Patents by Inventor Jesper Ulf Hansson

Jesper Ulf Hansson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240018184
    Abstract: The present invention concerns a method of cleaning and/or sanitizing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) optionally purifying a mixture comprising a first immunoglobulin using the separation matrix; b) providing a cleaning liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; and c) cleaning and/or sanitizing the separation matrix by contacting the cleaning liquid with the separation matrix for a predetermined contact time.
    Type: Application
    Filed: July 18, 2023
    Publication date: January 18, 2024
    Inventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Patent number: 11753438
    Abstract: The present invention concerns a method of cleaning and/or sanitizing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) optionally purifying a mixture comprising a first immunoglobulin using the separation matrix; b) providing a cleaning liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; and c) cleaning and/or sanitizing the separation matrix by contacting the cleaning liquid with the separation matrix for a predetermined contact time.
    Type: Grant
    Filed: May 10, 2017
    Date of Patent: September 12, 2023
    Assignee: CYTIVA BIOPROCESS R&D AB
    Inventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Publication number: 20230279047
    Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
    Type: Application
    Filed: April 11, 2023
    Publication date: September 7, 2023
    Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Publication number: 20230257417
    Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support: b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing said separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA).
    Type: Application
    Filed: March 10, 2023
    Publication date: August 17, 2023
    Inventors: Annika Kristina Forss, Gustav Jose Rodrigo, Tomas Bjorkman, Jesper Ulf Hansson, Mats Ander
  • Patent number: 11708390
    Abstract: The present invention concerns a method of storing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) providing a storage liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; b) permeating the separation matrix with the storage liquid; and c) storing the storage liquid-permeated separation matrix for a storage time of at least days.
    Type: Grant
    Filed: May 10, 2017
    Date of Patent: July 25, 2023
    Assignee: Cytiva BioProcess R&D AB
    Inventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Patent number: 11685764
    Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50, v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
    Type: Grant
    Filed: April 2, 2021
    Date of Patent: June 27, 2023
    Assignee: CYTIVA BIOPROCESS R&D AB
    Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Patent number: 11623941
    Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 51 or SEQ ID NO: 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO: 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO: 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leuc
    Type: Grant
    Filed: May 27, 2020
    Date of Patent: April 11, 2023
    Assignee: CYTIVA BIOPROCESS R&D AB
    Inventors: Annika Kristina Forss, Gustav Jose Rodrigo, Tomas Bjorkman, Jesper Ulf Hansson, Mats Ander
  • Publication number: 20210221841
    Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
    Type: Application
    Filed: April 2, 2021
    Publication date: July 22, 2021
    Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Publication number: 20210187476
    Abstract: The present invention relates to chromatography beads, production and use thereof. More closely the invention relates to small, rigid and nan-permeable agarose beads suitable for example as stationary phase in high performance liquid chromatography (HPLC) for analyses of biomolecules, such as, peptides and proteins; and methods for producing such beads.
    Type: Application
    Filed: June 25, 2019
    Publication date: June 24, 2021
    Inventors: Johan Fredrik Öhman, Jesper Ulf Hansson, Jasmin Faroque, Eva Holmgren, David Bror Lennart Jansson
  • Patent number: 10995113
    Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
    Type: Grant
    Filed: June 5, 2020
    Date of Patent: May 4, 2021
    Assignee: Cytiva BioProcess R&D AB
    Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Publication number: 20200299326
    Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
    Type: Application
    Filed: June 5, 2020
    Publication date: September 24, 2020
    Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Publication number: 20200299325
    Abstract: The present invention concerns a method of cleaning and/or sanitizing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) optionally purifying a mixture comprising a first immunoglobulin using the separation matrix; b) providing a cleaning liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; and c) cleaning and/or sanitizing the separation matrix by contacting the cleaning liquid with the separation matrix for a predetermined contact time.
    Type: Application
    Filed: May 10, 2017
    Publication date: September 24, 2020
    Inventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Publication number: 20200283475
    Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 51 or SEQ ID NO: 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO: 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO: 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leuc
    Type: Application
    Filed: May 27, 2020
    Publication date: September 10, 2020
    Inventors: Annika Kristina Forss, Gustav Jose Rodrigo, Tomas Bjorkman, Jesper Ulf Hansson, Mats Ander
  • Publication number: 20200239517
    Abstract: The present invention concerns a method of storing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) providing a storage liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; b) permeating the separation matrix with the storage liquid; and c) storing the storage liquid-permeated separation matrix for a storage time of at least days.
    Type: Application
    Filed: May 10, 2017
    Publication date: July 30, 2020
    Inventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Patent number: 10711035
    Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
    Type: Grant
    Filed: November 13, 2019
    Date of Patent: July 14, 2020
    Assignee: GE HEALTHCARE BIOPROCESS R&D AB
    Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Patent number: 10703774
    Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by, or having at least 80% such as at least 90%, 95% or 98% identity to, SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting of
    Type: Grant
    Filed: November 10, 2016
    Date of Patent: July 7, 2020
    Assignee: GE Healthcare BioProcess R&D AB
    Inventors: Annika Kristina Forss, Gustav Jose Rodrigo, Tomas Bjorkman, Jesper Ulf Hansson, Mats Ander
  • Patent number: 10654887
    Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
    Type: Grant
    Filed: September 30, 2016
    Date of Patent: May 19, 2020
    Assignee: GE Healthcare Bio-Process R&D AB
    Inventors: Gustav Jose Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Publication number: 20200071353
    Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
    Type: Application
    Filed: November 13, 2019
    Publication date: March 5, 2020
    Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Patent number: 10513537
    Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
    Type: Grant
    Filed: May 10, 2017
    Date of Patent: December 24, 2019
    Assignee: GE Healthcare BioProcess R&D AB
    Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
  • Publication number: 20190119318
    Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
    Type: Application
    Filed: May 10, 2017
    Publication date: April 25, 2019
    Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson