Patents by Inventor Jens Sigurd Okkels
Jens Sigurd Okkels has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20120107852Abstract: Methods for producing polypeptide with altered immunogenicity or improved stability properties are disclosed. The methods involve a) expressing a diversified population of nucleotide sequences encoding a polypeptide of interest, b) screening the polypeptides expressed in step a) for function, immunogenicity and/or stability, c) selecting functional polypeptides having altered immunogenicity and/or increased stability, e.g. functional in vivo half-life as compared to the polypeptide of interest, and d) optionally subjecting the nucleotide sequence encoding the polypeptide selected in step c) to one or more repeated cycles of steps a)-c). In a further step the expressed polypeptides of step a) or c) can be conjugated to at least one non-polypeptide moiety.Type: ApplicationFiled: October 13, 2011Publication date: May 3, 2012Inventors: Torben Halkier, Anders Hjelholt Pedersen, Jens Sigurd Okkels, Kim Vilbour Andersen
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Publication number: 20090088559Abstract: Methods for producing polypeptide with altered immunogenicity or improved stability properties are disclosed. The methods involve a) expressing a diversified population of nucleotide sequences encoding a polypeptide of interest, b) screening the polypeptides expressed in step a) for function, immunogenicity and/or stability, c) selecting functional polypeptides having altered immunogenicity and/or increased stability, e.g. functional in vivo half-life as compared to the polypeptide of interest, and d) optionally subjecting the nucleotide sequence encoding the polypeptide selected in step c) to one or more repeated cycles of steps a)-c). In a further step the expressed polypeptides of step a) or c) can be conjugated to at least one non-polypeptide moiety.Type: ApplicationFiled: January 30, 2008Publication date: April 2, 2009Inventors: Torben Halkier, Anders Hjelholt Pedersen, Jens Sigurd Okkels, Kim Vilbour Andersen
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Patent number: 7157262Abstract: The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.Type: GrantFiled: August 30, 2002Date of Patent: January 2, 2007Assignee: Novozymes A/SInventors: Claus Crone Fuglsang, Jens Sigurd Okkels, Dorte Aaby Petersen, Shamkant Anant Patkar, Marianne Thellersen, Allan Svendsen, Kim Borch, John C. Royer, Titus Kretzschmar, Torben Halkier, Jesper Vind, Steen Troels Jorgensen
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Publication number: 20040014948Abstract: The invention relates to a single-chain oilgomeric polypeptide antagonist which binds to an extracellular ligand-binding domain of a cellular receptor of a type requiring binding of an oligomeric ligand to two or more receptor subunits to be activated, the polypeptide comprising at least two, typically structurally homologous, receptor-binding sites of which at least one is capable of binding to a ligand-binding domain of the cellular receptor and at least one is incapable of effectively binding to a ligand-binding domain of the cellular receptor, whereby the single-chain oligomeric polypeptide is capable of binding to the receptor, but incapable of activating the receptor; as well as to nucleotide sequences encoding such single-chain oligomeric polypeptides, expression vectors comprising such a nucleotide sequence, recombinant host cells comprising such a nucleotide sequence or expression vector, methods for producing the nucleotide sequences and polypeptides, pharmaceutical compositions comprising the singlType: ApplicationFiled: May 23, 2003Publication date: January 22, 2004Applicant: Maxygen ApSInventors: Torben Halkier, Hans Thalsgard Schambye, Jens Sigurd Okkels, Kim Vilbour Andersen, Torben Lauesgaard Nissen, Bobby Soni, Claus Bekker Jeppesen, Bart van den Hazel
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Publication number: 20040009165Abstract: A polypeptide selected from the group of lysosomal enzymes and lysosomal enzyme activators, comprising at least one introduced glycosylation site as compared to a corresponding parent enzyme or activator. By introducing additional glycosylation sites the resulting glycosylated lysosomal enzyme or activator obtains improved in vivo activity and thereby provides for improved treatment of lysosomal storage diseases.Type: ApplicationFiled: December 27, 2002Publication date: January 15, 2004Applicant: Maxygen ApSInventors: Jens Sigurd Okkels, Anne Dam Jensen, Torben Halkier, Rikke Bolding Jensen, Hans Thalsgard Schambye
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Publication number: 20030199069Abstract: The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.Type: ApplicationFiled: August 30, 2002Publication date: October 23, 2003Applicant: Novozymes A/SInventors: Claus Crone Fuglsang, Jens Sigurd Okkels, Dorte Aaby Petersen, Shamkant Anant Patkar, Marianne Thellersen, Allan Svendsen, Kim Borch, John C. Royer, Titus Kretzschmar, Torben Halkier, Jesper Vind, Steen Troels Jorgensen
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Publication number: 20030165996Abstract: Methods for producing polypeptide with altered immunogenicity or improved stability properties are disclosed.Type: ApplicationFiled: July 3, 2002Publication date: September 4, 2003Inventors: Torben Halkier, Anders Hjelholt Pedersen, Jens Sigurd Okkels, Kim Vilbour Andersen
