Abstract: A system and method for detecting trace explosives is provided that utilizes a lumnescent reagent, such as blue-fluorescent biphenyl compound, to simultaneously detect multiple nitrogen-based explosives on a sample substrate. The test reagent is mixed with a solvent to improve mixing and maximize the dissolution of the reagent and any trace explosives present on the substrate. A thin film of reagent is applied to the substrate either before or after the substrate contacts a sample area to be tested. Heated air is then applied to the substrate to improve the sample reading. A light source is utilized to illuminate the reagent and expose any quenched portions of the substrate that indicate the presence of an explosive. Quenched portions may be detected visually, or utilizing an analyzing apparatus, such as a fluorimeter or camera. A computer may also be utilized to interpret and record test results.