Abstract: Methods and compositions for de novo and in vivo synthesis of soft tissue in predefined shape and dimensions from adult mesenchymal stem cells (MSCs) within a biocompatible scaffold are described. Scaffolds are implanted in vivo in a host animal and fabricated therein, or maintained ex vivo. Inducing angiogenesis enhances success of soft tissue implants.

Abstract: This invention relates to the synthesis of new tooth or bone from an encapsulated biological agent such as a growth factor or stem cells contained within a hollow and porous biocompatible vehicle such as a titanium implant placed (implanted) within the body of a host animal such as a human.

Abstract: The in vivo synthesis of connective tissue by fibroblast or fibroblast precursor cells ensconced within a biocompatible scaffold is disclosed. The cells are preferably present in a biocompatible scaffold such as gelatin and placed between two other biocompatible scaffolds such as collagen sponges soaked with a collagenic amount of a member of the TGF-? family of proteins. This composition is then implanted in a host to produce cranial sutures, periodontal ligament or other fibrous tissue structures in vivo.

Abstract: De novo organogenesis of a joint or portion thereof by osteochondral constructs comprising adult mesenchymal stem cells (MSCs) encapsulated on a scaffold is disclosed. MSCs-derived chondrogenic and osteogenic cells can be loaded in hydrogel monomer suspensions in distinct stratified and yet integrated layers that are sequentially photopolymerized in a mold. Constructs can be then implanted in vivo in a host and fabricated therein or, alternatively, the constructs can be incubated ex vivo, both procedures producing a functional joint or portion thereof.