Abstract: The present invention is directed to phospholipase from Fusarium solani (MUCL38667) and analogues thereof, nucleic acid constructs, recombinant expression vectors, and recombinant host comprising the nucleic acid sequences encoding the phospholipase from Fusarium solani (MUCL38667) and analogues thereof, methods of making same, and method of using the phosopholipases of the present invention in baking methods, baking compositions and detergent compositions.

Abstract: The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.

Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.

Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability.

Abstract: A method for the construction of a library of recombined polynucleotides from a number of different starting single or double stranded parental DNA templates is disclosed, wherein the starting single or double stranded parental DNA templates represent discrete points in a population of genes encoding evolutionary or synthetic homologues of a peptide having homologies ranging over a broad spectrum from less than 15% to more than 80%, said population exhibiting at least one identification sequence, and whereby said genes are subjected to a gene shuffling procedure to generate shuffled mutants of said population of genes representing additional discrete points between those of said starting templates.

Abstract: A method for in vitro construction of a library of recombined homologous polynucleotides from a number of different starting DNA templates and primers by induced template shifts during an polynucleotide synthesis is described, wherebyA. extended primers are synthesized bya) denaturing the DNA templatesb) annealing primers to the templates,c) extending the said primers by use of a polymerase,d) stop the synthesis, ande) separate the extended primers from the templates,B. a template shift is induced bya) isolating the extended primers from the templates and repeating steps A.b) to A.e) using the extended primers as both primers and templates, orb) repeating steps A.b) to A.e),C. this process is terminated after an appropriate number of cycles of process steps A. and B.a), A. and B.b), or combinations thereof.Optionally the polynucleotides are amplified in a standard PCR reaction with specific primers to selectively amplify homologous polynucleotides of interest.

Abstract: Cellulose-containing fabric is desized by treating with an enzyme hybrid having a catalytically active amino acid sequence of an enzyme such as a lipase or an amylase linked to an amino acid sequence containing a cellulose-binding domain. The enzyme amino acid sequence may be of an .alpha.-amylase obtainable from a species of Bacillus such as Bacillus licheniformis, or of a lipase obtainable from a species of Humicola, Candida, Pseudomonas or Bacillus. The cellulose-binding domain may be from a cellulase, a xylanase, a mannanase, an arabinofuranosidase, an acetylesterase or a chitinase. The enzyme hybrid is obtained from a transformed host cell containing an expression cassette having a DNA sequence encoding the enzyme hybrid. A desizing composition is formed containing the enzyme hybrid and a wetting agent.

Abstract: The present invention relates to a process for removal or bleaching of soiling or stains present on cellulosic fabric, wherein the fabric is contacted in aqueous medium with a modified enzyme (enzyme hybrid) which comprises a catalytically active amino acid sequence of a non-cellulolytic enzyme linked to an amino acid sequence comprising a cellulose-binding domain. The invention further relates to a detergent composition comprising an enzyme hybrid of the type in question and a surfactant, and to a process for washing soiled or stained cellulosic fabric, wherein the fabric is washed in an aqueous medium to which is added such a detergent composition.

Abstract: The present invention relates to a novel variant of peroxidase with improved stability at alkaline conditions, and a bleaching or detergent composition comprising the peroxidase variant.