Patents by Inventor Jennifer Ong
Jennifer Ong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240150770Abstract: Compositions, methods and kits are provided that include an inhibitory oligonucleotide RNase inhibitor capable of inhibiting one or more types of RNase that coexist with biological samples or are introduced in the laboratory, thereby protecting RNA in the sample from degradation. More than one type of oligonucleotide RNase inhibitor may be combined in a mixture to inhibit a plurality of different RNases. Single oligonucleotides were identified to have inhibitory activity for a plurality of different RNases. The RNase oligonucleotide inhibitor may be immobilized on beads or other surface. It may be stored in a lyophilized form or in solution.Type: ApplicationFiled: November 15, 2023Publication date: May 9, 2024Applicant: New England Biolabs, Inc.Inventors: Nathan Tanner, Jennifer Ong, Esta Slayton, Lisa L. Maduzia, Salvatore V. Russello
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Patent number: 11827885Abstract: Compositions, methods and kits are provided that include an inhibitory oligonucleotide RNase inhibitor capable of inhibiting one or more types of RNase that coexist with biological samples or are introduced in the laboratory, thereby protecting RNA in the sample from degradation. More than one type of oligonucleotide RNase inhibitor may be combined in a mixture to inhibit a plurality of different RNases. Single oligonucleotides were identified to have inhibitory activity for a plurality of different RNases. The RNase oligonucleotide inhibitor may be immobilized on beads or other surface. It may be stored in a lyophilized form or in solution.Type: GrantFiled: March 19, 2021Date of Patent: November 28, 2023Assignee: New England Biolabs, Inc.Inventors: Nathan Tanner, Jennifer Ong, Esta Slayton, Lisa Maduzia, Salvatore V. Russello
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Patent number: 11713484Abstract: Providing herein, among other things, is a method comprising incubating a double-stranded nucleic acid having a nick with a nick translating activity, a ligase, and a nucleotide mix comprising at least one modified nucleotide, to generate a product comprising a patch of a newly synthesized strand of a duplex nucleic acid containing a plurality of modified nucleoside monophosphates that are at or adjacent to the site of the nick. In some embodiments, the method may be used to map damaged nucleoside monophosphates in a nucleic acid. Compositions and kits for use in performing the method are also provided.Type: GrantFiled: August 21, 2018Date of Patent: August 1, 2023Inventors: Kelly M. Zatopek, Vladimir Potapov, Jennifer Ong, Laurence Ettwiller, Lixin Chen, Thomas C. Evans, Jr., Andrew F. Gardner
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Publication number: 20220380741Abstract: The present disclosure relates, according to some embodiments, to systems, apparatus, compositions, methods, and workflows that include DNase I variants with desirable properties including, for example, salt tolerance. A DNase I variant, in some embodiments, may have an amino acid sequence that is at least 85% identical, at least 90% identical, at least 95% identical, and/or at least 98% identical to SEQ ID NO:1 and may be identical to SEQ ID NO:1 at one or more positions selected from the group of positions corresponding to L29, A35, D87, Q88, S94, P103, T108, P121, P132, A135, D145, E161, G172, P190, H208, and A224 of SEQ ID NO:1.Type: ApplicationFiled: May 27, 2021Publication date: December 1, 2022Applicant: New England Biolabs, Inc.Inventors: Heidi Crosby, Jennifer Ong, Ashley Luck, Eric J. Cantor, Vladimir Potapov
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Publication number: 20220275352Abstract: A bacteriophage RNA polymerase variant is provided. In some embodiments, the variant may have increased thermostability relative to the corresponding wild type bacteriophage RNA polymerase and/or wild type T7 RNA polymerase. Compositions, kits and methods that employ the variant are also provided.Type: ApplicationFiled: May 11, 2022Publication date: September 1, 2022Applicant: New England Biolabs, Inc.Inventors: Jennifer Ong, Vladimir Potapov, Kuo-Chan Hung, Haruichi Asahara, Shaorong Chong, George Tzertzinis
