Abstract: A serial dilation system includes a plurality of serial dilators that can be advanced over each other so as to dilate anatomical soft tissue along a trajectory toward a target anatomical site. An access cannula can then be introduced over the serial dilators. The serial dilators can be coupled to each other, such that once the access cannula is in place, removal of one of the serial dilators from the access cannula causes all of the serial dilators to be removed from the access cannula.