Abstract: A tissue culture method for producing agarwood comprises a tissue culture/regeneration step: providing an agarwood explant, implanting the agarwood explant in a container under an antiseptic state, adding culture media into the container, and cultivating the agarwood explant into an agarwood plantlet; a microbe inoculation step: placing a resin-inducing agent into the container, and letting the resin-inducing agent invade the agarwood plantlet; and a resin formation step: letting the agarwood plantlet secrete a secondary metabolite to counteract the resin-inducing agent, and letting the secondary metabolite deposit to form agarwood. The present invention can mass-produce agarwood in an indoor environment without being limited by weather or soil quality and affected by variables (such as insect pests) existing in the natural environment, The present invention can further shorten the production period and accelerate cost recovery. Therefore, the present invention can satisfy requirements of manufacturers.