Abstract: High throughput compound profiling systems, and related devices and sub-systems that can be used to perform various compound profiling processes are provided. These systems typically include work perimeters that are organized for optimum efficiency and processing accuracy. Further, these systems are readily adaptable for performing a wide array of assays, as many different system components are easily incorporated or interchangeable in a particular system. System components that are provided by the invention include cell culture dissociators, which can be used, e.g., to effect cell wetting, dissociation, and/or agitation applications. In some embodiments, these cell culture dissociators are included as components of automated cell culture passaging stations. Dispensing devices that permit on-the-fly fluid temperature regulation are also provided. In addition, various compound profiling methods, cell dissociation methods, uniform cell concentration dispensing methods, among other processes, are also provided.

Abstract: A gel extraction device comprising a hollow cutting member and a plunger ejector member. The hollow cutting member has a proximal tubular body terminating at one end in a circular rim having oppositely disposed spring engaging structures and a distal sloped transition portion ending in a rectangular receptacle and a tapered end portion with a sharp perimeter cutting edge. The plunger ejector member has a proximal cap atop and connected to a split stem portion having outer legs with shoulder stop extensions; an inner ribbon spring leg having a distal end inserted into a spring engaging structure in the assembled device; and a solid rectangular distal portion. In a second embodiment the split end portion is replaced by a stem having a plurality of lateral protrusions and a coiled spring is placed within the hollow cutting member tubular body. A third embodiment is a hybrid of the first two embodiments.