Patents by Inventor Jesper Lohse
Jesper Lohse has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240029409Abstract: There is provided a method for training a ground truth generator machine learning model, comprising: creating a ground truth multi-record training dataset wherein a record comprises: a first image of a sample of tissue of a subject depicting a first group of biological objects, a second image of the sample depicting a second group of biological objects presenting at least one biomarker, and ground truth labels indicating a respective biological object category of a plurality of biological object categories for biological object members of the first group and the second group; and training the ground truth generator machine learning model on the ground truth multi-record training dataset for automatically generating ground truth labels selected from the plurality of biological object categories for biological objects depicted in an input set of images of a first type corresponding to the first image and a second type corresponding to the second image.Type: ApplicationFiled: August 18, 2021Publication date: January 25, 2024Applicant: NEWTRON GROUP SAInventors: Frederik AIDT, Jesper LOHSE, Elad ARBEL, Itay REMER, Amir BEN-DOR, Oded BEN-DAVID
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Publication number: 20230002816Abstract: Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as a rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.Type: ApplicationFiled: August 10, 2022Publication date: January 5, 2023Inventors: Jesper Lohse, Mike Paw Maischnack Hansen
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Patent number: 11466314Abstract: Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as a rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.Type: GrantFiled: November 19, 2019Date of Patent: October 11, 2022Assignee: Agilent Technologies, Inc.Inventors: Jesper Lohse, Mike Paw Maischnack Hansen
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Publication number: 20200292535Abstract: The present invention lies in the field of visualization and quantification of immobilized targets in samples using immunochemical means. In particular, the invention relates to a method and reagents for detection, visualization and quantification of a molecular target in immunosiained histological samples using particular compositions of the target specific binding agent. Methods and compositions of the invention are suitable for any assay that uses a target visualization system in histological samples based on detection of the target by the target specific binding agent. The methods and compositions tire useful for evaluation of targets that are biomarkers of diseases in medical diagnostic.Type: ApplicationFiled: April 2, 2020Publication date: September 17, 2020Inventors: Jesper Lohse, Kristian Jensen, Rikke Malene Krusenst Jerna-Hafstrøm, Kirsten Damgaard Hoff, Lars Christian Jacobsen
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Patent number: 10718777Abstract: The present invention relates to methods of visualizing targets in histological samples, e.g. biopsy samples, wherein the methods comprise staining of the sample with (i) one or more target specific immunochemical stains, and (ii) a histological stain for specific tissue components e.g. iron, mucins glycogen, amyloid, nucleic acids, etc., e.g. hematoxilyn and/or eosin stains or the like, that is used to enhance contrast in the microscopic image of a tissue sample, highlight morphologic structures in the sample for viewing, define and examine tissues, cell populations, or organelles within individual cells. Methods may further comprise evaluation of expression of one or more targets in the sample. The disclosed methods are useful for medical diagnostics.Type: GrantFiled: December 6, 2011Date of Patent: July 21, 2020Assignee: AGILENT TECHNOLOGIES, INC.Inventor: Jesper Lohse
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Publication number: 20200157613Abstract: Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as a rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.Type: ApplicationFiled: November 19, 2019Publication date: May 21, 2020Inventors: Jesper Lohse, Mike Paw Maischnack Hansen
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Patent number: 10648970Abstract: The present invention lies in the field of visualization and quantification of immobilized targets in samples using immunochemical means. In particular, the invention relates to a method and reagents for detection, visualization and quantification of a molecular target in immunostained histological samples using particular compositions of the target specific binding agent. Methods and compositions of the invention are suitable for any assay that uses a target visualization system in histological samples based on detection of the target by the target specific binding agent. The methods and compositions are useful for evaluation of targets that are biomarkers of diseases in medical diagnostic.Type: GrantFiled: November 8, 2012Date of Patent: May 12, 2020Assignee: AGILENT TECHNOLOGIES, INC.Inventors: Jesper Lohse, Kristian Jensen, Rikke Malene Krusenst Jerna-Hafstrom, Kirsten Damgaard Hoff, Lars Christian Jacobsen
