Patents by Inventor Jia Liu Wolfe

Jia Liu Wolfe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Engineered thioredoxin-like fold proteins
    Patent number: 9340584
    Abstract: The invention features compositions based on thioredoxin-like fold protein domains described as engineered thioredoxin-like fold proteins (ETRXs). These proteins include one or more artificially diversified thioredoxin-like fold protein domains; each domain may be originated from the same or different thioredoxin-like fold protein domains. Features of the invention also include methods for identifying and preparing an enriched composition of target binding, loop-diversified ETRXs with additional sequence variations to improve affinity, stability, selectivity, or solubility. The invention also features compositions of ETRXs substituted with prosthetic groups, polymers, proteins, nucleic acids, carbohydrates, metals, natural or synthetic small molecules and toxins.
    Type: Grant
    Filed: March 29, 2012
    Date of Patent: May 17, 2016
    Assignee: The General Hospital Corporation
    Inventors: Jia Liu Wolfe, Glen S. Cho, Brian Seed
  • Methods, compositions, and kits for the selective activation of protoxins through combinatorial targeting
    Patent number: 8993295
    Abstract: The present invention provides methods and compositions for treating various diseases through selective killing of targeted cells using a combinatorial targeting approach. The invention features protoxin fusion proteins containing a cell targeting moiety and, a modifiable activation moiety which is activated by an activation moiety not naturally operably found in, on, or in the vicinity of a target cell. These methods also include the combinatorial use of two or more therapeutic agents, at minimum comprising a protoxin and a protoxin activator, to target and destroy a specific cell population.
    Type: Grant
    Filed: July 20, 2007
    Date of Patent: March 31, 2015
    Assignee: The General Hospital Corporation
    Inventors: Brian Seed, Jia Liu Wolfe, Glen S. Cho, Chia-Iun Tsai
  • ENGINEERED THIOREDOXIN-LIKE FOLD PROTEINS
    Publication number: 20140113832
    Abstract: The invention features compositions based on thioredoxin-like fold protein domains described as engineered thioredoxin-like fold proteins (ETRXs). These proteins include one or more artificially diversified thioredoxin-like fold protein domains; each domain may be originated from the same or different thioredoxin-like fold protein domains. Features of the invention also include methods for identifying and preparing an enriched composition of target binding, loop-diversified ETRXs with additional sequence variations to improve affinity, stability, selectivity, or solubility. The invention also features compositions of ETRXs substituted with prosthetic groups, polymers, proteins, nucleic acids, carbohydrates, metals, natural or synthetic small molecules and toxins.
    Type: Application
    Filed: March 29, 2012
    Publication date: April 24, 2014
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventors: Jia Liu Wolfe, Glen S. Cho, Brian Seed
  • Fusion proteins comprising a fragment of exotoxin A
    Patent number: 8524241
    Abstract: The invention features recombinant exotoxins from Vibrio cholerae are for the therapeutic treatment of a variety of human diseases, particularly diseases characterized by an abundance or excess of undesired cells.
    Type: Grant
    Filed: July 18, 2008
    Date of Patent: September 3, 2013
    Assignee: The General Hospital Corporation
    Inventors: Brian Seed, Jia Liu Wolfe, Chia-Lun Tsai
  • Fusion proteins comprising a fragment of exotoxin A
    Patent number: 8420094
    Abstract: The invention features recombinant exotoxins from Vibrio cholerae are for the therapeutic treatment of a variety of human diseases, particularly diseases characterized by an abundance or excess of undesired cells.
    Type: Grant
    Filed: July 18, 2008
    Date of Patent: April 16, 2013
    Assignee: The General Hospital Corporation
    Inventors: Brian Seed, Jia Liu Wolfe, Chia-Lun Tsai
  • RECOMBINANT VIBRIO CHOLERAE EXOTOXINS
    Publication number: 20100256070
    Abstract: The invention features recombinant exotoxins from Vibrio cholerae are for the therapeutic treatment of a variety of human diseases, particularly diseases characterized by an abundance or excess of undesired cells.
