Abstract: Provided are methods for determining susceptibility, resistance or tolerance to Mycobacterium avium subspecies paratuberculosis (Map) infection, comprising: determining, using a biological sample from a mammalian subject, a presence or absence of at least one EDN2 gene mutation, or the genotype of at least one EDN2 gene single nucleotide polymorphism (SNP) selected from the SNP group consisting of SNP105 (nucleotide position 105,288,174 on BTA3 (UMD 3.1)), SNP208 (nucleotide position 105,298,664 on BTA3 (UMD 3.1)), and SNP272 (nucleotide position 105,305,070 by on BTA3 (UMD 3.1)), that segregates with resistance and/or tolerance to Map tissue infection; and determining, based thereon, at least one of susceptibility, resistance or tolerance of the mammalian subject to Map infection. Additionally provided are mammals (e.g., bovine) derived culling, selecting, or selective breeding, based on disclosed methods, and methods for procuring mammals.

Abstract: Provided are methods for determining susceptibility, resistance or tolerance to Mycobacterium avium subspecies paratuberculosis (Map) infection, comprising: determining, using a biological sample from a mammalian subject, a presence or absence of at least one EDN2 gene mutation, or the genotype of at least one EDN2 gene single nucleotide polymorphism (SNP) selected from the SNP group consisting of SNP105 (nucleotide position 105,288,174 on BTA3 (UMD 3.1)), SNP208 (nucleotide position 105,298,664 on BTA3 (UMD 3.1)), and SNP272 (nucleotide position 105,305,070 by on BTA3 (UMD 3.1)), that segregates with resistance and/or tolerance to Map tissue infection; and determining, based thereon, at least one of susceptibility, resistance or tolerance of the mammalian subject to Map infection. Additionally provided are mammals (e.g., bovine) derived culling, selecting, or selective breeding, based on disclosed methods, and methods for procuring mammals.

Abstract: Provided are methods for determining resistance or tolerance of a subject to Mycobacterium avium subspecies paratuberculosis (Map) infection, comprising determining, a presence or absence of a mutation, or the genotype of a single nucleotide polymorphism (SNP) within Map infection linkage disequilibrium region SEQ ID NO:46 that segregates with resistance or tolerance to Map infection, or determining a presence or absence of a mutation or a genotype of a SNP within a Map infection linkage disequilibrium region (SEQ ID NO:1) that is in linkage disequilibrium therewith, and determining, based thereon, susceptibility, resistance or tolerance of the subject to Map infection. In particular aspects, a presence or absence of a mutation, or the genotype of a single nucleotide polymorphism (SNP) within at least one gene selected from the group consisting of HIVEP3; EDN2; and LOC521287 that segregates with resistance or tolerance to Map tissue infection is determined.