Abstract: A positioning method and system are provided. The positioning method includes the steps of utilizing at least one electronic device to establish a calibration database corresponding to an irregular grid of reference location points which are defined in an environment; utilizing a mobile device to detect Wi-Fi access points in the environment; utilizing the mobile device to estimate the signal strengths of the detected Wi-Fi access points to generate an estimated result; utilizing a positioning terminal to compare the estimated result and data stored in the calibration database by a weighed K-Nearest neighbor location algorithm to generate a comparison result; and utilizing the positioning terminal to estimate the location of the mobile device according to the comparison result.