Abstract: Surfactin is produced from Bacillus subtilis ssp. containing sfp gene (lipopeptide biosurfactants produced by fermentation). Production of surfactin at present is mainly by liquid fermentation, but the production costs are high due to difficulty in purification resulted from addition of the defoaming agent during the production process. Therefore, present invention conducts physical or chemical mutation on Bacillus subtilis subsp. isolated from Thailand seawater shrimp ponds and screens for the mutant strain of Bacillus subtilis subsp. based on sfp gene expression and then produces surfactin from the mutant strain by semi-solid state fermentation.

Abstract: Surfactin is produced from Bacillus subtilis ssp. containing sfp gene (lipopeptide biosurfactants produced by fermentation). Production of surfactin at present is mainly by liquid fermentation, but the production costs are high due to difficulty in purification resulted from addition of the defoaming agent during the production process. Therefore, present invention conducts physical or chemical mutation on Bacillus subtilis subsp. isolated from Thailand seawater shrimp ponds and screens for the mutant strain of Bacillus subtilis subsp. based on sfp gene expression and then produces surfactin from the mutant strain by semi-solid state fermentation.

Abstract: A method for preparation of a feed additive containing surfactin, which uses a high-yield mutant strain, Bacillus subtilis T, and soybeans as the substrate in semi-solid state fermentation and after drying and grinding of the fermented soybeans into soybean powder to give a feed additive containing surfactin. Each kilogram of the fermented soybean powder contains about 6-7 g of surfactin. Feeding grouper with the feed additive containing surfactin can promote growth rate, increase expression of immune genes (e.g. AMP, Mx and IFN-induced protein), and lower mortality rate of virus-infected fish, indicating the feed containing surfactin has the effects of growth promotion, immunity enhancement and provides resistance to pathogen infection.

Abstract: Surfactin is a biosurfactant produced by Bacillus subtilis and is a natural cycloaliphatic peptide having a ring structure made of 7 amino acids. The surfactin has various functions including anti-aging, anti-wrinkle, increasing skin penetration of cosmetic products (skin penetration agent), foaming agent, and emulsifier.

Abstract: Surfactin is a biosurfactant produced by Bacillus subtilis and said surfactin is a natural cycloaliphatic peptide comprising a ring structure made of 7 amino acids, and said surfactin has various functions including anti-aging, anti-wrinkle, increasing skin penetration of cosmetic products (skin penetration agent), foaming agent and emulsifier.

Abstract: Surfactin is a biosurfactant produced by Bacillus subtilis and is a natural cycloaliphatic peptide having a ring structure made of 7 amino acids. The surfactin has various functions including anti-aging, anti-wrinkle, increasing skin penetration of cosmetic products (skin penetration agent), foaming agent, and emulsifier.

Abstract: An intelligent canopy greenhouse control system is provided with two main brackets symmetrically arranged, each of which penetrates a curved tubular beam at its upper edge for the curved tubular beam's one end to protrude from each main bracket. Several small crossbeams are installed between two curved tubular beams for a reinforced structure. At least a louvered shutter is provided between two curved tubular beams and between two main brackets. A film is covered between two curved tubular beams, and steel sheets are freely connected to gaps between two main brackets and under the lower edges of two curved tubular beams for development of a first skeleton unit with gutters installed, around which a canopy greenhouse can be assembled with a plurality of first skeleton units.

Abstract: An intelligent canopy greenhouse control system is provided with two main brackets symmetrically arranged, each of which penetrates a curved tubular beam at its upper edge for the curved tubular beam's one end to protrude from each main bracket. Several small crossbeams are installed between two curved tubular beams for a reinforced structure. At least a louvered shutter is provided between two curved tubular beams and between two main brackets. A film is covered between two curved tubular beams, and steel sheets are freely connected to gaps between two main brackets and under the lower edges of two curved tubular beams for development of a first skeleton unit with gutters installed, around which a canopy greenhouse can be assembled with a plurality of first skeleton units.

Abstract: The present invention provides an antimicrobial peptide, monodoncin, which is isolated and purified from Penaeus monodon and is capable of being mass produced by molecular cloning techniques in a heterologous expression system, such as yeast. Monodoncin demonstrates a wide-range of bacteriostatic and bactericidal effects on G(?) and G(+) bacteria as well as fungicidal activities, and can be used in combination with conventional antibiotics as “cocktail therapy” to improve the therapeutic effects of the conventional antibiotics.

Abstract: The present invention provides an antimicrobial peptide, monodoncin, which is isolated and purified from Penaeus monodon and is capable of being mass produced by molecular cloning techniques in a heterologous expression system, such as yeast. Monodoncin demonstrates a wide-range of bacteriostatic and bactericidal effects on G (?) and G (+) bacteria as well as fungicidal activities, and can be used in combination with conventional antibiotics as “cocktail therapy” to improve the therapeutic effects of the conventional antibiotics.

Abstract: The present invention provides an antimicrobial peptide, monodoncin, which is isolated and purified from Penaeus monodon and is capable of being mass produced by molecular cloning techniques in a heterologous expression system, such as yeast. Monodoncin demonstrates a wide-range of bacteriostatic and bactericidal effects on G(−) and G(+) bacteria as well as fungicidal activities, and can be used in combination with conventional antibiotics as “cocktail therapy” to improve the therapeutic effects of the conventional antibiotics.