Abstract: Disclosed are methods for amplifying and sequencing target segments of nucleotides. In the method, nucleic acids are amplified using at least two oligonucleotide primers, wherein at least one of the primers contains a complimentary region that hybridizes adjacent to the target segment of nucleotides and an extender region that includes a primer hybridization sequence. Also included are methods of amplification with an extender region includes a sequence encoding a promoter for an RNA polymerase and optionally sequences for translation. The latter amplified products may be used for transcription of a RNA including subsequent translation of the RNA to generate a protein product.

Abstract: The present invention provides methods of determining the genotype at a duplicated region of a genome. The methods involve (a) amplifying the DNA, preferably using four amplification primers The exemplary methods produce a first amplicon corresponding to a first distinct region or gene by utilizing a first primer and a second primer which are selected to produce the first amplicon in the presence of the first distinct region or gene, but not in absence of the first distinct region or gene. A second amplicon corresponding to a second distinct region or gene is also produced by utilizing a third primer and a fourth primer which are selected to produce the second amplicon in the presence of the second distinct region or gene, but not in the absence of the second distinct region or gene. A third and/or fourth amplicon corresponding to a hybrid gene or genes is/are also produced if a hybrid gene is present.