Abstract: Devices, and methods of use thereof, are provided for the collection of a biological sample during insertion into a human orifice. The device may include a body piece, an insertion piece extending from one end of the body piece, and a handle connected to the other end of the body piece. Upon rotation of the handle, a shaft housed within the body piece, with a collection end at a distal portion thereof, is extended from a retracted position to an extended position, such that the collection end exits an opening within the insertion piece. The angle of the body piece relative to the insertion piece may be configured for self-sampling of a vaginal sample in a seated position. The collection end may be a swab with a plurality of pinwheeling fins having leading edge oriented to collect the biological sample when the shaft is rotated in a given direction.