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Publication number: 20030148441Abstract: The present invention relates to a method for preparing positive polypeptide variants by shuffling different nucleotide sequences of homologous DNA sequences by in vivo recombination comprising the steps of (a) forming at least one circular plasmid comprising a DNA sequence encoding a polypeptide, (b) opening said circular plasmid(s) within the DNA sequence(s) encoding the polypeptide(s), (c) preparing at least one DNA fragment comprising a DNA sequence homologous to at least a part of the polypeptide coding region on at least one of the circular plasmid(s), (d) introducing at least one of said opened plasmid(s), together with at least one of said homologous DNA fragment(s) covering full-length DNA sequences encoding said polypeptide(s) or parts thereof, into a recombination host cell, (e) cultivating said recombination host cell, and (f) screening for positive polypeptide variants.Type: ApplicationFiled: July 3, 2002Publication date: August 7, 2003Applicant: Novozymes A/SInventor: Jens Sigurd Okkels
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Publication number: 20030036181Abstract: Glycosylated polypeptides comprising the primary structure NH2—X—Pp—COOH, wherein X is a peptide addition comprising or contributing to a glycosylation site, and Pp is a polypeptide of interest or comprising the primary structure NH2-Px—X—Py-COOH, wherein Px is an N-terminal part of a polypeptide Pp of interest, Py is a C-terminal part of said polypeptide Pp, and X is a peptide addition comprising or contributing to a glycosylation site are provided. The glycosylated polypeptides possess improved properties as compared to the polypeptide of interest.Type: ApplicationFiled: June 29, 2001Publication date: February 20, 2003Inventors: Jens Sigurd Okkels, Anne Dam Jensen, Bart van den Hazel
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Patent number: 6495357Abstract: The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.Type: GrantFiled: January 14, 1998Date of Patent: December 17, 2002Assignee: Novozyme A/SInventors: Claus Crone Fuglsang, Jens Sigurd Okkels, Dorte Aaby Petersen, Shamkant Anant Patkar, Marianne Thellersen, Allan Svendsen, Kim Borch, John C. Royer, Titus Kretzschmar, Torben Halkier, Jesper Vind, Steen Troels Jorgensen
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Publication number: 20020127219Abstract: A polypeptide selected from the group of lysosomal enzymes and lysosomal enzyme activators, comprising at least one introduced glycosylation site as compared to a corresponding parent enzyme or activator. By introducing additional glycosylation sites the resulting glycosylated lysosomal enzyme or activator obtains improved in vivo activity and thereby provides for improved treatment of lysosomal storage diseases.Type: ApplicationFiled: December 29, 2000Publication date: September 12, 2002Inventors: Jens Sigurd Okkels, Anne Dam Jensen, Torben Halkier, Rikke Bolding Jensen, Hans Thalsgard Schambye
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Patent number: 6156552Abstract: The invention provides variant lipases with improved washing performance, including good performance in washing with a detergent having a high content of anionic surfactant at low washing temperature at a short washing time. More particularly, the invention relates to variants of the wild-type lipase from Pseudomonas sp. strain SD 705, deposited as FERM BP-4772.Type: GrantFiled: February 17, 1999Date of Patent: December 5, 2000Assignee: Novo Nordisk A/SInventors: Jens Sigurd Okkels, Shiro Fukuyama, Tomoko Matsui, Tadashi Yoneda
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Patent number: 5976855Abstract: The present invention relates to a method of preparing a variant of a parent lipolytic enzyme, comprising (a) subjecting a DNA sequence encoding the parent lipolytic enzyme to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and (c) screening for host cells expressing a mutated lipolytic enzyme which has a decreased dependance to calcium and/or an improved tolerance towards a detergent or a detergent component as compared to the parent lipolytic enzyme.Type: GrantFiled: August 22, 1996Date of Patent: November 2, 1999Assignee: Novo Nordisk A/SInventors: Allan Svendsen, Ib Groth Clausen, Jens Sigurd Okkels, Marianne Thellersen
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Patent number: 5892013Abstract: The present invention relates to lipase variants which exhibit improved properties, detergent compositions comprising said lipase variants, DNA constructs coding for said lipase variants, and methods of making said lipase variants.Type: GrantFiled: September 5, 1995Date of Patent: April 6, 1999Assignee: Novo Nordisk A/SInventors: Allan Svendsen, Shamkant Anant Patkar, Erik Gormsen, Ib Groth Clausen, Jens Sigurd Okkels, Marianne Thellersen
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Patent number: 5869438Abstract: The present invention relates to lipase variants which exhibit improved properties, detergent compositions comprising said lipase variants, DNA constructs coding for said lipase variants, and methods of making said lipase variants.Type: GrantFiled: June 7, 1995Date of Patent: February 9, 1999Assignee: Novo Nordisk A/SInventors: Allan Svendsen, Shamkant Anant Patkar, Erik Gormsen, Jens Sigurd Okkels, Marianne Thellersen