Abstract: A detachable autoclaveable dental unit water line and pre-filter having a water line made of a thermoplastic fluoropolymer tubing that connects to standard dental handset or tools. The fluoropolymer tubing is suitable for sterilization in the typical autoclave units that are used in dental facilities due to the tubing's ability to withstand the high temperatures used in the autoclave. Use of the autoclave facilitates sterilization of active biofilm, which biofilm provides a mechanism for the growth and distribution of bacteria, that has a tendency to accumulate in the water line tubing. The pre-filter is connected at the opposite end of the fluoropolymer tubing from the dental handset or tool and is releasably connected to the water supply to filter out bacteria and other materials in the water supply to prevent recolonization of the biofilm.