Abstract: An in vitro testing platform for the study of metabolic liver disease and related therapeutic strategies, composed of an artificial spheroidal microtissue comprising at least hepatocytes and hepatic stellate cells, and at least one type of hepatic inflammatory cells and further medium and reagents capable to establish and simulate different stages of metabolic liver disease and their progression and testing preventive and therapeutic strategies by pharmacological and/or dietary interventions.

Abstract: The present invention relates to an in vitro testing platform for the study of metabolic liver disease and related therapeutic strategies, composed of an artificial spheroidal microtissue comprising at least hepatocytes and hepatic stellate cells, and at least one type of hepatic inflammatory cells and further medium and reagents capable to establish and simulate different stages of metabolic liver disease and their progression and testing preventive and therapeutic strategies by pharmacological and/or dietary interventions.

Abstract: The present invention relates to an in vitro tumor relapse assay method, which method comprises providing a 3-dimensional cell culture or tissue comprising immortalized, malignant, cancerous and/or neoplastic cells, exposing the 3-dimensional cell culture or tissue to at least one anti-cancer agent, interrupting the exposure and determining the effect of said exposure and interruption on the 3-dimensional cell culture or tissue.

Abstract: The present invention relates to a method of preparing cells for 3D tissue culture, which method comprises the steps of plating the cells on a suitable surface, optionally, checking for their capability to adhere to said surface, discarding the cells which have not adhered to said surface, detaching the adhered cells and transferring them into a 3D tissue culture process.