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Patent number: 11371028Abstract: Variants of the bacteriophage B103 DNA polymerase are described herein. The variant has improved properties, that include when compared to wild-type Phi29 DNA polymerase, at least one of the following: increased thermostability, improved reaction rate for DNA amplification, reduced background and a reduction of bias. Methods of using the DNA polymerase variant are also described herein.Type: GrantFiled: January 25, 2021Date of Patent: June 28, 2022Assignee: New England Biolabs, Inc.Inventors: Jennifer Ong, Nathan Tanner, Yinhua Zhang, Yanxia Bei, Vladimir Potapov
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Patent number: 11359184Abstract: A bacteriophage RNA polymerase variant is provided. In some embodiments, the variant may have increased thermostability relative to the corresponding wild type bacteriophage RNA polymerase and/or wild type T7 RNA polymerase. Compositions, kits and methods that employ the variant are also provided.Type: GrantFiled: November 11, 2019Date of Patent: June 14, 2022Assignee: New England Biolabs, Inc.Inventors: Jennifer Ong, Vladimir Potapov, Kuo-Chan Hung, Haruichi Asahara, Shaorong Chong, George Tzertzinis
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Publication number: 20210317496Abstract: Methods and compositions for capping RNA in an in vitro transcription mixture are provided that include a thermostable RNA polymerase variant and a cap analog such that when a DNA template is added to the mixture, and the mixture is then incubated under conditions for in vitro transcription, capped RNA is produced.Type: ApplicationFiled: June 24, 2021Publication date: October 14, 2021Applicant: New England Biolabs, Inc.Inventors: Bijoyita Roy, Jennifer Ong
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Patent number: 11072808Abstract: Methods and compositions for capping RNA in an in vitro transcription mixture are provided that include a thermostable RNA polymerase variant and a cap analog such that when a DNA template is added to the mixture, and the mixture is then incubated under conditions for in vitro transcription, capped RNA is produced.Type: GrantFiled: October 4, 2018Date of Patent: July 27, 2021Assignee: New England Biolabs, Inc.Inventors: Bijoyita Roy, Jennifer Ong
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Publication number: 20210155909Abstract: Variants of the bacteriophage B103 DNA polymerase are described herein. The variant has improved properties, that include when compared to wild-type Phi29 DNA polymerase, at least one of the following: increased thermostability, improved reaction rate for DNA amplification, reduced background and a reduction of bias. Methods of using the DNA polymerase variant are also described herein.Type: ApplicationFiled: January 25, 2021Publication date: May 27, 2021Applicant: New England Biolabs, Inc.Inventors: Jennifer Ong, Nathan Tanner, Yinhua Zhang, Yanxia Bei, Vladimir Potapov
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Patent number: 10934533Abstract: Variants of the bacteriophage B103 DNA polymerase are described herein. The variant has improved properties, that include when compared to wild-type Phi29 DNA polymerase, at least one of the following: increased thermostability, improved reaction rate for DNA amplification, reduced background and a reduction of bias. Methods of using the DNA polymerase variant are also described herein.Type: GrantFiled: October 10, 2019Date of Patent: March 2, 2021Assignee: New England Biolabs, Inc.Inventors: Jennifer Ong, Nathan Tanner, Yinhua Zhang, Yanxia Bei, Vladimir Potapov
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Patent number: 10837009Abstract: Mutant bacteriophage DNA ligases that have increased tolerance to salt and/or heat is provided. Methods, compositions and kits that employ the same are also provided.Type: GrantFiled: December 20, 2018Date of Patent: November 17, 2020Assignee: New England Biolabs, Inc.Inventors: Jennifer Ong, Gregory Lohman, Aine Quimby, Vladimir Potapov, John M. Pryor
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Publication number: 20200325533Abstract: Providing herein, among other things, is a method comprising incubating a double-stranded nucleic acid having a nick with a nick translating activity, a ligase, and a nucleotide mix comprising at least one modified nucleotide, to generate a product comprising a patch of a newly synthesized strand of a duplex nucleic acid containing a plurality of modified nucleoside monophosphates that are at or adjacent to the site of the nick. In some embodiments, the method may be used to map damaged nucleoside monophosphates in a nucleic acid. Compositions and kits for use in performing the method are also provided.Type: ApplicationFiled: August 21, 2018Publication date: October 15, 2020Applicant: New England Biolabs, Inc.Inventors: Kelly M. Zatopek, Vladimir Potapov, Jennifer Ong, Laurence Ettwiller, Lixin Chen, Thomas C. Evans, Jr., Andrew F. Gardner