    Type: Application
    Filed: July 18, 2008
    Publication date: October 7, 2010
    Inventors: Brian Seed, Jia Liu Wolfe, Chia-Lun Tsai
  • METHODS, COMPOSITIONS, AND KITS FOR THE SELECTIVE ACTIVATION OF PROTOXINS THROUGH COMBINATORAL TARGETING
    Publication number: 20100055761
    Abstract: The present invention provides methods and compositions for treating various diseases through selective killipg of targeted cells using a combinatorial targeting approach. The invention features protoxin fusion proteins containing a cell targeting moiety and, a modifiable activation moiety which is activated by an activation moiety not naturally operably found in, on, or in the vicinity of a target cell. These methods also include the combinatorial use of two or more therapeutic agents, at minimum comprising a protoxin and a protoxin activator, to target and destroy a specific cell population.
    Type: Application
    Filed: July 20, 2007
    Publication date: March 4, 2010
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventors: Brian Seed, Jia Liu Wolfe, Glen S. Cho, Chia-lun Tsai
  • Base-modified nucleotides and their use for polymorphism detection
    Patent number: 6994998
    Abstract: The present invention relates to methods for the detection of polymorphisms by substituting a base-modified nucleotide for a natural nucleotide in a polynucleotide. The base-modified nucleotide renders the polynucleotide more susceptible to cleavage at the sites of its incorporation than site consisting of natural nucleotides. The fragments obtained are then analyzed to determine the presence or absence of a polymorphism.
    Type: Grant
    Filed: January 8, 2002
    Date of Patent: February 7, 2006
    Assignee: Sequenom, Inc.
    Inventors: Vincent P. Stanton, Jr., Jia Liu Wolfe, Tomohiko Kawate, Charles Allerson, Gregory L. Verdine
  • Fluorescence-based genotyping
    Patent number: 6855500
    Abstract: The present invention relates to a method for genotyping a diploid organism to detect single nucleotide polymorphisms (SNPs) using modified nucleotides or nucleotide residues substituted with fluorescent groups.
    Type: Grant
    Filed: March 26, 2002
    Date of Patent: February 15, 2005
    Assignee: Sequenom, Inc.
    Inventors: Jia Liu Wolfe, Tomohiko Kawate, Charles R. Allerson, Vincent P. Stanton, Jr.
  • Method for identifying polymorphisms
    Patent number: 6825009
    Abstract: The present invention relates to methods for the detection of polymorphism in polynucleotides by using hybridization of fragments of segments of a polynucleotide suspected of containing a polymorphism with an oligonucleotide having a sequence complementary to a fragment identifying the polymorphism and subsequent detection of incorporated labels in the oligonucleotide-fragment duplex.
    Type: Grant
    Filed: March 22, 2002
    Date of Patent: November 30, 2004
    Assignee: Variagenics, Inc.
    Inventors: Vince P. Stanton, Jr., Jia Liu Wolfe, Tomohiko Kawate, Gregory L. Verdine, Jeffrey Olson, Colin W Dykes
  • Genotyping by mass spectrometric analysis of allelic fragments
    Patent number: 6777188
    Abstract: The present invention relates to a method for genotyping a diploid organism by cleaving segments of two alleles such that 7-20 nucleotide fragments that contain a suspected polymorphic locus are produced and comparing the masses of those fragments.
    Type: Grant
    Filed: March 26, 2002
    Date of Patent: August 17, 2004
    Assignee: Variagenics, Inc.
    Inventors: Vincent P. Stanton, Jr., Jeffrey Olson, Jia Liu Wolfe, Martin Zillmann
  • Fluorescence-based genotyping
    Publication number: 20030165880
    Abstract: The present invention relates to a method for genotyping a diploid organism to detect single nucleotide polymorphisms (SNPs) using modified nucleotides or nucleotide residues substituted with fluorescent groups.
    Type: Application
    Filed: March 26, 2002
    Publication date: September 4, 2003
    Inventors: Jia Liu Wolfe, Tomohiko Kawate, Charles R. Allerson, Vincent P. Stanton
  • Base-modified nucleotides and cleavage of polynucleotides incorporating them
    Patent number: 6610492
    Abstract: The present invention is directed to novel base-modified nucleotides and methods for their use in the preparation and cleavage of modified polynucleotides.
