human procollagens or collagens, and further to provide the biomaterial having the recombinant human procollagens or collagens and extract the recombinant human procollagens or collagens from the part(s), having the recombinant human procollagens or collagens, of the transgenic fish.Type: ApplicationFiled: August 28, 2013Publication date: March 6, 2014Applicant: Body Organ Biomedical Corp.Inventors: Chuian-Fu Ken, Hong-Yi Gong, Jen-Leih Wu, Horng-Ji Lai, Chien-Cheng Lin, Yong-Guei Chen, Cheng-Hung Chou
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Patent number: 8383880Abstract: A method of inducing male and/or female infertility in a genetically modified (GM) fish is disclosed. Also disclosed is a method of generating an infertile GM fish with a phenotype and/or genotype of interest. The method involves generation of a GM fish whose genome comprises a foreign transgene operably linked to a fish gonad-specific promoter selected from the group consisting of an ovary-specific promoter and a testis-specific promoter. The transgene comprises a suicide gene selected from the group consisting of a reductase and a photosensitizer, in which the reductase is operably linked to a reporter gene. Infertility of the GM fish is induced if the GM fish expressing the reductase is treated with an effective amount of a reductase-activated cytotoxic prodrug or if the GM fish expressing the photosensitizer is treated with light irradiation.Type: GrantFiled: February 12, 2010Date of Patent: February 26, 2013Assignees: Academia Sinica, National Taiwan Ocean UniversityInventors: Jen-Leih Wu, Shao-Yang Hu, Guor Mour Her
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Publication number: 20100212039Abstract: A method of inducing male and/or female infertility in a genetically modified (GM) fish is disclosed. Also disclosed is a method of generating an infertile GM fish with a phenotype and/or genotype of interest. The method involves generation of a GM fish whose genome comprises a foreign transgene operably linked to a fish gonad-specific promoter selected from the group consisting of an ovary-specific promoter and a testis-specific promoter. The transgene comprises a suicide gene selected from the group consisting of a reductase and a photosensitizer, in which the reductase is operably linked to a reporter gene. Infertility of the GM fish is induced if the GM fish expressing the reductase is treated with an effective amount of a reductase-activated cytotoxic prodrug or if the GM fish expressing the photosensitizer is treated with light irradiation.Type: ApplicationFiled: February 12, 2010Publication date: August 19, 2010Applicant: ACADEMIA SINICAInventors: JEN-LEIH WU, SHAO-YANG HU, GUOR MOUR HER
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Patent number: 7670836Abstract: The present invention provides an antimicrobial peptide, monodoncin, which is isolated and purified from Penaeus monodon and is capable of being mass produced by molecular cloning techniques in a heterologous expression system, such as yeast. Monodoncin demonstrates a wide-range of bacteriostatic and bactericidal effects on G(?) and G(+) bacteria as well as fungicidal activities, and can be used in combination with conventional antibiotics as “cocktail therapy” to improve the therapeutic effects of the conventional antibiotics.Type: GrantFiled: May 30, 2007Date of Patent: March 2, 2010Assignee: Academia SinicaInventors: Jenn-Kan Lu, Jen-Leih Wu, Tze-Ting Chiou
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Patent number: 7411060Abstract: The present invention relates to expression control sequences of a vertebrate liver fatty acid binding protein (L-FABP) gene that, when operably linked to a reporter (e.g., a heterologous reporter, such as the green fluorescent protein (GFP)), directly express the reporter in a fashion that mimics the liver-specific development of the L-FABP gene in the vertebrate.Type: GrantFiled: November 21, 2003Date of Patent: August 12, 2008Assignee: Academia SinicaInventors: Jen-Leih Wu, Guor Mour Her
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Publication number: 20080032385Abstract: The present invention provides an antimicrobial peptide, monodoncin, which is isolated and purified from Penaeus monodon and is capable of being mass produced by molecular cloning techniques in a heterologous expression system, such as yeast. Monodoncin demonstrates a wide-range of bacteriostatic and bactericidal effects on G (?) and G (+) bacteria as well as fungicidal activities, and can be used in combination with conventional antibiotics as “cocktail therapy” to improve the therapeutic effects of the conventional antibiotics.Type: ApplicationFiled: May 30, 2007Publication date: February 7, 2008Inventors: Jenn-Kan Lu, Jen-Leih Wu, Tze-Ting Chiou
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Patent number: 7008621Abstract: The present invention provides a mechanism for studies of apoptosis in aquatic organisms by infecting the aquatic organisms with aquabirnavirus, especially infectious pancreatic necrosis virus (IPNV). The infection of IPNV in an aquatic cell such as a Chinook salmon embryo cell (CHSE-214) converts the cell into an apoptotic cell. The present invention also provides a method for monitoring the morphological changes during apoptosis by cloning EGFP (a variant type of GFP) to an aquatic cell and monitoring the fluorescence using microscopic techniques. The intensity of the fluorescence reflects the expression of EGFP in cells. Finally, the present invention provides means for inducing or preventing/rescuing apoptosis in a host, which include aquatic and vertebrate. The apoptosis can be induced by IPNV infection or VP3 transfection that VP3 is a 32 kDa protein derived from IPNV segment A. The apoptosis can be prevented or rescued by an antisense VP3 RNA or a zfMcl-1a protein.Type: GrantFiled: March 21, 2003Date of Patent: March 7, 2006Assignee: Academia SinicaInventors: Jen-Leih Wu, Jiann-Ruey Hong
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Publication number: 20040235738Abstract: The present invention provides an antimicrobial peptide, monodoncin, which is isolated and purified from Penaeus monodon and is capable of being mass produced by molecular cloning techniques in a heterologous expression system, such as yeast. Monodoncin demonstrates a wide-range of bacteriostatic and bactericidal effects on G(−) and G(+) bacteria as well as fungicidal activities, and can be used in combination with conventional antibiotics as “cocktail therapy” to improve the therapeutic effects of the conventional antibiotics.Type: ApplicationFiled: May 10, 2004Publication date: November 25, 2004Applicant: Academia SinicaInventors: Jenn-Kan Lu, Jen-Leih Wu, Tze-Ting Chiou
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Publication number: 20040209279Abstract: The present invention relates to expression control sequences of a vertebrate liver fatty acid binding protein (L-FABP) gene that, when operably linked to a reporter (e.g., a heterologous reporter, such as the green fluorescent protein (GFP)), directly express the reporter in a fashion that mimics the liver-specific development of the L-FABP gene in the vertebrate. Also disclosed is transgenic fish, such as a transgenic zebrafish, whose cells comprises at least one genomically integrated copy of a recombinant construct comprising such an expression control sequence, operably linked to a reporter sequence, so that the expression of the reporter is liver-cell specific, both spatially and temporally during development.Type: ApplicationFiled: October 3, 2003Publication date: October 21, 2004Inventors: Jen-Leih Wu, Guor Mour Her
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Publication number: 20040209833Abstract: The present invention relates to expression control sequences of a vertebrate liver fatty acid binding protein (L-FABP) gene that, when operably linked to a reporter (e.g., a heterologous reporter, such as the green fluorescent protein (GFP)), directly express the reporter in a fashion that mimics the liver-specific development of the L-FABP gene in the vertebrate. Also disclosed is transgenic fish, such as a transgenic zebrafish, whose cells comprises at least one genomically integrated copy of a recombinant construct comprising such an expression control sequence, operably linked to a reporter sequence, so that the expression of the reporter is liver-cell specific, both spatially and temporally during development.Type: ApplicationFiled: November 21, 2003Publication date: October 21, 2004Inventors: Jen-Leih Wu, Guor Mour Her
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Patent number: 6630456Abstract: The present invention provides a mechanism for studies of apoptosis in aquatic organisms by infecting the aquatic organisms with aquabirnavirus, especially infectious pancreatic necrosis virus (IPNV). The infection of IPNV in an aquatic cell such as a Chinook salmon embryo cell (CHSE-214) converts the cell into an apoptotic cell. The present invention also provides a method for monitoring the morphological changes during apoptosis by cloning EGFP (a variant type of GFP) to an aquatic cell and monitoring the fluorescence using microscopic techniques. The intensity of the fluorescence reflects the expression of EGEP in cells. Finally, the present invention provides means for inducing or preventing/rescuing apoptosis in a host, which include aquatic and vertebrate. The apoptosis can be induced by IPNV infection or VP3 transfection.that VP3 is a 32 kDa protein derived from IPNV segment A. The apoptosis can be prevented or rescued by an antisense VP3 RNA or a zfMcl-1a protein.Type: GrantFiled: February 25, 2002Date of Patent: October 7, 2003Assignee: Academia SinicaInventors: Jen-Leih Wu, Jiann-Ruey Hong
