Abstract: Method for the rapid detection of the presence or absence of Mycobacterium tuberculosis (MTB) and other members of the MTB-complex in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the esxJ gene, along with kits are provided that are designed for the detection of MTB.

Abstract: Methods for the rapid detection of the presence or absence of mecC-containing Staphylococcus aureus (mecC-MRSA) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the genes for mecC-MRSA, along with kits are provided that are designed for the detection of mecC-MRSA.

Abstract: The present invention relates to methods for the rapid detection of the presence or absence of Staphylococcus aureus in a biological or nonbiological sample. The present invention includes methods of detection comprising performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, the present invention relates to primers, probes, and kits that are designed for the detection of Staphylococcus aureus.

Abstract: The present invention relates to methods for the rapid detection of the presence or absence of Staphylococcus aureus in a biological or nonbiological sample. The present invention includes methods of detection comprising performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, the present invention relates to primers, probes, and kits that are designed for the detection of Staphylococcus aureus.

Abstract: The present invention relates to methods for the rapid detection of the presence or absence of Staphylococcus aureus in a biological or nonbiological sample. The present invention includes methods of detection comprising performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, the present invention relates to primers, probes, and kits that are designed for the detection of Staphylococcus aureus.

Abstract: Photoblocked probes are disclosed including a specific hydrolysis probe having a first nucleic acid sequence complementary to a target having a second nucleic acid sequence, a first and a second interactive labels, a 5? end and a 3? end, and one or more photocleavable moieties coupled to one or more nucleotides of the specific hydrolysis probe, wherein the photocleaveable moiety interferes with the hybridization of the specific hydrolysis probe with the region of the amplification product. Also disclosed are PCR methods for the detection of the presence or absence of a target nucleic acid in a sample utilizing the photoblocked probes, as well as kits.

Abstract: A pre-analytic reagent is provided for liquefaction of a sputum sample and complete inactivation of one or more Mycobacteria, e.g., Mycobacterium tuberculosis (MTB) suspected of being contained in the sputum sample at room temperature. The pre-analytic reagent may include one or more of each of: a chaotropic agent, a chelator, a reducing agent, a detergent, and acetic acid. The pre-analytic reagent can be configured to be effective to liquefy the sputum sample and completely inactivate the Mycobacteria suspected to be contained therein.

Abstract: Method for the rapid detection of the presence or absence of Mycobacterium tuberculosis (MTB) and other members of the MTB-complex in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the esxJ gene, along with kits are provided that are designed for the detection of MTB.

Abstract: Methods for the rapid detection of the presence or absence of mecC-containing Staphylococcus aureus (mecC-MRSA) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the genes for mecC-MRSA, along with kits are provided that are designed for the detection of mecC-MRSA.

Abstract: Photoblocked probes are disclosed including a specific hydrolysis probe having a first nucleic acid sequence complementary to a target having a second nucleic acid sequence, a first and a second interactive labels, a 5? end and a 3? end, and one or more photocleavable moieties coupled to one or more nucleotides of the specific hydrolysis probe, wherein the photocleaveable moiety interferes with the hybridization of the specific hydrolysis probe with the region of the amplification product. Also disclosed are PCR methods for the detection of the presence or absence of a target nucleic acid in a sample utilizing the photoblocked probes, as well as kits.

Abstract: The present invention relates to methods for the rapid detection of the presence or absence of Staphylococcus aureus in a biological or nonbiological sample. The present invention includes methods of detection comprising performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, the present invention relates to primers, probes, and kits that are designed for the detection of Staphylococcus aureus.

Abstract: The present invention relates to methods for the rapid detection of the presence or absence of Staphylococcus aureus in a biological or nonbiological sample, The present invention includes methods of detection comprising performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, the present invention relates to primers, probes, and kits that are designed for the detection of Staphylococcus aureus.

Abstract: The present invention relates to methods for the rapid detection of the presence or absence of Staphylococcus aureus in a biological or nonbiological sample. The present invention includes methods of detection comprising performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, the present invention relates to primers, probes, and kits that are designed for the detection of Staphylococcus aureus.

Abstract: The present invention relates to methods for the rapid detection of the presence or absence of Staphylococcus aureus in a biological or nonbiological sample. The present invention includes methods of detection comprising performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, the present invention relates to primers, probes, and kits that are designed for the detection of Staphylococcus aureus.

Abstract: Methods for the detection of the presence or absence of a single nucleotide polymorphism (SNP) in a target nucleic acid in a biological or non-biological sample are described. The methods can include performing an amplifying step using primers, a hybridizing step utilizing a melting probe, and a detecting step, wherein a decreasing shift in the predefined melting temperature of the melting probe is indicative of the presence of the SNP in the sample and wherein the absence of a decreasing shift in the predefined melting temperature of the melting probe is indicative of the absence of the SNP in the sample.

Abstract: The present invention is a method of separating nucleic acids using a solid phase capable of binding nucleic acids, such as magnetic glass particles, where binding of nucleic acids to the solid phase is enhanced by the presence of an ethylene-amine compound. The invention further includes a reaction mixture for isolating nucleic acids containing an ethylene-amine compound and kits for carrying out the method.

Abstract: The present invention relates to methods for the rapid detection of the presence or absence of Staphylococcus aureus in a biological or nonbiological sample. The present invention includes methods of detection comprising performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, the present invention relates to primers, probes, and kits that are designed for the detection of Staphylococcus aureus.

Abstract: The invention is a method of amplifying nucleic acids by synthesizing an engineered amplicon containing the sequence of interest, but omitting intervening sequences present in the template molecule. The method utilizes an “amplicon bridge” incorporated into the amplification primers. The length and content of the desired amplicon can be chosen by the operator and can contain unique regions for probe binding.

Abstract: The present invention is a method of separating nucleic acids using a solid phase capable of binding nucleic acids, such as magnetic glass particles, where binding of nucleic acids to the solid phase is enhanced by the presence of an ethylene-amine compound. The invention further includes a reaction mixture for isolating nucleic acids containing an ethylene-amine compound and kits for carrying out the method.