Abstract: This disclosure relates to methods of managing cancer using bis-biguanide compounds, such as alexidine, and pharmaceutical compositions comprising the same. In certain embodiments, this disclosure relates to methods of treating lung cancer comprising administering an effective amount of a bis-biguanide compound to a subject in need thereof. In certain embodiments, this disclosure relates to methods of treating small cell lung cancer comprising administering an effective amount of a bis-biguanide compound to a subject in need thereof. In certain embodiments, the subject is diagnosed with a lung cancer. In certain embodiments, the subject is diagnosed with small cell lung cancer. In certain embodiments, the subject is a human subject.

Abstract: This disclosure relates to method of isolating rare cells, e.g. unique cancer cells. In certain embodiments, the disclosure contemplates methods of identifying unique cells that are in a sample that contains a group of cells that are replicating at unique locations and/or at different rates. In certain embodiments the uniquely isolated and/or identified cells are evaluated for genetic content and/or expression for diagnostic evaluations. In certain embodiments, the disclosure contemplates compositions comprising cells that are derived from methods disclosed herein.