Patents by Inventor Jens FRAUENFELD
Jens FRAUENFELD has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220270707Abstract: The invention is directed to a method for studying or identifying a biologically active agent that binds to a membrane protein, the method comprising: (i) obtaining one or more 2D and/or 3D structures of a saposin lipoprotein particle comprising the membrane protein, (ii) modeling the binding of said biologically active agent to said membrane protein present in said saposin lipoprotein particle using said one or more 2D and/or 3D structures and/or resolving the binding sites and interactions between said biologically active agent and said membrane protein in the 2D and/or 3D structure.Type: ApplicationFiled: May 6, 2022Publication date: August 25, 2022Applicant: Salipro Biotech ABInventors: Jens FRAUENFELD, Robin LÖVING
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Publication number: 20220192982Abstract: The invention relates to a process for preparing saposin lipoprotein particles, comprising a saposin-like protein, lipids and optionally a hydrophobic agent wherein the saposin-like protein or the hydrophobic agent is selectively bound to a support to allow the self-assembly of the saposin lipoprotein particles. The process of the invention comprises the step of a.) providing the hydrophobic agent and lipids, b. 1)/b.2 contacting the hydrophobic agent or the saposin-like protein with a support that is capable of selectively binding either of the two molecules to the support, c.1)/c.2) contacting the support-bound particle components with the remaining particle components, either the saposin-like protein or the hydrophobic agent, to allow for the self-assembly of the saposin lipoprotein particle on the support and d.) optionally eluting the support-bound saposin lipoprotein particles.Type: ApplicationFiled: April 15, 2020Publication date: June 23, 2022Applicant: Salipro Biotech ABInventors: Robin LÖVING, Jens FRAUENFELD
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Patent number: 11346843Abstract: The invention is directed to a process for preparing a library of saposin lipoprotein particles, wherein the particles comprise membrane components from a cell or an organelle membrane and a lipid binding polypeptide that is a saposin-like protein belonging to the SAPLIP family of lipid interacting proteins or a derivative form thereof, wherein the process comprises the steps of a) providing a mixture of crude membrane vesicles obtained from a cell or an organelle membrane; b) contacting the mixture of step a) with the lipid binding polypeptide in a liquid environment; and c) allowing for self-assembly of the particles. The invention also provides a process for preparing a purified saposin lipoprotein particle comprising the steps of preparing a library according to the process described above and the additional step of f) purifying the saposin lipoprotein particle from the library.Type: GrantFiled: August 21, 2017Date of Patent: May 31, 2022Assignee: Salipro Biotech ABInventors: Jens Frauenfeld, Robin Löving
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Publication number: 20190204337Abstract: The invention is directed to a process for preparing a library of saposin lipoprotein particles, wherein the particles comprise membrane components from a cell or an organelle membrane and a lipid binding polypeptide that is a saposin-like protein belonging to the SAPLIP family of lipid interacting proteins or a derivative form thereof, wherein the process comprises the steps of a) providing a mixture of crude membrane vesicles obtained from a cell or an organelle membrane; b) contacting the mixture of step a) with the lipid binding polypeptide in a liquid environment; and c) allowing for self-assembly of the particles. The invention also provides a process for preparing a purified saposin lipoprotein particle comprising the steps of preparing a library according to the process described above and the additional step of f) purifying the saposin lipoprotein particle from the library.Type: ApplicationFiled: August 21, 2017Publication date: July 4, 2019Applicant: Salipro Blotech ABInventors: Dr. Jens Frauenfeld, Robin LÖVING
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Patent number: 10159729Abstract: The invention refers to a method for producing an antigen comprising at least one hydrophobic or partially hydrophobic antigen molecule from a virus, a bacterium, fungus, protozoan, parasite, a human neoplastic cell or an animal neoplastic, tumor or 5 cancer cell, the method comprising the steps of providing a virus, or cell comprising an antigen molecule, purifying the cell comprising the antigen molecule, solubilizing the antigen molecule in a solubilizing agent that preserves an intact antigen molecule upon solubilization and reconstituting the antigen molecule in a lipid-binding polypeptide that provides a lipid membrane mimicking environment and a reconstituted antigen particle 10 obtained by this method.Type: GrantFiled: September 12, 2014Date of Patent: December 25, 2018Assignee: Sallpro Biotech ABInventors: Robin Löving, Jens Frauenfeld, Gunilla Karlsson Hedestam, Henrik Garoff, Mathilda Sjöberg
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Publication number: 20180236099Abstract: The invention provides a nanoscale particle comprising a lipid binding polypeptide, lipids and a hydrophobic agent, wherein the hydrophobic agent is different from the lipids, and wherein the lipid binding polypeptide is a saposin-like protein or a derivative or truncated form thereof. The invention further provides a process for preparing a particle comprising a saposin-like protein or a derivative or truncated form thereof and lipids comprising the step of (a) contacting the saposin-like protein or a derivative or truncated form thereof with solubilized lipids in a liquid environment and (b) allowing for the self-assembly of the particle at a pH of from 5.0 to 10:0. In addition, pharmaceutical compositions and methods for using the particle of the invention are described.Type: ApplicationFiled: January 10, 2018Publication date: August 23, 2018Inventor: Jens Frauenfeld
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Patent number: 9884128Abstract: The invention provides a nanoscale particle comprising a lipid binding polypeptide, lipids and a hydrophobic agent, wherein the hydrophobic agent is different from the lipids, and wherein the lipid binding polypeptide is a saposin-like protein or a derivative or truncated form thereof. The invention further provides a process for preparing a particle comprising a saposin-like protein or a derivative or truncated form thereof and lipids comprising the step of (a) contacting the saposin-like protein or a derivative or truncated form thereof with solubilized lipids in a liquid environment and (b) allowing for the self-assembly of the particle at a pH of from 5.0 to 10.0.Type: GrantFiled: December 12, 2013Date of Patent: February 6, 2018Inventor: Jens Frauenfeld
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Publication number: 20160220664Abstract: The invention refers to a method for producing an antigen comprising at least one hydrophobic or partially hydrophobic antigen molecule from a virus, a bacterium, fungus, protozoan, parasite, a human neoplastic cell or an animal neoplastic, tumour or 5 cancer cell, the method comprising the steps of providing a virus, or cell comprising an antigen molecule, purifying the cell comprising the antigen molecule, solubilizing the antigen molecule in a solubilizing agent that preserves an intact antigen molecule upon solubilisation and reconstituting the antigen molecule in a lipid-binding polypeptide that provides a lipid membrane mimicking environment and a reconstituted antigen particle 10 obtained by this method.Type: ApplicationFiled: September 12, 2014Publication date: August 4, 2016Inventors: Robin Löving, Jens Frauenfeld, Gunilla Karlsson Hedestam, Henrik Garoff, Mathilda Sjöberg
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Publication number: 20160082125Abstract: The invention provides a nanoscale particle comprising a lipid binding polypeptide, lipids and a hydrophobic agent, wherein the hydrophobic agent is different from the lipids, and wherein the lipid binding polypeptide is a saposin-like protein or a derivative or truncated form thereof. The invention further provides a process for preparing a particle comprising a saposin-like protein or a derivative or truncated form thereof and lipids comprising the step of (a) contacting the saposin-like protein or a derivative or truncated form thereof with solubilized lipids in a liquid environment and (b) allowing for the self-assembly of the particle at a pH of from 5.0 to 10.0.Type: ApplicationFiled: December 12, 2013Publication date: March 24, 2016Inventor: Jens FRAUENFELD