Abstract: An apparatus and method for protecting at least one component of a light source are provided. The apparatus includes a shield for the component, with this shield having a plurality of locations that are substantially transparent to an emission wave length of the light source. The shield is positioned such that during use of the light source, one of the substantially transparent locations of the shield is disposed between the at least one component of the light source and an object that is to be irradiated. The apparatus also includes a mechanism for advancing the shield upon activation of the light source or an element thereof in order to be able to dispose a different one of the substantially transparent locations of the shield between the at least one component of the light source and an object that is to be irradiated.

Abstract: A method and apparatus for activating a thermo-enzyme reaction, such as a polymerase chain reaction or other temperature-sensitive transformation of biological systems are provided. Electromagnetic energy is applied to a target to produce a rapid elevation in the temperature of at least a portion of the target. The electromagnetic energy can be laser energy provided via a laser beam supplied from one or more laser sources. The laser beam can have a wavelength in the infrared range from 750 nm to mm. The source of electromagnetic energy can be used in association with a microscope and/or objective lens to irradiate microscopic targets.