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Publication number: 20200123513Abstract: A bacteriophage RNA polymerase variant is provided. In some embodiments, the variant may have increased thermostability relative to the corresponding wild type bacteriophage RNA polymerase and/or wild type T7 RNA polymerase. Compositions, kits and methods that employ the variant are also provided.Type: ApplicationFiled: November 11, 2019Publication date: April 23, 2020Applicant: New England Biolabs, Inc.Inventors: Jennifer Ong, Vladimir Potapov, Kuo-Chan Hung, Haruichi Asahara, Shaorong Chong, George Tzertzinis
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Publication number: 20200109425Abstract: Methods and compositions for capping RNA in an in vitro transcription mixture are provided that include a thermostable RNA polymerase variant and a cap analog such that when a DNA template is added to the mixture, and the mixture is then incubated under conditions for in vitro transcription, capped RNA is produced.Type: ApplicationFiled: October 4, 2018Publication date: April 9, 2020Applicant: New England Biolabs, Inc.Inventors: Bijoyita Roy, Jennifer Ong
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Patent number: 10519431Abstract: A bacteriophage RNA polymerase variant is provided. In some embodiments, the variant may have increased thermostability relative to the corresponding wild type bacteriophage RNA polymerase and/or wild type T7 RNA polymerase. Compositions, kits and methods that employ the variant are also provided.Type: GrantFiled: May 12, 2017Date of Patent: December 31, 2019Assignee: New England Biolabs, Inc.Inventors: Jennifer Ong, Vladimir Potapov, Kuo-Chan Hung, Haruichi Asahara, Shaorong Chong, George Tzertzinis
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Publication number: 20190024061Abstract: A bacteriophage RNA polymerase variant is provided. In some embodiments, the variant may have increased thermostability relative to the corresponding wild type bacteriophage RNA polymerase and/or wild type T7 RNA polymerase. Compositions, kits and methods that employ the variant are also provided.Type: ApplicationFiled: January 12, 2017Publication date: January 24, 2019Applicant: New England Biolabs, Inc.Inventors: Jennifer Ong, Shaorong Chong, Haruichi Asahara, Kuo-Chan Hung, Vladimir Potapov, George Tzertzinis
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Patent number: 9932567Abstract: A mutant MMLV reverse transcriptase that may have an improvement in one or more properties is provided. For example, the present reverse transcriptase is believed to be more efficient relative to other commercially available MMLV reverse transcriptase variants, particularly for templates with a higher GC content.Type: GrantFiled: January 13, 2017Date of Patent: April 3, 2018Assignee: New England Biolabs, Inc.Inventors: Yan Xu, Jennifer Ong, Shengxi Guan, Nicole Nichols
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Publication number: 20180087036Abstract: A mutant MMLV reverse transcriptase that may have an improvement in one or more properties is provided. For example, the present reverse transcriptase is believed to be more efficient relative to other commercially available MMLV reverse transcriptase variants, particularly for templates with a higher GC content.Type: ApplicationFiled: January 13, 2017Publication date: March 29, 2018Applicant: New England Biolabs, Inc.Inventors: Yan Xu, Jennifer Ong, Shengxi Guan, Nicole Nichols
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Publication number: 20170247670Abstract: A bacteriophage RNA polymerase variant is provided. In some embodiments, the variant may have increased thermostability relative to the corresponding wild type bacteriophage RNA polymerase and/or wild type T7 RNA polymerase. Compositions, kits and methods that employ the variant are also provided.Type: ApplicationFiled: May 12, 2017Publication date: August 31, 2017Applicant: New England Biolabs, Inc.Inventors: Jennifer Ong, Vladimir Potapov, Kuo-Chan Hung, Haruichi Asahara, Shaorong Chong, George Tzertzinis