Patents by Inventor Jess Sustarich

Jess Sustarich has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230036493
    Abstract: The present disclosure relates to a high throughput, scalable system for electroporation of biological cells. The two part system comprises an electroporation reaction array that interfaces with a control unit, providing electrical pulse, temperature control, and mixing of the sample contents. The control unit automates the entire process; electroporation, cell recovery and outgrowth are performed in a electroporation array assembly. The bottom of each reaction well of the electroporation array assembly contains a pair of coplanar electrodes. The bottom surface containing the electrodes is treated in a way to render it hydrophilic. This results in increased wetting of the electrodes thereby increasing transformation efficiency. The electrode configuration allows for processing of smaller sample volumes, reducing the consumption of expensive biological reagents by several orders of magnitude compared to conventional cuvette-based electroporation devices.
    Type: Application
    Filed: July 29, 2022
    Publication date: February 2, 2023
    Inventors: Jess Sustarich, Anup K. Singh, Lauren Washburn, William Gaillard
  • Publication number: 20210205813
    Abstract: Disclosed herein include systems, devices, and methods for generating and directly loading droplets onto a microfluidic device with contactless delivery. Droplets can be generated and loaded onto a sealed microfluidic device through one or more connecting ports. Loaded droplets can be manipulated, such as merged.
    Type: Application
    Filed: December 22, 2020
    Publication date: July 8, 2021
    Inventors: Kosuke Iwai, Jess Sustarich, William Randy Gaillard, Anup Kumar Singh
  • Publication number: 20170114398
    Abstract: Embodiments of the present invention relate to systems, methods and devices for the detection of nucleic acids. Some embodiments relate to portable devices comprising nanochannels for efficient detection of a nucleic acid comprising a target polynucleotide sequence in a sample, and use thereof. More embodiments include detection methods in which a sample and one or more nucleic acid probes are introduced into a channel. A first potential difference is applied across the length of the channel in a first direction, and a first electrical property value is detected. Subsequently, a second potential difference is applied across the length of the channel in a second opposite direction, and a second electrical property value is detected. Presence or absence of a nucleic acid in the channel is determined based on a comparison between the first and second electrical property values.
    Type: Application
    Filed: October 26, 2016
    Publication date: April 27, 2017
    Inventors: Ronald Phillip Chiarello, Sumita Pennathur, Jess Sustarich