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Patent number: 10526646Abstract: Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.Type: GrantFiled: December 19, 2016Date of Patent: January 7, 2020Assignee: Agilent Technologies, Inc.Inventors: Jesper Lohse, Mike Paw Maischnack Hansen
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Publication number: 20190085048Abstract: The present invention describes novel methods to generate MHC multimers and methods to improve existing and new MHC multimers. The invention also describes improved methods for the use of MHC multimers in analysis of T-cells in samples including diagnostic and prognostic methods. Furthermore, the use of MHC multimers in therapy are described, e.g. anti-tumour and anti-virus therapy, including isolation of antigen specific T-cells capable of inactivation or elimination of undesirable targeT-cells or isolation of specific T-cells capable of regulation of other immune cells.Type: ApplicationFiled: June 22, 2018Publication date: March 21, 2019Inventors: Liselotte Brix, Henrik Pedersen, Tina Jakobsen, Jørgen Schøller, Jesper Lohse, Katja Brunstedt, Kivin Jacobsen
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Patent number: 10180426Abstract: The present invention lies in the field of visualization and quantification of immobilized targets in samples using immunochemical means. The methods of the invention utilize an immunostaining system allowing visualizing single target units in samples as distinct dots. In particular, the invention relates to methods and reagents for visualization and quantification of molecular targets immunostained in histological samples and use of said method and reagents in medical diagnostic. However, the visualization and quantification methods of the invention are applicable to a variety of targets in different samples and allow precise quantifying both relative and absolute amounts thereof.Type: GrantFiled: November 8, 2011Date of Patent: January 15, 2019Assignee: DAKO DENMARK A/SInventors: Jesper Lohse, Galina Skladtchikova
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Patent number: 10168335Abstract: The present invention relates to a method of biological labeling that occurs via a free radical chain reaction. The labeling occurs due to deposition of a detectable reporter molecule from a media comprising a substance comprising at least two moieties of a peroxidase enzyme substrate (termed herein ‘cross-linker’) in a target site comprising peroxidase activity and a biological marker. The labeling reaction described herein may generally be used to detect targets in a host of experimental schemes for detecting and visualizing a biological or chemical target, including immunohistochemistry (IHC), in situ hybridization (ISH), antibody-based staining methods such as ELISA, Southern, Northern, and Western blotting, and others.Type: GrantFiled: May 26, 2016Date of Patent: January 1, 2019Assignee: DAKO DENMARK A/SInventors: Jesper Lohse, Kenneth Heesche Petersen
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Patent number: 10036066Abstract: The invention provides compositions and methods for the detection of targets in a sample; in particular, an in situ hybridization (ISH) sample. Probes and detectable labels may be provided in multiple layers in order to increase the flexibility of a detection system, and to allow for amplification to enhance the signal from a target. The layers may be created by incorporating probes and detectable labels into larger molecular units that interact through nucleic acids base-pairing, including peptide-nucleic acid (PNA) base-pairing. Optional non-natural bases allow for degenerate base pairing schemes. The compositions and methods are also compatible with immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry, enzyme immuno-assays (EIA), enzyme linked immuno-assays (ELISA), blotting methods (e.g. Western, Southern, and Northern), labeling inside electrophoresis systems or on surfaces or arrays, and precipitation, among other general detection assay formats.Type: GrantFiled: March 17, 2015Date of Patent: July 31, 2018Assignee: DAKO DENMARK A/SInventors: Lars Winther, Jesper Lohse, Susanne Gabs, Kenneth Heesche Petersen
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Patent number: 10030065Abstract: The present invention relates to a soluble negative control MHC multimer comprising a nonsense peptide that binds the MHC protein efficiently, but that does not support binding of the resultant MHC-peptide complex to the desired T Cell Receptor. The nonsense peptide is designed to i) have a length enabling binding to the MHC allele in question, ii) have appropriate amino acids at relevant anchor positions which anchor the nonsense peptide to the peptide-binding groove of the MHC, iii) have amino acids outside the anchor positions that do not support binding to a T Cell Receptor, and iv) have an amino acid sequence that is different from the linear sequence of any naturally occurring peptide.Type: GrantFiled: December 22, 2009Date of Patent: July 24, 2018Assignee: Dako Denmark A/SInventors: Liselotte Brix, Henrik Pedersen, Tina Jakobsen, Jørgen Schøller, Jesper Lohse, Katja Brunstedt, Kivin Jacobsen
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Methods and systems for analyzing images of specimens processed by a programmable quantitative assay
