Abstract: Described are methods of enhancing development of renal organoids, methods of using the same, and kits.

Abstract: Described are methods for producing multi-layered tubular tissue structures, tissue structures produced by the methods, and their use.

Abstract: A method of printing a cellular solid by direct bubble writing comprises introducing an ink formulation comprising a polymerizable monomer and a gas into a nozzle, which includes a core flow channel radially surrounded by an outer flow channel. The ink formulation is directed into the outer flow channel and the gas is directed into the core flow channel. The ink formulation and the gas are ejected out of the nozzle as a stream of bubbles, where each bubble includes a core comprising the gas and a liquid shell overlying the core that comprises the ink formulation. After ejection, the polymerizable monomer is polymerized to form a solid polymeric shell from the liquid shell, and the bubbles are deposited on a substrate moving relative to the nozzle. Thus, a polymeric cellular solid having a predetermined geometry is printed.

Abstract: A composition including a particle including a crosslinked matrix, prepared from a liquid droplet including a formulation including a polymer, the gelled particle having an average diameter of less than 250 pm, is provided herein. A method of printing the composition including releasing the liquid droplet into a fluid including an aerosol including a crosslinking agent and contacting the liquid droplet with the fluid is further provided. A device configured to introduce an aerosol including a crosslinking agent to a droplet of a formulation including a polymer as the droplet passes through the aerosol including the crosslinking agent is further provided.

Abstract: A method of acoustophoretic printing comprises generating an acoustic field at a first end of an acoustic chamber fully or partially enclosed by sound-reflecting walls. The acoustic field interacts with the sound-reflecting walls and travels through the acoustic chamber. The acoustic field is enhanced in a chamber outlet at a second end of the acoustic chamber. An ink is delivered into a nozzle positioned within the acoustic chamber. The nozzle has a nozzle opening projecting into the chamber outlet. The ink travels through the nozzle and is exposed to the enhanced acoustic field at the nozzle opening, and a predetermined volume of the ink is ejected from the nozzle opening and out of the acoustic chamber.

Abstract: Described herein are two-layer 3-D cultures, including those with microfeatures at the boundary between the two layers, as well as methods of making and using such cultures.

Abstract: A printed composition for biomedical uses comprises a liquid droplet prior to crosslinking and a gelled particle after crosslinking, where the liquid droplet comprises a formulation including a hydrogel precursor and abiologic, and the gelled particle comprises a cross-linked hydrogel matrix with the biologic dispersed therein. The formulation has a viscosity in a range from about 100 mPa-s to about 500,000 mPa-s.

Abstract: A bioink for extrusion-based printing includes an extracellular matrix (ECM) precursor comprising an uncrosslinked polymer, and sacrificial microparticles dispersed in the ECM precursor. The sacrificial microparticles have a melting temperature above a crosslinking temperature of the uncrosslinked polymer. A method of fabricating a tissue/organ model or therapeutic construct comprises extruding a bioink comprising a first ECM precursor and first sacrificial microparticles through a nozzle moving relative to a deposition bath, and depositing an extruded filament comprising the bioink into the deposition bath as the nozzle moves. After deposition, the first ECM precursor is crosslinked to form a first ECM material, and after the crosslinking, the first sacrificial microparticles are melted to form pores in the first ECM material. The pores may have a width or diameter comparable to that of individual cells.

Abstract: A method of acoustophoretic printing comprises generating an acoustic field at a first end of an acoustic chamber fully or partially enclosed by sound-reflecting walls. The acoustic field interacts with the sound-reflecting walls and travels through the acoustic chamber. The acoustic field is enhanced in a chamber outlet at a second end of the acoustic chamber. An ink is delivered into a nozzle positioned within the acoustic chamber. The nozzle has a nozzle opening projecting into the chamber outlet. The ink travels through the nozzle and is exposed to the enhanced acoustic field at the nozzle opening, and a predetermined volume of the ink is ejected from the nozzle opening and out of the acoustic chamber.

Abstract: A filamentary structure extruded from a nozzle during 3D printing comprises a continuous filament including filler particles dispersed therein. At least some fraction of the filler particles in the continuous filament comprise high aspect ratio particles having a predetermined orientation with respect to a longitudinal axis of the continuous filament. The high aspect ratio particles may be at least partially aligned along the longitudinal axis of the continuous filament. In some embodiments, the high aspect ratio particles may be highly aligned along the longitudinal axis. Also or alternatively, at least some fraction of the high aspect ratio particles may have a helical orientation comprising a circumferential component and a longitudinal component, where the circumferential component is imparted by rotation of a deposition nozzle and the longitudinal component is imparted by translation of the deposition nozzle.

Abstract: A printhead comprises a plurality of ink cartridges and a nozzle, where the nozzle and the ink cartridges are configured to rotate together about an axis during printing. The nozzle includes a nozzle body comprising an inlet end, an outlet end, and one or more internal passageways extending through the nozzle body from the inlet end to the outlet end. The one or more internal passageways terminate at one or more outlets at or near the outlet end. The nozzle also includes plurality of nozzle inlets at the inlet end for delivery of flowable inks into the internal passageways, where each nozzle inlet is in fluid communication with a dispensing end of one of the ink cartridges.