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Publication number: 20030180325Abstract: The present invention provides a mechanism for studies of apoptosis in aquatic organisms by infecting the aquatic organisms with aquabirnavirus, especially infectious pancreatic necrosis virus (IPNV). The infection of IPNV in an aquatic cell such as a Chinook salmon embryo cell (CHSE-214) converts the cell into an apoptotic cell. The present invention also provides a method for monitoring the morphological changes during apoptosis by cloning EGFP (a variant type of GFP) to an aquatic cell and monitoring the fluorescence using microscopic techniques. The intensity of the fluorescence reflects the expression of EGFP in cells. Finally, the present invention provides means for inducing or preventing/rescuing apoptosis in a host, which include aquatic and vertebrate. The apoptosis can be induced by IPNV infection or VP3 transfection. that VP3 is a 32 kDa protein derived from IPNV segment A. The apoptosis can be prevented or rescued by an antisense VP3 RNA or a zfMc1-1a protein.Type: ApplicationFiled: March 21, 2003Publication date: September 25, 2003Inventors: Jen-Leih Wu, Jiann-Ruey Hong
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Publication number: 20020103128Abstract: The present invention provides a mechanism for studies of apoptosis in aquatic organisms by infecting the aquatic organisms with aquabimavirus, especially infectious pancreatic necrosis virus (IPNV). The infection of IPNV in an aquatic cell such as a Chinook salmon embryo cell (CHSE-214) converts the cell into an apoptotic cell. The present invention also provides a method for monitoring the morphological changes during apoptosis by cloning EGFP (a variant type of GFP) to an aquatic cell and monitoring the fluorescence using microscopic techniques. The intensity of the fluorescence reflects the expression of EGFP in cells. Finally, the present invention provides means for inducing or preventing/rescuing apoptosis in a host, which include aquatic and vertebrate. The apoptosis can be induced by IPNV infection or VP3 transfection.that VP3 is a 32 kDa protein derived from IPNV segment A. The apoptosis can be prevented or rescued by an antisense VP3 RNA or a zfMcl-1a protein.Type: ApplicationFiled: February 25, 2002Publication date: August 1, 2002Inventors: Jen-Leih Wu, Jiann-Ruey Hong
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Patent number: 6235874Abstract: The present invention relates to the finding and construction of fish insulin-like growth factor II (IGF-II) cDNAs which can be cloned and expressed in cells. This invention also relates to the production of biologically active fish IGF-II polypeptides by a gene expression system using fish IGF-II cDNAs. The fish IGF-II cDNAs have 1977 bp which transcribe into a prepeptide (signal peptide), and B, C, A, D, E domain peptides. The fish mature IGF-II is a single polypeptide containing the NH2—B—C—A—D—COOH domains. The mature IGF-II polypeptide is 7 kDa in weight and has 70 amino acids. The fish recombinant IGF-II cDNA can be cloned and expressed in E. coli, yeast, baculovirus, and fish cells. The isolated and purified IGF-II E domain peptide has mitogenic and anti-tumor activity.Type: GrantFiled: August 26, 1999Date of Patent: May 22, 2001Assignee: Academia SinicaInventors: Jen-Leih Wu, Jyh-Yih Chen
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Patent number: 6207817Abstract: This present invention relates to the findings of the DNA sequences of fish insulin-like growth factor II (IGF-II) promoter regions and recombinant IGF-II promoters. These DNA sequences are capable of being expressed in eukaryotic cells and fish embryos of another fish species. The integration of the IGF-II promoter regions or recombinant IGF-II promoters into fish of another species results in the creation of a transgenic fish. The results of this invention illustrate that a fish IGF-II promoter not only can act as a growth factor to stimulate the growth and development of fish, but also is capable of being expressed in other eukaryotic cells such as in human cells.Type: GrantFiled: October 7, 1999Date of Patent: March 27, 2001Assignee: Academia SinicaInventors: Jen-Leih Wu, Jyh-Yih Chen
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Patent number: 6010882Abstract: The present invention relates to the finding and construction of fish insulin-like growth factor II (IGF-II) cDNAs which can be cloned and expressed in cells. This invention also relates to the production of biologically active fish IGF-II polypeptides by a gene expression system using fish IGF-II cDNAs. The fish IGF-II cDNAs have 1977 bp which transcribe into a prepeptide (signal peptide), and B, C, A, D, E domain peptides. The fish mature IGF-II is a single polypeptide containing the NH.sub.2 -B-C-A-D-COOH domains. The mature IGF-II polypeptide is 7 kDa in weight and has 70 amino acids. The fish recombinant IGF-II CDNA can be cloned and expressed in E. coli, yeast, baculovirus, and fish cells.Type: GrantFiled: January 7, 1998Date of Patent: January 4, 2000Assignee: Academia SinicaInventors: Jen-Leih Wu, Jyh-Yih Chen, Chi-Yao Chang