    Type: Grant
    Filed: January 8, 2002
    Date of Patent: August 26, 2003
    Assignee: Variagenics, Inc.
    Inventors: Vincent P. Stanton, Jr., Jia Liu Wolfe, Tomohiko Kawate, Charles Allerson, Gregory L. Verdine
  • Method for identifying polymorphisms
    Publication number: 20030134290
    Abstract: The present invention relates to methods for the detection of polymorphism in polynucleotides by using hybridization of fragments of segments of a polynucleotide suspected of containing a polymorphism with an oligonucleotide having a sequence complementary to a fragment identifying the polymorphism and subsequent detection of incorporated labels in the oligonucleotide-fragment duplex.
    Type: Application
    Filed: March 22, 2002
    Publication date: July 17, 2003
    Inventors: Vince P. Stanton, Jia Liu Wolfe, Tomohiko Kawate, Gregory L. Verdine, Jeffrey Olson
  • Method for analyzing polynucleotides
    Patent number: 6582923
    Abstract: The present invention relates to a method of synthesizing a polynucleotide with incorporation of a modified nucleotide.
    Type: Grant
    Filed: March 22, 2002
    Date of Patent: June 24, 2003
    Assignee: Variagenics, Inc.
    Inventors: Vincent P. Stanton, Jr., Jia Liu Wolfe, Tomohiko Kawate, Gregory L. Verdine
  • Method for analyzing polynucleotides
    Patent number: 6566059
    Abstract: The present invention relates to methods for the analysis of polynucleotides including detection of variance in nucleotide sequence without the need for full sequence determination, full sequence determination of a polynucleotide, genotyping of DNA and labeling a polynucleotide fragment during the process of cleaving it into fragments.
    Type: Grant
    Filed: September 10, 1999
    Date of Patent: May 20, 2003
    Assignee: Variagenics, Inc.
    Inventors: Vincent P. Stanton, Jr., Jia Liu Wolfe, Gregory L. Verdine
  • Method for analyzing polynucleotides
    Publication number: 20030087398
    Abstract: The present invention relates to a method of synthesizing a polynucleotide with incorporation of a modified nucleotide.
    Type: Application
    Filed: March 22, 2002
    Publication date: May 8, 2003
    Inventors: Vincent P. Stanton, Jia Liu Wolfe, Tomohiko Kawate, Gregory L. Verdine
  • Method for identifying polymorphisms
    Patent number: 6500650
    Abstract: The present invention relates to methods for the detection of polymorphism in polynucleotides by using hybridization of fragments of segments of a polynucleotide suspected of containing a polymorphism with an oligonucleotide having a sequence complementary to a fragment identifying the polymorphism and subsequent detection of incorporated labels in the oligonucleotide-fragment duplex.
    Type: Grant
    Filed: September 5, 2000
    Date of Patent: December 31, 2002
    Assignee: Variagenics, Inc.
    Inventors: Vince P. Stanton, Jr., Jia Liu Wolfe, Tomohiko Kawate, Gregory L. Verdine, Jeffrey Olson
  • Genotyping by mass spectrometric analysis of allelic fragments
    Publication number: 20020150943
    Abstract: The present invention relates to a method for genotyping a diploid organism by cleaving segments of two alleles such that 7-20 nucleotide fragments that contain a suspected polymorphic locus are produced and comparing the masses of those fragments.
    Type: Application
    Filed: March 26, 2002
    Publication date: October 17, 2002
    Applicant: Variagenics, Inc.
    Inventors: Vincent P. Stanton, Jeffrey Olson, Jia Liu Wolfe, Martin Zillmann
  • Method for analyzing polynucleotides
    Patent number: 6458945
    Abstract: The present invention relates to methods for the analysis of polynucleotides including detection of variance in nucleotide sequence without the need for full sequence determination, full sequence determination of a polynucleotide, genotyping of DNA and labeling a polynucleotide fragment during the process of cleaving it into fragments.
    Type: Grant
    Filed: November 9, 2000
    Date of Patent: October 1, 2002
    Assignee: Variagenics, Inc.
    Inventors: Vincent P. Stanton, Jr., Jia Liu Wolfe, Tomohiko Kawate, Gregory L. Verdine