Abstract: A product includes a three-dimensional structure having a plurality of sequentially-formed layers comprised of liquid crystal elastomers. The liquid crystal elastomers in at least a predefined portion of a first of the layers are substantially aligned in a first orientation, and the liquid crystal elastomers in at least a predefined portion of a second of the layers are substantially aligned in a second orientation. In addition, each of the portions of the three-dimensional structure is characterized as exhibiting a shape change in response to a stimulus, where the shape change is reversible.

Abstract: Viscoelastic hydrogel microparticles are used for repair of tissue defects and injuries or filling and occlusion of anatomical structures. These are administered as a microparticle suspension using a catheter, syringe, steerable catheter tip, or comparable technology into the site, where they can be further stabilized by crosslinking or sealing, or through incorporation of a support or encapsulating structure. Materials and methods for solidifying, stabilizing and sealing these materials can be used that are also biocompatible and easily deployed with catheters in the body. The micron sized interstitial spacing provides a scaffold for ingrowth and migration of cells into the gel matrices.

Abstract: In one aspect, the present disclosure provides a nozzle for a 3D printing system. The nozzle may include a flowpath with a material inlet and a material outlet. The nozzle may further include a valve in fluid communication with the flowpath between the material inlet and the material outlet, where the valve includes a closed state and an open state, where in the closed state the valve obstructs the flowpath between the material inlet and the material outlet, and where in the open state the material inlet is in fluid communication with the material outlet. The nozzle may further include a compensator in fluid communication with the flowpath, where the compensator includes a contracted state associated with the open state of the valve and an expanded state associated with the closed state of the valve.

Abstract: In one aspect, the present disclosure provides a nozzle for a 3D printing system. The nozzle may include a flowpath with a material inlet and a material outlet. The nozzle may further include a valve in fluid communication with the flowpath between the material inlet and the material outlet, where the valve includes a closed state and an open state, where in the closed state the valve obstructs the flowpath between the material inlet and the material outlet, and where in the open state the material inlet is in fluid communication with the material outlet. The nozzle may further include a compensator in fluid communication with the flowpath, where the compensator includes a contracted state associated with the open state of the valve and an expanded state associated with the closed state of the valve.

Abstract: A subwavelength resonator for acoustophoretic printing comprises a hollow resonator body for local enhancement of an acoustic field integrated with a nozzle body for delivery of an ink into the acoustic field. The nozzle body has a first end outside the hollow resonator body and a second end inside the hollow resonator body, and includes a fluid channel extending between a fluid inlet at the first end and a fluid outlet at the second end. The fluid channel passes through a side wall of the hollow resonator body and includes at least one bend. During acoustophoretic printing, an ink delivered through the fluid channel of the nozzle body and out of the fluid outlet is exposed to a high-intensity acoustic field.

Abstract: A 3-D printed device comprising one or more structures, the structures comprising a plurality of magnetically responsive particles and one or more diblock or triblock copolymers; the diblock or triblock copolymers having an A-B, A-B-A, or A-B-C block-type structure in which the A-blocks and C-blocks are an aromatic-based polymer or an acrylate-based polymer and the B-blocks are an aliphatic-based polymer. These 3-D printed devices may be formed using a method that comprises providing a magnetic ink composition; applying the magnetic ink composition to a substrate in a 3-D solvent cast printing process to form one or more structures; and drying the one or more structures formed from the magnetic ink composition. The dried structures can exhibit one or more regions of magnetic permeability greater than 1.3×10?6 H/m.

Abstract: A method of controlling macroscopic properties of a metamaterial includes 3D printing a lattice structure comprising interconnected struts, where each strut comprises one or more printed filaments. Each printed filament comprises an active material or a passive material, and the active material has a modulus with a higher stimulus dependence than that of the passive material. The printed filaments comprising the active material are disposed at predetermined regions of the lattice structure. After 3D printing, the lattice structure is exposed to a stimulus, and the predetermined regions comprising the active material soften or stiffen. Thus, the macroscopic properties of the lattice structure may be controlled.

Abstract: A modular roller hemming system having a base assembly, a replaceable anvil, a spider arm assembly, a plurality of support arms for supporting the spider arm assembly, and a plurality of repositionable unit tools. The anvil is 3-D printed of a polymer composite material and may be replaced with similarly manufactured anvils having different form factors for receiving various shaped and dimensioned workpiece assemblies. The plurality of support arms are repositionable on the base assembly, the spider arm assembly is reconfigurable, and the plurality of unit tools are moveable to accommodate various anvils having different form factors. The support arms includes an upper segment that is detachable from the lower segment to facilitate the changeover of anvils.

Abstract: Described herein are methods of generating a programmable multicellular organoid and/or a 3D organ-specific tissue. Also, described are the programmable multicellular organoid and/or a 3D organ-specific tissue produced by the described methods. Also, described herein are in vitro methods of generating functional human tissue construct.