Abstract: Disclosed is a method for producing hyaluronidase or a variant thereof. Specifically, the method is capable of changing the N-glycan levels under culture conditions including a controlled concentration of glucose in the culture medium and a decreased culture temperature for a specific culture time period, thereby increasing the specific activity by 10% or more and improving the quality and production yield.

Abstract: An exhaust gas recirculation control apparatus includes: an EGR valve adjusting a flow rate of EGR gas recirculated from an exhaust manifold to an intake manifold; a manifold absolute pressure (MAP) sensor measuring pressure inside the intake manifold; a throttle valve controlling the amount of inflow air; an igniter spraying fuel; an acceleration pedal angular position sensor; a crank position sensor measuring engine RPM; a vehicle speed sensor measuring a speed of a vehicle; and a control portion receiving a pressure signal from the MAP sensor, calculating a ratio of the EGR gas for a total volume of the intake manifold by using pressure variance inside the intake manifold, calculating pressure of the EGR gas by multiplying the pressure of the intake manifold and the ratio of the EGR gas, and converting the pressure of the EGR gas to a flow rate of the EGR gas.

Abstract: An exhaust gas recirculation control apparatus includes: an EGR valve adjusting a flow rate of EGR gas recirculated from an exhaust manifold to an intake manifold; a manifold absolute pressure (MAP) sensor measuring pressure inside the intake manifold; a throttle valve controlling the amount of inflow air; an igniter spraying fuel; an acceleration pedal angular position sensor; a crank position sensor measuring engine RPM; a vehicle speed sensor measuring a speed of a vehicle; and a control portion receiving a pressure signal from the MAP sensor, calculating a ratio of the EGR gas for a total volume of the intake manifold by using pressure variance inside the intake manifold, calculating pressure of the EGR gas by multiplying the pressure of the intake manifold and the ratio of the EGR gas, and converting the pressure of the EGR gas to a flow rate of the EGR gas.

Abstract: The present invention relates to a novel YlMPO1 gene which plays an important role in mannosylphosphorylation of an industrial yeast Yarrowia lipolytica, and to a method for preparing a host system capable of producing recombinant glycoproteins free of mannosylphosphate by disruption of the gene. The mannosylphosphorylation is suppressed by the disruption of YlMPO1 gene according to the present invention, thereby achieving humanization of glycosylation pathway of Yarrowia lipolytica.

Abstract: The present invention relates to a novel YlMPOl gene which plays an important role in mannosylphosphorylation of an industrial yeast Yarrowia lipolytica, and to a method for preparing a host system capable of producing recombinant glycoproteins free of mannosylphosphate by disruption of the gene. The mannosylphosphorylation is suppressed by the disruption of YlMPOl gene according to the present invention, thereby achieving humanization of glycosylation pathway of Yarrowia lipolytica.