Patents by Inventor Jesper Ulf Hansson
Jesper Ulf Hansson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20240018184Abstract: The present invention concerns a method of cleaning and/or sanitizing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) optionally purifying a mixture comprising a first immunoglobulin using the separation matrix; b) providing a cleaning liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; and c) cleaning and/or sanitizing the separation matrix by contacting the cleaning liquid with the separation matrix for a predetermined contact time.Type: ApplicationFiled: July 18, 2023Publication date: January 18, 2024Inventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Patent number: 11753438Abstract: The present invention concerns a method of cleaning and/or sanitizing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) optionally purifying a mixture comprising a first immunoglobulin using the separation matrix; b) providing a cleaning liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; and c) cleaning and/or sanitizing the separation matrix by contacting the cleaning liquid with the separation matrix for a predetermined contact time.Type: GrantFiled: May 10, 2017Date of Patent: September 12, 2023Assignee: CYTIVA BIOPROCESS R&D ABInventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Publication number: 20230279047Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: ApplicationFiled: April 11, 2023Publication date: September 7, 2023Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Publication number: 20230257417Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support: b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing said separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA).Type: ApplicationFiled: March 10, 2023Publication date: August 17, 2023Inventors: Annika Kristina Forss, Gustav Jose Rodrigo, Tomas Bjorkman, Jesper Ulf Hansson, Mats Ander
-
Patent number: 11708390Abstract: The present invention concerns a method of storing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) providing a storage liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; b) permeating the separation matrix with the storage liquid; and c) storing the storage liquid-permeated separation matrix for a storage time of at least days.Type: GrantFiled: May 10, 2017Date of Patent: July 25, 2023Assignee: Cytiva BioProcess R&D ABInventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Patent number: 11685764Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50, v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: GrantFiled: April 2, 2021Date of Patent: June 27, 2023Assignee: CYTIVA BIOPROCESS R&D ABInventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Patent number: 11623941Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 51 or SEQ ID NO: 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO: 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO: 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucType: GrantFiled: May 27, 2020Date of Patent: April 11, 2023Assignee: CYTIVA BIOPROCESS R&D ABInventors: Annika Kristina Forss, Gustav Jose Rodrigo, Tomas Bjorkman, Jesper Ulf Hansson, Mats Ander
-
Publication number: 20210221841Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: ApplicationFiled: April 2, 2021Publication date: July 22, 2021Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Publication number: 20210187476Abstract: The present invention relates to chromatography beads, production and use thereof. More closely the invention relates to small, rigid and nan-permeable agarose beads suitable for example as stationary phase in high performance liquid chromatography (HPLC) for analyses of biomolecules, such as, peptides and proteins; and methods for producing such beads.Type: ApplicationFiled: June 25, 2019Publication date: June 24, 2021Inventors: Johan Fredrik Öhman, Jesper Ulf Hansson, Jasmin Faroque, Eva Holmgren, David Bror Lennart Jansson
-
Patent number: 10995113Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: GrantFiled: June 5, 2020Date of Patent: May 4, 2021Assignee: Cytiva BioProcess R&D ABInventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Publication number: 20200299326Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: ApplicationFiled: June 5, 2020Publication date: September 24, 2020Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Publication number: 20200299325Abstract: The present invention concerns a method of cleaning and/or sanitizing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) optionally purifying a mixture comprising a first immunoglobulin using the separation matrix; b) providing a cleaning liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; and c) cleaning and/or sanitizing the separation matrix by contacting the cleaning liquid with the separation matrix for a predetermined contact time.Type: ApplicationFiled: May 10, 2017Publication date: September 24, 2020Inventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Publication number: 20200283475Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 51 or SEQ ID NO: 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO: 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO: 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucType: ApplicationFiled: May 27, 2020Publication date: September 10, 2020Inventors: Annika Kristina Forss, Gustav Jose Rodrigo, Tomas Bjorkman, Jesper Ulf Hansson, Mats Ander
-
Publication number: 20200239517Abstract: The present invention concerns a method of storing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) providing a storage liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; b) permeating the separation matrix with the storage liquid; and c) storing the storage liquid-permeated separation matrix for a storage time of at least days.Type: ApplicationFiled: May 10, 2017Publication date: July 30, 2020Inventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Patent number: 10711035Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: GrantFiled: November 13, 2019Date of Patent: July 14, 2020Assignee: GE HEALTHCARE BIOPROCESS R&D ABInventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Patent number: 10703774Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by, or having at least 80% such as at least 90%, 95% or 98% identity to, SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting ofType: GrantFiled: November 10, 2016Date of Patent: July 7, 2020Assignee: GE Healthcare BioProcess R&D ABInventors: Annika Kristina Forss, Gustav Jose Rodrigo, Tomas Bjorkman, Jesper Ulf Hansson, Mats Ander
-
Patent number: 10654887Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: GrantFiled: September 30, 2016Date of Patent: May 19, 2020Assignee: GE Healthcare Bio-Process R&D ABInventors: Gustav Jose Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Publication number: 20200071353Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: ApplicationFiled: November 13, 2019Publication date: March 5, 2020Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Patent number: 10513537Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: GrantFiled: May 10, 2017Date of Patent: December 24, 2019Assignee: GE Healthcare BioProcess R&D ABInventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
-
Publication number: 20190119318Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: ApplicationFiled: May 10, 2017Publication date: April 25, 2019Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson