Abstract: A product concentration device that utilizes a reservoir connected to a hollow-fiber filter element where the reservoir can serve as a container for filtrate emanating from another filtering device, such that product in the reservoir can be stored, concentrated and/or further processed as desired. Enclosed reactor systems, each of at least three chambers, fluid flow between the chambers controlled by selectively permeable barriers, flow controlled by an alternating flow diaphragm pump.

Abstract: Improved screen filter modules, related compartmentalized filtration modules, and related filtration processes, suitable for filtering fluid to eliminate suspended particulate matter, such as living cells or microcarriers anchoring living cells, or to separate particulate matter based on size. The improvement is the presence of a barrier that channels redirected filtrate to the portion of the filter most susceptible to clogging by the particulate matter and induces flow patterns that act against clogging.

Abstract: A filtration systems for fluids, particularly biological fluids. The filtration system includes a filter containing compartment connected at one end to a storage vessel and at the other end to a diaphragm pump. The filter comprises a hollow fiber module or a screen filter. The vessel serves as a storage container for a process stream to be filtered. The diaphragm pump provides the means for generating rapid, alternating, low shear tangential flow between the vessel and pump and through the hollow fibers or screen filter. The system allows easy removal of wastes from the fluid and the addition of fresh fluid to replenish the filtered fluid.

Abstract: The present invention relates to immunoconjugates comprising an antibody fragment which is covalently bound to a diagnostic or therapeutic principle through a carbohydrate moiety in the light chain variable region of the antibody fragment. The invention also relates to immunoconjugates comprising an antibody moiety that is an intact antibody containing a glycosylation site in the light chain variable domain which has been introduced into the antibody by mutating the nucleotide sequence encoding the light chain. The resultant immunoconjugates retain the immunoreactivity of the antibody fragment or intact antibody, and target the diagnostic or therapeutic principle to a target tissue where the diagnostic or therapeutic effect is realized. Thus, the invention contemplates the use of such immunoconjugates for diagnosis and immunotherapy. The invention further relates to methods for preparing such immunoconjugates.

Abstract: The present invention relates to immunoconjugates comprising an antibody fragment which is covalently bound to a diagnostic or therapeutic principle through a carbohydrate moiety in the light chain variable region of the antibody fragment. The invention also relates to immunoconjugates comprising an antibody moiety that is an intact antibody containing a glycosylation site in the light chain variable domain which has been introduced into the antibody by mutating the nucleotide sequence encoding the light chain. The resultant immunoconjugates retain the immunoreactivity of the antibody fragment or intact antibody, and target the diagnostic or therapeutic principle to a target tissue where the diagnostic or therapeutic effect is realized. Thus, the invention contemplates the use of such immunoconjugates for diagnosis and immunotherapy. The invention further relates to methods for preparing such immunoconjugates.

Abstract: A system for electrophoretic analysis includes four tanks, each of which carries an electrolytic solution which accesses a different edge surface of a pair of parallel gel plates. The gel plates include an electrophoretic separation path formed of a selected medium, e.g. an isoelectric focusing gel, extending in a space between the plates and across opposite edges of the plates. A pair of nonpolar barrier webs extend between the plates parallel to and on each side of the path. A different medium, e.g. a running gel, occupies the areas between the gel plates from the opposite sides of the barriers to the remaining edges of the plates. A pair of gaskets are positioned along plate edges which are perpendicular to the path. The gaskets include access apertures for the path and the barriers. A specimen is positioned within a channel leading from an electrolytic solution tank to the path aperture of a gasket and between a dam and the aperture.