Patent number: 9970874Abstract: Disclosed are methods and systems for analyzing images of specimens processed by a programmable quantitative assay or more specifically a robust programmable quantitative dot assay, PDQA, that enable specimens to be imaged and assessed across a wide variety of conditions and applications. Specific embodiments directed to immunohistochemical applications provide more quantitative methods of imaging and assessing biological samples including tissue samples.Type: GrantFiled: November 29, 2011Date of Patent: May 15, 2018Assignee: DAKO DENMARK A/SInventors: Jesper Lohse, Hans Christian Pedersen, Joachim Schmid, Jeff Caron, Rohit Jain, Thomas Briscoe -
Patent number: 9958449Abstract: The present invention relates to methods and compounds for enzyme-mediated amplification of target-associated signals for visualization of biological or chemical targets in samples, wherein the targets are immobilized, in particular the invention relates to the oxidoreductase-mediated signal amplification for visualization of targets in samples comprising cells. The methods of the invention are particular useful for qualitative and quantitative evaluation of biological markers relating to medical diagnostic and therapy.Type: GrantFiled: May 26, 2016Date of Patent: May 1, 2018Assignee: DAKO DENMARK A/SInventors: Jesper Lohse, Galina Skladchikova
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Publication number: 20170175178Abstract: Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.Type: ApplicationFiled: December 19, 2016Publication date: June 22, 2017Inventors: Jesper Lohse, Mike Paw Maischnack Hansen
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Patent number: 9671400Abstract: The present invention relates to compounds which are conjugates of two or more moieties capable of serving as substrates of an enzyme with peroxidase activity and one or more labels. The conjugate molecules of the invention are useful for detection of molecular targets, e.g. biological or chemical molecules, molecular structures, etc, in samples using a host of experimental schemes for detecting and visualizing such targets, e.g. immunohistochemistry (IHC), in situ hybridization (ISH), ELISA, Southern, Northern, and Western blotting, etc. The invention also relates to compositions comprising the conjugate molecules.Type: GrantFiled: February 19, 2010Date of Patent: June 6, 2017Assignee: Dako Denmark A/SInventor: Jesper Lohse
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Patent number: 9598720Abstract: The present invention concerns monomeric or polymeric linker molecules useful in biological and chemical applications, their synthesis, and the synthesis and use of derivatives of the linkers conjugated to a variety of detectable labels and other substances. The linkers may be used, for example, in conjunction with fluorescent labels, nucleic acid or nucleic acid analog probes, and solid phase systems, and to enhance the solubility of the conjugated molecules.Type: GrantFiled: August 21, 2013Date of Patent: March 21, 2017Assignee: DAKO DENMARK A/SInventor: Jesper Lohse
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Publication number: 20160370375Abstract: The present invention relates to a method of biological labeling that occurs via a free radical chain reaction. The labeling occurs due to deposition of a detectable reporter molecule from a media comprising a substance comprising at least two moieties of a peroxidase enzyme substrate (termed herein ‘cross-linker’) in a target site comprising peroxidase activity and a biological marker. The labeling reaction described herein may generally be used to detect targets in a host of experimental schemes for detecting and visualizing a biological or chemical target, including immunohistochemistry (IHC), in situ hybridization (ISH), antibody-based staining methods such as ELISA, Southern, Northern, and Western blotting, and others.Type: ApplicationFiled: May 26, 2016Publication date: December 22, 2016Inventors: Jesper Lohse, Kenneth Heesche Petersen
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Publication number: 20160273035Abstract: The invention provides compositions and methods for the detection of targets in a sample; in particular, an in situ hybridization (ISH) sample. Probes and detectable labels may be provided in multiple layers in order to increase the flexibility of a detection system, and to allow for amplification to enhance the signal from a target. The layers may be created by incorporating probes and detectable labels into larger molecular units that interact through nucleic acids base-pairing, including peptide-nucleic acid (PNA) base-pairing. Optional non-natural bases allow for degenerate base pairing schemes. The compositions and methods are also compatible with immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry, enzyme immuno-assays (EIA), enzyme linked immuno-assays (ELISA), blotting methods (e.g. Western, Southern, and Northern), labeling inside electrophoresis systems or on surfaces or arrays, and precipitation, among other general detection assay formats.Type: ApplicationFiled: March 17, 2015Publication date: September 22, 2016Applicant: DAKO DENMARK A/SInventors: Lars Winther, Jesper Lohse, Susanne Gabs, Kenneth Heesche Petersen