Abstract: The present invention related to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing and secreting different recombinant proteins. The improved ability is obtained by disruption in intracellular transport between the Golgi and the endosome. In particular embodiments, the disruption is achieved by downregulation or deletion of the gene encoding a Tda3p homolog. The fungal cell and method of the invention would allow for large-scale production of recombinant proteins in fungal cells.

Abstract: The invention relates to production of polyamine conjugates in yeast cells that are capable of producing at least one polyamine. The yeast cells also comprise a polyamine:glutathione ligase encoding gene and at least one polyamine synthase encoding gene but lacks a polyamine oxidase encoding gene or comprises a disrupted polyamine oxidase encoding gene. The yeast cells are capable of producing various polyamine-glutathione conjugates.

Abstract: The invention relates to production of polyamine analogs in yeast cells that are capable of producing at least one polyamine. The yeast cells also comprise a 4-coumarate:CoA ligase encoding gene, at least one polyamine N-acyltransferase gene and at least one polyamine synthase encoding gene but lacks a polyamine oxidase encoding gene or comprises a disrupted polyamine oxidase encoding gene. The yeast cells are capable of producing mono- and/or multi-substituted N-acylated polyamines.

Abstract: The present invention relates to a method of screening for cancer in a subject, said method comprising determining the level and/or chemical composition of the protein-free fraction of one or both of the glycosaminoglycans (GAGs) chondroitin sulfate (CS) and heparan sulfate (HS) in a body fluid sample, wherein said sample has been obtained from said subject.

Abstract: A maritime evacuation system to be installed on a vessel or offshore facility comprises a storage unit to be installed on the vessel or offshore facility, at least two survival crafts, each having a hull, predominantly made of non-rigid inflatable tubes and one or more shells, the survival crafts being configured to be stored in the storage unit in a deflated state, each survival craft comprises at least two engine powered propulsion means, the maritime evacuation system further comprising a central control unit being operatively connected with each engine powered propulsion means, wherein the central control unit is configured to observe the condition of and test the readiness of each engine powered propulsion means at predetermined intervals so that it is continuously monitored if the maritime evacuation system meets the requirements for being ready for rescue.

Abstract: The present invention related to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing and secreting different recombinant proteins. The improved ability is obtained by disruption in intracellular transport between the Golgi and the endosome. In particular embodiments, the disruption is achieved by downregulation or deletion of the gene encoding a Tda3p homolog. The fungal cell and method of the invention would allow for large-scale production of recombinant proteins in fungal cells.

Abstract: A survival craft for maritime evacuation of passengers and crew members from a vessel or an offshore facility is disclosed. The survival craft has a hull, predominantly made of non-rigid inflatable tubes and one or more shells, the hull has a length, a width and a centerline, a bow and a stern. The survival craft further comprises a first pair of engine-powered propulsion means arranged at the stern on opposite sides of the centerline, and a second pair of engine-powered propulsion means arranged at the bow on opposite sides of the centerline, each engine-powered propulsion means has a propeller or a water jet, wherein a horizontal angle of each propeller or water jet of each engine-powered propulsion means can be independently set in relation to the centerline.

Abstract: A fungal cell capable of producing high levels of fatty acids and fatty acid-derived products comprises at least one modification to the endogenous fatty acid metabolism.

Abstract: A fungal cell is capable of producing high levels of fatty acids and fatty acid-derived products. The fungal cell comprises at least one modification to the endogenous fatty acid metabolism.

Abstract: The present invention relates to protease variants and methods for obtaining protease variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: The invention relates to a W/O emulsion for impregnating nonwoven containing 10-60% by weight of vegetable oil, 0.1-25% by weight of emollients, 10 to 50% by weight of water, a lipophilic emulsifier, an inorganic salt as well as at least one antioxidant, wherein the emulsifier is polyglyceryl-4-diisostearate/polyhydroxystearate/sebacate and the viscosity of the emulsion is 250-600 mPa·s, determined using a DIN measuring system 33 according to ISO 53019 at a temperature of 293.15 K and at a rotation speed of 500 r/min.

Abstract: The present invention relates to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing different fatty acids and specifically fatty acid ethyl esters (FAEE), the main components of biodiesel. An increased in fatty acid production, and hence in FAEE, is obtained in the first place by expressing different heterologous polypeptides in combination with the down-regulation, attenuation, deletion or over-expression of specially selected genes, wherein said genes encode enzymes involved in the fatty acids synthesizing pathway, fatty acid consuming pathways, carbohydrate biosynthesis pathways or enzyme acting as wax ester transporters or a combination thereof. The methods and products of the invention would allow large-scale production of FAEE with carbohydrates as the only externally-supplied substrate.

Abstract: The invention relates to engineering of acetyl-CoA metabolism in yeast and in particular to production of acetyl-CoA in a non-ethanol producing yeast lacking endogenous gene(s) encoding pyruvate decarboxylase and comprising a heterologous pathway for synthesis of cytosolic acetyl-CoA.

Abstract: A fungal cell is capable of producing high levels of fatty acids and fatty acid-derived products. The fungal cell comprises at least one modification to the endogenous fatty acid metabolism.

Abstract: The present invention relates to a method of screening for renal cell carcinoma in a subject, said method comprising determining the level and/or chemical composition of one or both of the glycosaminoglycans (GAGs) chondroitin sulfate (CS) and heparan sulfate (HS) in a body fluid sample, wherein said sample has been obtained from said subject.

Abstract: The invention relates to engineering of acetyl-CoA metabolism in yeast and in particular to production of acetyl-CoA in a non-ethanol producing yeast lacking endogenous gene(s) encoding pyruvate decarboxylase and comprising a heterologous pathway for synthesis of cytosolic acetyl-CoA.

Abstract: The invention relates to a W/O emulsion for impregnating nonwoven containing 10-60% by weight of vegetable oil, 0.1-25% by weight of emollients, 10 to 50% by weight of water, a lipophilic emulsifier, an inorganic salt as well as at least one antioxidant, wherein the emulsifier is polyglyceryl-4-diisostearate/polyhydroxystearate/sebacate and the viscosity of the emulsion is 250-600 mPa·s, determined using a DIN measuring system 33 according to ISO 53019 at a temperature of 293.15 K and at a rotation speed of 500 r/min.

Abstract: The present invention related to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing and secreting different recombinant proteins. The improved ability is obtained by disruption in intracellular transport between the Golgi and the endosome. In particular embodiments, the disruption is achieved by downregulation or deletion of the gene encoding a Tda3p homolog. The fungal cell and method of the invention would allow for large-scale production of recombinant proteins in fungal cells.

Abstract: The present invention generally related to a modified microbial cell capable of producing high levels of spermidine and/spermidine derivatives. The genetically modified microbial cell comprises at least one modification to native spermidine biosynthetic pathway via putrescine together with genes involved in the S-adenosylmethionine biosyntheticpathway.

Abstract: The present invention relates to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing different fatty acids and specifically fatty acid ethyl esters (FAEE), the main components of biodiesel. An increased in fatty acid production, and hence in FAEE, is obtained in the first place by expressing different heterologous polypeptides in combination with the down-regulation, attenuation, deletion or over-expression of specially selected genes, wherein said genes encode enzymes involved in the fatty acids synthesizing pathway, fatty acid consuming pathways, carbohydrate biosynthesis pathways or enzyme acting as wax ester transporters or a combination thereof. The methods and products of the invention would allow large-scale production of FAEE with carbohydrates as the only externally-supplied substrate.