Abstract: Apparatus and methods for detecting, characterizing, and compensating motion-related error of moving micro-entities are described. Motion-related error may occur in streams of moving micro-entities, and may represent a deviation in and expected arrival time or an uncertainty in position of a micro-entity within the stream. Motion-related error of micro-entities is observed in a flow cytometer, e.g., as pulse jitter, and is found to have a functional dependence on a parameter of the system. The pulse jitter can be compensated, according to one embodiment, by adjusting data acquisition observation windows. For the flow cytometer, a reduction of pulse jitter can improve measurement accuracy, resolution of doublets, system throughput, and enable an increase in an interrogation region for probing the micro-entities.

Abstract: Apparatus and methods for detecting and characterizing motion-related error of moving micro-entities are described. Motion-related error may occur in streams of moving micro-entities, and may represent a deviation in and expected arrival time or an uncertainty in position of a micro-entity. Motion-related error of micro-entities is observed in a flow cytometer, e.g., as pulse jitter, and is found to have a functional dependence on a parameter related to a system clock. The motion-related error may be characterized by correlating measurements of micro-entities moving within a fluid stream.

Abstract: Apparatus and methods for detecting, characterizing, and compensating motion-related error of moving micro-entities are described. Motion-related error may occur in streams of moving micro-entities, and may represent a deviation in and expected arrival time or an uncertainty in position of a micro-entity within the stream. Motion-related error of micro-entities is observed in a flow cytometer, e.g., as pulse jitter, and is found to have a functional dependence on a parameter of the system. The pulse jitter can be compensated, according to one embodiment, by adjusting data acquisition observation windows. For the flow cytometer, a reduction of pulse jitter can improve measurement accuracy, resolution of doublets, system throughput, and enable an increase in an interrogation region for probing the micro-entities.

Abstract: A system and method for the measurement of multiple fluorescence emissions in a flow cytometry system is disclosed where each excitation light source is modulated with a different frequency. A single detector is used to collect the fluorescent emissions excited by all light sources, and the emissions are segregated using Fourier Transform techniques. Systems and methods for the correction of inter-beam coincidence are also disclosed.

Abstract: A system and method for the measurement of multiple fluorescence emissions in a flow cytometry system is disclosed where each excitation light source is modulated with a different frequency. A single detector is used to collect the fluorescent emissions excited by all light sources, and the emissions are segregated using Fourier Transform techniques. Systems and methods for the correction of inter-beam coincidence are also disclosed.

Abstract: A system and method for the measurement of multiple fluorescence emissions in a flow cytometry system is disclosed where each excitation light source is modulated with a different frequency. A single detector is used to collect the fluorescent emissions excited by all light sources, and the emissions are segregated using Fourier Transform techniques. Systems and methods for the correction of inter-beam coincidence are also disclosed.

Abstract: A system and method for the measurement of multiple fluorescence emissions in a flow cytometry system is disclosed where each excitation light source is modulated with a different frequency. A single detector is used to collect the fluorescent emissions excited by all light sources, and the emissions are segregated using Fourier Transform techniques. Systems and methods for the correction of inter-beam coincidence are also disclosed.

Abstract: A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.