Abstract: The Tissue Transfer method consists of transferring intact, organized cells from the surfaces of biological tissues or organs to a transfer substrate. A surface of the tissue or organ is selected, in most cases, a freshly cut surface. At least one layer of intact cells is transferred by adhesion of the cells to a transfer substrate, which is a membrane, film, plate or liquid layer bound to a solid structure. The substrate is brought into contact with the selected surface and removed. A layer of cells is removed by the adhesion of the cells to the substrate and the cells retain the organization of the organ or tissue.

Abstract: The Tissue Transfer method consists of transferring intact, organized cells from the surfaces of biological tissues or organs to a transfer substrate. A surface of the tissue or organ is selected, in most cases, a freshly cut surface. At least one layer of intact cells is transferred by adhesion of the cells to a transfer substrate, which is a membrane, film, plate or liquid layer bound to a solid structure. The substrate is brought into contact with the selected surface and removed. A layer of cells is removed by the adhesion of the cells to the substrate and the cells retain the organization of the organ or tissue. Method and apparatus for retrieving glomeruli dislodged from renal biopsy cores during the biopsy procedure and immobilizing the glomeruli on a membrane substrate. The morphological quality of these glomeruli is such that they can be used for diagnostic evaluation.

Abstract: An isolated nucleic acid is provided which encodes a transiently-expressed kinetochore protein, CENP-F. Also provided are the purified polypeptide encoded by the nucleic acid sequence, and antibodies immunologically specific for the polypeptide. These biological molecules are useful as markers of cellular proliferation, particularly for the identification of cells in the G2 and M phases of the cell cycle. Methods are provided for using the nucleic acid, protein and antibodies for assessing cellular proliferation in biological fluids and tissue samples, and for detecting the presence of autoantibodies to the protein.