Abstract: The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.

Abstract: A novel prefabricated garden box for use in growing plants with a raised bed, comprising a plurality of posts, beams, crossbeams, an inclined roof and slidably rigid transparent or translucent door(s). The enclosure includes multi-channel slidetracks and is fillable with soil and gravel under conditions that provide a suitable growing environment. The garden box, when fully assembled, rests on a ground surface or subgrade and may be preferably constructed to conform to dimensions suitable for shipping, packaging and transport before assembly by an end user/gardener.

Abstract: A versatile dust ruffle includes means for vertically adjusting the position of the dust ruffle on any box spring and bed frame combination. Further, the dust ruffle includes means for adjusting the dust ruffle to fit the perimeter of any size mattress. One or more resilient strips affixed to the dust ruffle and one or more adjustable straps joining ends of the dust ruffle facilitate the unique attributes of the dust ruffle. Importantly, the placement of the dust ruffle is accomplished without having to move or adjust a subject mattress. The one-size-fits-all dust ruffle disclosed herein eliminates the necessity of time-consuming dust ruffle placement which often requires two people. In the past, one person lifts the mattress while the second person positions the dust ruffle. Moreover, the one-size-fits-all also eliminates the necessity of multiple different dust ruffles to accommodate different mattress sizes. In this manner, dust ruffles can be switched from bed to bed without regard of the mattress size.